Vol 68, No 9 (2013)

The article discusses results of the structural and functional analysis of molecular genetic abnormalities in various malignant tumors. Investigations have discovered more than 20 new markers for sporadic breast cancer. Several of them formed the test system, allowing the diagnosis with a specificity of 100%. Appearance of TMPRSS2/ERG4 chimeric gene is a frequent tumor-specific event, its expression is correlated with more aggressive forms of prostate cancer, may serve as a molecular marker for tumor cells and androgen assessment of tumor response to hormonal therapy. The effective systems for the early diagnosis of cervix and endometrium cancer were developed as well. Mutations in the VHL, deletions of chromosome 3 and methylation of several genes can predict the course and selection of effective therapy of clear cell kidney cancer. a number of molecular markers were identified for early diagnosis and prognosis of recurrence of bladder cancer. For diagnosis, prognosis and treatment of brain tumors we developed an effective complex system of markers. Protocol of molecular genetics investigation reveals the cause of the disease by more than 90% of patients with retinoblastoma. In order to study abnormal methylation in tumor genomes an innovative technology AFLOAT has been developed that allows to efficiently identify new markers with diagnostic value. Test systems of molecular genetic and epigenetic markers for early diagnosis and prognosis as well as for cancer therapy optimization have shown to be effective, have been approved for use in clinical practice and are being introduced into practical healthcare.

Objective: to study apoptosis of individual cellular components of the vascular wall of coronary arteries at different morphological stages of atherosclerosis. Material and methods. The study was performed on coronary arteries taken from 52 deceased patients with atherosclerosis and coronary heart disease at different stages of atherogenesis. For morphological study prepared paraffin sections, which were stained for morphological studies were prepared paraffin sections, which were stained with hematoxylin and eosin, by Van Gieson, Masson, on lipids with Sudan black B, according to Van Cossu. .To determine apoptosis, TUNEL method used in paraffin sections. Apoptotic index (AI) was calculated by TUNEL-positive cells and the average inner shell coronary artery around the perimeter each with increasing microscopic 1000. Results. Investigation showed significant apoptosis (p <0.05) increase in AI smooth muscle, endothelial cells, macrophages in the coronary arteries affected by atherosclerosis compared to intact control group vascular segments significant reduction AI endothelial, smooth muscle cells and macrophages (p  0,05) traced from the early stages of atherogenic disorders to atheromatosis. Conclusions. It is established that apoptosis of smooth muscle cells, macrophages and endothelial cells is the most intensive on early stages of atherosclerotic process. In process of progressing of atherosclerosis intensity and prevalence of apoptosis of coronary artery wall cells decreases, and processes of necrosis becomes predominant. Apoptosis of coronary artery wall cells is valuable in increasing the zones of atheromatosis, plaque destabilizations, and also increases the risk of thrombosis and ulcerations.

Objective: To study the mortality of the male population of working age in the industrial cities depending on the specifics of the town-forming enterprises. Materials and methods.  In three cities in the Murmansk region, where the town -forming enterprises of non-ferrous metallurgy (Monchegorsk, Kandalaksha) and the mining industry (Kirovsk) are located, the capital of the region (Murmansk), and in the country as a whole has been studied of mortality on average for 6-year period  (2000-2005) age-standardized death rates (direct method). Results. Comparative analysis of the causes and death rates in males of working age has shown that in all the studied cities of Murmansk region there were higher death rates in men of working age from diseases of the circulatory system (in 1,3–2,0 times) and diseases of the respiratory system (in 1,1–1,8 times) in comparison with Russia. In industrial cities, compared with Murmansk,  higher death rates of male population of working age from causes which etiologically are connected with harmful factors of manufacture have been registered: from cardiovascular diseases — in 1,1–1,5 times and respiratory diseases — in 1,2–1,6 times. In cities where non-ferrous metallurgy enterprises are located, concerning to cancerogenic, death rates from malignant neoplasms have been raised in 1,2–1,4 times. This happens, in particular, in Monchegorsk where Open Joint-Stock Company «Severonickel Combine» enterprise is located. Mortality from lip, oral cavity and pharynx cancer raised in 1,8 times: the localization specific at influence of nickel.

The purpose of this work was to evaluate the degree of activation of platelets chronically schizophrenic patients with paroxysmal course of the disease during outcome from the attack of disease or in process of forming of the remission. Platelets in platelet-rich-plasma (PRP) and after PRP gel-filtration through the column with Sepharose CL-2B taken from the ulnar vein of males with chronic schizophrenia during outcome from the attack of disease or in process of forming of the remission were measured. Patients were investigated on the peak of the disease attack (visit 1), at the stage of significant improvement of clinical state (visit 2), and at the stage of stable drug-induced remission (visit 3). Platelet quantities in PRP prepared by centrifugation are equal in patients in visits 1, 2 or 3. These platelets quantities are much less than similar platelet quantities in healthy individuals matched in age. The additional decreases of platelet quantities were found after the elution of PRP platelets from the column with Sepharose CL-2B. These additional platelet quantity losses are increased from visit 1 to visit 3. Such losses of cells indicate decrease in their resistance to various influences, their activation in spite of remission development in this group of patients.