THE DISTRIBUTION OF IODINE-125 LABELED ALPHA-FETOPROTEIN IN THE ANIMAL ORGANISM AND ITS ACCUMULATION IN THE TUMOR

Cover Page


Cite item

Full Text

Abstract

The distribution of iodine-125 labeled human alpha-fetoprotein in mice was studied after its intravenous injection. The maximal accumulation of alpha-fetoprotein in different tissues and organs of animals was observed mainly 5 hours after injection. Then the protein was gradually eliminated from the body. In the liver, intestine and blood of intact animals 125I-alpha-fetoprotein persists for at least three days. Accumulation of alpha-fetoprotein in various tissues and organs may determine the different biological effects of this protein. In the mice with transplanted lymphatic leukemia cells P388 the high level of alpha-fetoprotein accumulation was detected in the tumor tissue, reaching 6% of the injected amount per 1 g of tissue. This allows considering the radionuclide-labeled alpha-fetoprotein as a promising medical radionuclide marker for the radiological detection of malignant tumors.

 

About the authors

S. E. Severin

State Health Institution the Moscow Research Institute of Medical Ecology

Author for correspondence.
Email: sergsev@inbox.ru
доктор химических наук, профессор, член-корр. РАМН, генеральный директор ГУЗ Московский НИИ медицинской экологии Адрес: 117149, Москва, Симферопольский бульвар, д. 8 Тел.: (499) 613-23-20 Россия

E. Yu. Moskaleva

State Health Institution the Moscow Research Institute of Medical Ecology

Email: moskalevaey@mail.ru
доктор биологиче-ских наук, профессор, заведующая лабораторией клеточной биохимии ГУЗ Московский НИИ меди-цинской экологии Департамента здравоохранения г. Москвы Адрес: 117149, Москва, Симферопольский бульвар, д. 8 Тел.: 8-916-522-40-54 Россия

V. N. Kulakov

Russian Research Center of Molecular Diagnostics and Treatment

Email: vikov@rambler.ru
доктор химических наук, профессор, ведущий научный сотрудник отдела радиационных технологий Федеральный медицин-ский биофизический центр им. А.И. Бурназяна Федерального медико-биологического агентства Российской Федерации Адрес: 123182, Москва, ул. Живописная, д. 46 Тел.: 8(499) 193-01-86; факс: 8(499) 193-45-73 Россия

E. S. Severin

Federal Medical Biophysical A.I. Burnasyan’s Centre of Federal Biomedical Agency of the Russian Federation

Email: e.severin@mail.ru
доктор химических наук, профессор, член-корр. РАН, генеральный директор ОАО «Всероссийский научный центр молекулярной диагностики и лечения» Адрес: 117149, Москва, Симферопольский бульвар, д. 8 Тел.: (499) 613-23-51 Россия

I. I. Slobodyanik

Russian Research Center of Molecular Diagnostics and Treatment

Email: vikov@rambler.ru
старший инженер отдела радиационных технологий Федерального медицин-ского биофизического центра им. А.И. Бурназяна Федерального медико-биологического агентства Российской Федерации Адрес: 123182, Москва, ул. Живописная, д. 46 Тел.: 8(499) 193-01-86; факс: 8(499) 193-45-73 Россия

T. P. Klimova

Russian Research Center of Molecular Diagnostics and Treatment

Email: tab@ineos.ac.ru
кандидат физико-математических наук, научный сотрудник Института элементоорганических соединений им. А.Н. Несмеянова РАН Адрес: 119991, Москва, ул. Вавилова, д. 28 Тел.: 8(499) 135-92-69 Россия

References

  1. Deutsch H.F. Chemistry and biology of alpha-fetoprotein. Adv. Cancer Res. 1991; 56: 253–312.
  2. Chereshnev V.A., Rodionov S.Yu., Cherkasov V.A., Malyutina N.N., Orlov O.A. Al’fa-fetoprotein. — Ekaterinburg: UrO RAN, 2004. — 376 s.
  3. Bennett J.A., Zhu S., Pagano-Mirarchi A., Kellom T.A., Jacobson H.I. Alpha-fetoprotein derived from a human hepatoma prevents growth of estrogen-dependent human breast cancer xenografts. Clin. Cancer Res. 1998; 4(11): 2877–2884.
  4. Parikh R.R., Gildener-Leapman N., Narendran A. et al. Prevention of N-methyl-N-nitrosourea-induced breast cancer by alphafetoprotein (AFP)-derived peptide, a peptide derived from the active site of AFP. Clin. Cancer Res. 2005; 11(23): 8512–8520.
  5. Suzuki Y., Zeng C.Q., Alpert E. Isolation and partial characterization of a specific alpha-fetoprotein receptor on human monocytes. J. Clin. Invest. 1992; 90(4): 1530–1536.
  6. Torres J.M., Laborda J., Naval J. et al. Expression of alpha-fetoprotein receptors by human T-lymphocytes expressduring blastic transformation. Molec. Immunol. 1989; 57(2): 222–228.
  7. Moro R., Tamaoki Т., Wegmann T.G. et al. Monoclonal antibodies directed against a widespread oncofetal antigen: The Alpha-Fetoprotein Receptor.Tumor Biol. 1993; 14: 116–130.
  8. Nitsvetov M.B., Moskaleva E.Yu., Posypanova G.A. i dr. Izuchenie ekspressii retseptora AFP v opukholevykh i normal’nykh tkanyakh cheloveka s pomoshch’yu immunogistokhimicheskogo metoda. Immunologiya. 2004; 26(2): 122–125.
  9. Uriel J., Villacampa М.J., Моrо R. et al. Uptake of radio labeled AFP by mouse mammary carcinomas and its usefulness in tumor scintigraphy. Cancer Res. 1984; 44: 5314–5319.
  10. Severin S.E., Moskaleva E.Yu., Posypanova G.A. et al. In vivo antitumor activity of cytotoxic drugs conjugated with human α-fetoprotein. Tumor Targeting. 1996; 2: 299–306.
  11. Kulakov V.N., v sb. «Organicheskie soedineniya, mechennye radioaktivnymi izotopami». M., TsNIIAtominform, 1982. — Ch. 2. — 75–84 s.
  12. Mizejewski G.J., Butterstein G. .Survey of functional activities of alpha-fetoprotein derived growth inhibitory peptides: review and prospects. Curr. Protein Pept. Sci. 2006; 7: 73–100.
  13. Terentiev A.A., Moldogazieva N.T. Structural and functional mapping of alpha-fetoprotein. Biochemistry. 2006; 71: 120–132.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2012 "Paediatrician" Publishers LLC



This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies