Vol 70, No 4 (2015)

As it became known recently, in addition to the enzyme (enzymes and/or ribozymes) in living organisms occur a large number of ordinary chemical reactions without the participation of biological catalysts. These reactions are distinguished by low speed and, as a rule, the irreversibility. For example, along with diabetes mellitus, glycation and fructosilation of proteins are observed resulted in posttranslational modification with the low- or nonfunctioning protein formation which is poorly exposed to enzymatic proteolysis and therefore accumulates in the body. In addition, the known processes such as the nonenzymatic carbomoylation, pyridoxylation and thiamiation proteins. There is a reasonable basis to believe that alcoholic injury also realized through parametabolic secondary metabolites synthesis such as acetaldehyde. At the same time, the progress in supramolecular chemistry proves that in biological objects there is another large group of parametabolic reactions caused by the formation of supramolecular complexes. Obviously, known parameterizes interactions can modify the formation of supramolecular complexes in living objects. These processes are of considerable interest for fundamental biology and fundamental and practical medicine, but they remain unexplored due to a lack of awareness of a wide range of researchers.

Objective: The aim of this experimental study was to examine the effect of the antioxidant drug U-74389G in a rat model of hypoxia – reoxygenation using the previously established protocol. Effects of treatments were evaluated by magnesium (Mg2+) levels in blood.

Methods: Nonrandomized controlled study was performed. Mg2+ levels were determined in 60 min (groups A and C) and 120 min (groups B and D) after starting the reoxygenation. Groups A and B received no drugs, whereas rats from groups C and D were administered with U-74389G.

Results: 40 rats 16–18 weeks old of a mean weight of 2312 g were employed in the study. It is demonstrated that U-74389G administration did not alter the Mg2+ levels (decrease in Mg2+ concentration was 0.28±2.75%; p =0.917). Reoxygenation non-significantly increased the Mg2+ levels by 4.27±2.66% (p =0.107). Together, the U-74389G administration and reoxygenation non-significantly increased the Mg2+ levels by 0.36±1.64% (p =0.823).

Conclusion: U-74389G administration, alone or in concert with reoxygenation did not significantly affect Mg2+ level in blood after experimental hypoxia in rats.

Splenectomy is a serious operation, which includes the removal of the largest peripheral immune organ. Vast array of different pools of immunocompetent cells and immune-factors eliminate from the body as a result of this operation. Occurrence of hyposplenism in our country pediatric service is not determined — there are neither clear criteria for its diagnosis, nor approved algorithms for prevention. Data of postsplenectomy sepsis incidence in Russia are unknown. In this review article authors give contemporary literature data relating to the issue of developing hyposplenism and changes in the body after removal of spleen. Systemic effect of organ-resecting operation and the basic directions of overwhelming post-splenectomy infection and sepsis prevention are discussed.

Category A select agents continue to be major threat to human population both as naturally occurring diseases and as potential weapon of bioterrorists. Anthrax and botulism are probably the most threatening agents as both have virtually uncontrolled natural reservoirs from which they can be isolated and propagated. Available specific antitoxin therapy of both diseases is outdated; its efficiency is questionable as well as safety of reactogenic or human-derived components used in treatment. Highly sensitive toxin detection techniques are still not as widespread as it needed for timely alerting medical services. There is urgent need of pre-exposure prophylaxis and postexposure specific antitoxin therapy for anthrax and botulism. Analysis of modern studies in the field suggests oligoclonal antibodies acting against receptor-binding toxin subunits and nucleic acid aptamers as allosteric inhibitors of metlloproteolytic toxin components as the most promising candidates for development of efficient antitoxin therapy

Background: Intramedullary transphyseal elastic osteosynthesis is used in children for the diseases accompanied by the reduced strength properties of bone tissue, and primarily for osteogenesis imperfecta.

Objective: The purpose of the experimental study was to investigate tibial growth under the conditions of transphyseal counter-directed insertion of elastic rods without bone integrity breaking, under transverse fracture modeling, as well as under combining transphyseal reinforcement and subperiosteal positioning the titanium mesh with the elastic rods intervolved in it.

Methods: Non-randomized controlled trial was performed. Three series of experiments performed in 18 puppies. Counter-directed transphyseal reinforcement of tibia performed in Series I,, transphyseal reinforcement combined with transverse osteotomy of leg bones — in Series II, transphyseal elastic osteosynthesis and subperiosteal positioning the titanium nickelide mesh with intervolved in it elastic rods during transverse leg bone osteotomy performed in Series III.

Results: Transphyseal reinforcement resulted in growth retardation of the operated tibia. The loss of residual growth was 3.8 mm (p =0.078) in series I; 7.8 mm (p =0.032) — in series II; 7.7 mm (p =0.042) — in series III. Eccentric insertion of transphyseal rods formed an angular deformity (mean value 7°; p =0.023) of the distal tibial epiphysis in the process of residual growth. Periosteal and endosteal reactions contributed to enlargement of diaphyseal diameter of 3.9 mm (series II; p =0.037) and 3.8 mm (series III; p =0.041). Any difference of diameter between operated and intact tibia was not observed in series I.

Conclusion: Intramedullary transphyseal reinforcement retards longitudinal bone growth. The positioning of the telescopic systems should be as close as possible to the center of growth plates in order to prevent angular deformities. Subperiosteal reinforcement doesn’t retard consolidation of fragments, and it can be combined with intramedullary transphyseal osteosynthesis

Background: The «years of potential life lost» (YPLL) indicator is widely used in international studies. Still YPLL's variety in the Russian regions is poorly investigated.

Objective: Our aim was to study the performance of YPLL in the regions of Russia in 2013 and their relationship with the regional socio-economic indicators.

Methods: Non-clinical observational study was conducted. The object of study — YPLL in the Russian regions. YPLL per 100 thousand population (non-standardized and standardized to the world standard population age structure) is based on data of Rosstat. Subject of investigation — YPLL variability in the regions, the evaluation of the correlation between YPLL, climatic effect and socio-economic indicators of the regions.

Results: The average standardized YPLL in the region was 20 185 (95% CI 19 272–21 029); non-standardized — 25 880 (95% CI 24 744–26 957). Maximum standardized YPLL above the minimum in 3.4 times. Minimum YPLL rates were recorded in the North Caucasus, Moscow and St. Petersburg; maximum — in Chukotka, Republic of Tuva and the Jewish Autonomous Region. Negative statistically significant correlation to the average power detected between YPLL and: 1) specific weight of the total space, equipped with water supply, hot water supply and sanitation; 2) share of population with tertiary education in total population; 3) road density (km of roads per 1000 sq km); 4) climatic conditions.

Conclusion: The regional YPLL indicators vary considerably and are higher than the similar indicators in developed countries. According to the findings PYLL indicator are influenced by economic factor,indicators of household living conditions of the population, the infrastructure system in the region, the level of education of the population and climatic effect. The resuts we got require further multidisciplinary study of the problem.

Objective: Our aim was to assess effect of antiasthenic drug adamantilbromphenylamine on the immune system and symptoms of asthenia in patients with non-psychotic mental disorders and to reveal possible criteria for prediction of treatment efficiency.

Methods: Uncontrolled study with interrupted time series was carried out. According to efficiency of treatment patients were divided into two groups (group 1 (n =21) — very much improved and much improved; group 2 (n =9) — minimally improved). Adamantilbromphenylamine was administered to patients as a monotherapy 100 mg a day for 28 days. Examination was conducted before and after therapy. Severity of asthenic symptoms according to MFI-20 scale was identified; cellular and humoral immunity parameters, mitogen-induced production of interleukins (IL) 1β and IL 4 by immunocompetent cells of patients were assessed.

Results: 30 patients with non-psychotic mental disorders with predominance of asthenic symptomatology in clinical picture of the disease were examined. Before therapy every proband had over 60 points across 5 items of MFI-20 scale. As compared with control decrease of number of lymphocytes of CD3+-, HLA-DR+, CD16+-phenotypes; increase in the ratio of CD4+/CD8+; concentration of serum IgМ; phagocytic activity of neutrophils were revealed. In the end of therapy in group 1, sum total of points of asthenia decreased up to 26 (23–37) (p <0.001); in group 2 — up to 57 (47–61). Only in group 1 positive dynamic of immune parameters was revealed. It was shown that baseline level of proinflammatory cytokine IL 1β in group with apparent therapeutic effect of the drug was reliably lower, than in group with minimal improvement (p =0.005). These differences remained also after course of therapy (p =0.042).

Conclusion: Interrelationship of clinical-immunological effect of adamantilbromphenylamine has been revealed; intensity of production of IL 1β may be considered as a criterion of prognosis of efficiency of treatment with adamantilbromphenylamine in patients with non-psychotic mental disorders

MicroRNAs (miRs) are the class of short nucleotide sequences (21–27 nucleotides) RNA, non-coding protein synthesis. miRs are known as effective posttranscriptional negative regulators of gene expression with specific binding sites of targeted messenger RNA (mRNA) in the cytoplasm, providing translational repression or degradation of the target miR transcript. In this review we studied the role of miRNAs in the development of a physiological pregnancy and obstetric complications. The placenta is a unique organ which provides modulation of the immune system of the maternal organism during pregnancy including miRs which determine immunological tolerance of the body to the tissues of the fetus. Thus the «placental» miRs in maternal circulation may be the potential biomarker revealed at various obstetric pathology on the early stages before clinical manifestation of the diseases.

.

.

Секция профилактической медицины Отделения медицинских наук РАН с прискорбием сообщает, что 20 августа 2015 г. на 96-м году жизни скончалась выдающийся ученый-гигиенист, профессор, доктор медицинских наук, академик РАН Шицкова Анастасия Павловна.