The Effect of the Antioxidant Drug U-74389G on Magnesium Levels During Hypoxia–Reoxygenation Injury in Rats

Cover Page


Objective: The aim of this experimental study was to examine the effect of the antioxidant drug U-74389G in a rat model of hypoxia – reoxygenation using the previously established protocol. Effects of treatments were evaluated by magnesium (Mg2+) levels in blood.

Methods: Nonrandomized controlled study was performed. Mg2+ levels were determined in 60 min (groups A and C) and 120 min (groups B and D) after starting the reoxygenation. Groups A and B received no drugs, whereas rats from groups C and D were administered with U-74389G.

Results: 40 rats 16–18 weeks old of a mean weight of 2312 g were employed in the study. It is demonstrated that U-74389G administration did not alter the Mg2+ levels (decrease in Mg2+ concentration was 0.28±2.75%; p =0.917). Reoxygenation non-significantly increased the Mg2+ levels by 4.27±2.66% (p =0.107). Together, the U-74389G administration and reoxygenation non-significantly increased the Mg2+ levels by 0.36±1.64% (p =0.823).

Conclusion: U-74389G administration, alone or in concert with reoxygenation did not significantly affect Mg2+ level in blood after experimental hypoxia in rats.

About the authors

C. Τsompos

Mesologi County Hospital

Author for correspondence.



C. Panoulis

Aretaieion Hospital, Athens University




K. Τοutouzas

Aretaieion Hospital, Athens University




G. Ζografos

Aretaieion Hospital, Athens University




A. Papalois

Exprerimental Research Center ELPEN Pharmaceuticals, S.A. Inc., Co.




95 Marathonos Avenue, Пикерми, 19009, Аттика


  1. Li C., Jackson R.M. Reactive species mechanisms of cellular hypoxia-reoxygenation injury. Am. J. Physiol. Cell Physiol. 2002; 282 (2): 227–241.
  2. Available at: (accessed: 13.06.2015).
  3. Shi F., Cavitt J., Kenneth L. 21 aminosteroid and 2 (aminomethyl) chromans inhibition of arachidonic acid-induced lipid peroxidation and permeability enhancement in bovine brain microvessel endothelial cell monolayers. Free Radical Biol. Med. 1995; 19 (3): 349–357.
  4. Τsompos C., Panoulis C., Τοutouzas K., Ζografos G., Papalois A. The Effect of the Antioxidant Drug «U-74389G» on Haemoglobin Levels Following a Hypoxemia / Re-oxygenation Protocol in Rats. J. Crit. Care Med. 2015; 1 (3): 102–106.
  5. Tsompos C., Panoulis C., Toutouzas K., Zografos G., Papalois A. The acute effect of the antioxidant drug «U-74389G» on mean corpuscular hemoglobin levels during hypoxia reoxygenation injury in rats. Med. Jad. 2015; 45 (1–2): 17–24.
  6. Siegler J.E., Boehme A.K., Albright K.C., Bdeir S., Kar A.K., Myers L., Beasley T.M., Martin-Schild S. Acute decrease in serum magnesium level after ischemic stroke may not predict decrease in neurologic function. J. Stroke Cerebrovasc. Dis. 2013; 22 (8): 516–521.
  7. Lee K.C., Chang C.Y., Chuang Y.C., Young M.S., Huang C.M., Yin W.H. Сombined St. Thomas and histidine tryptophan ketoglutarat solutions for myocardial preservation in heart transplantation patients. Transplant Proc. 2012; 44 (4): 886–889.
  8. van den Bergh W.M., Albrecht K.W., Berkelbach van der Sprenkel J.W., van Gijn J. Magnesium therapy after aneurysmal subarachnoid haemorrhage a dose finding study for long term treatment. Acta Neurochir (Wien). 2003; 145 (3): 195–199.
  9. Whitelaw A., Thoresen M. Clinical trials of treatments after perinatal asphyxia. Curr. Opin. Pediatr. 2002; 14 (6): 664–668.
  10. Ichiba H., Tamai H., Negishi H., Ueda T., Kim T.J., Sumida Y. Randomized controlled trial of magnesium sulfate infusion for severe birth asphyxia. Pediatr. Int. 2002; 44 (5): 505–509.
  11. Khan IA. Clinical and therapeutic aspects of congenital and acquired long QT syndrome. Am. J. Med. 2002; 112 (1): 58–66.
  12. Hoenicke E.M., Peterseim D.S., Ducko C.T., Sun X., Damiano R.J. Donor heart preservation with the potassium channel opener pinacidil: comparison with University of Wisconsin and St. Thomas’ solution. J. Heart Lung Transplant. 2000; 19 (3): 286–297.
  13. Yano Y., Milam D.F., Alexander J.C. (Jr.). Terminal magnesium cardioplegia: protective effect in the isolated rat heart model using calcium accentuated ischemic damage. J. Surg. Res. 1985; 39 (6): 529–534.
  14. Krause S., Hess M.L. Characterization of cardiac sarcoplasmic reticulum dysfunction during short-term, normothermic, global ischemia. Circ. Res. 1984; 55 (2): 176–184.
  15. Heim C., Kolasiewicz W., Sontag K.H. The effects of the 21 aminosteroid «U-74389G» on spatial orientation in rats after a cerebral oligemic episode and iron-induced oxidative stress. J. Neural. Transm. 2000; 107 (1): 95–104.
  16. Moore R.M., Muir W.W., Bertone A.L., Muir W.W., Stromberg P.C., Beard W.L. Effects of dimethyl sulfoxide, allopurinol, 21 aminosteroid «U-74389G» and manganese chloride on low flow ischemia and reperfusion of the large colon in horses. Am. J. Vet. Res. 1995; 56 (5): 671–687.



Abstract - 542

PDF (Russian) - 184

PDF (English) - 152


Article Metrics

Metrics Loading ...



Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies