ETIOLOGICAL STRUCTURE OF ACUTE RESPIRATORY VIRAL INFECTIONS MORBIDITY IN NOVOSIBIRSK AND NOVOSIBIRSK REGION IN EPIDEMIC SEASON 2011–2012

Cover Page


Cite item

Full Text

Abstract

Introduction. ARI occupying the first place in the structure of total human morbidity. The aim of the study was to investigate the species diversity of the viruses causing ARI among residents of the Novosibirsk region during epidemic season (October to April). Materials and methods. 164 nasopharyngeal swabs were collected and analyzed. Viral RNA/DNA, cDNA synthesis and PCR were carried out employing “RIBO-prep”, “Reverta-L”, “AmpliSens Influenza virus A/B-FL” and “AmpliSens ARI-screen-FL” kits (CRI of Epidemiology). Results. Etiological agent of the disease was found in 69 (43%) samples. Monoinfection was found in 58 (35%).In 14 (9%) samples were detected serogroup I coronaviruses, in 13 (8%) rhinoviruses, in 7 (4%) respiratory syncytial virus, in 6 (4%) parainfluenza virus type 1, in 5 (3%) parainfluenza virus type 3. Adenoviruses and bocavirus were identified in 3 (2%) samples. Parainfluenza virus type 2 and 4, metapneumovirus, serogroup II coronaviruses (HKU1 and OC43) were presented in 2 (1%) samples. In 11 (7%) samples was found mixed infection. Conclusion. The majority of common colds were caused by serogroup I coronaviruses (NL63 and 229E), rhinoviruses and mixed infections. The peak of species variability of viruses caused acute respiratory infections was determined in age group of children 2–4 years old. In older age groups the species variability of analyzed viruses was decreased, rhinovirus infection becomes prevalent.

 

About the authors

E. I. Sergeeva

State Research Center of Virology and Biotechnology «Vector», Koltsovo, Novosibirsk Region, Russian Federation

Author for correspondence.
Email: esergeeva@vector.nsc.ru
Junior Research Worker, Department Collection of Microorganisms, State Research Center of Virology and Biotechnology (VECTOR) Address: 630559, Novosibirsk region, Novosibirsk district, Koltsovo work settlement, 12; tel.: (923) 239-58-22 Russian Federation

E. V. Ivanova

Center of Hygiene and Epidemiology in Novosibirsk Region, Novosibirsk, Russian Federation

Email: Virusolog@cn.ru
Head of Virology Laboratory, Centre of Hygiene and Epidemiology in the Novosibirsk Region Address: 630099, Moscow, Frunze St., 84; tel.: (383) 224-26-52 Russian Federation

A. N. Shvalov

State Research Center of Virology and Biotechnology «Vector», Koltsovo, Novosibirsk Region, Russian Federation

Email: shvalov@mail.ru
PhD, Research Worker, Department of Molecular Virology of Flavivirus and Viral Hepatitis, State Research Center of Virology and Biotechnology (VECTOR) Address: 630559, Novosibirsk region, Novosibirsk district, Koltsovo work settlement, 12; tel.: (383) 363-47-60 Russian Federation

V. A. Ternovoi

State Research Center of Virology and Biotechnology «Vector», Koltsovo, Novosibirsk Region, Russian Federation

Email: tern@vector.nsc.ru
PhD, Head of the Laboratory of Molecular Epidemiology of especially dangerous infections, State Research Center of Virology and Biotechnology (VECTOR) Address: 630559, Novosibirsk region, Novosibirsk district, Koltsovo work settlement, 12; tel.: (383) 363-48-25 Russian Federation

V. N. Mikheev

State Research Center of Virology and Biotechnology «Vector», Koltsovo, Novosibirsk Region, Russian Federation

Email: vn_mikheev@vector.nsc.ru
Deputy Director General for Research and epidemiological work, State Research Center of Virology and Biotechnology (VECTOR) Address: 630559, Novosibirsk region, Novosibirsk district, Koltsovo work settlement, 12; tel.: (383) 336-73-27 Russian Federation

A. P. Agafonov

State Research Center of Virology and Biotechnology «Vector», Koltsovo, Novosibirsk Region, Russian Federation

Email: agafonov@vector.nsc.ru
Deputy Director General for Research, State Research Center of Virology and Biotechnology (VECTOR) Address: 630559, Novosibirsk region, Novosibirsk district, Koltsovo work settlement, 12; tel.: (383) 306-15-12 Russian Federation

L. K. Ivanova

Center of Hygiene and Epidemiology in Novosibirsk Region, Novosibirsk, Russian Federation

Email: cgnso@cn.ru
Head Physician of the Center for Hygiene and Epidemiology in the Novosibirsk region Address: 630099, Moscow, Frunze St., 84; tel.: (383)224-24-52 Russian Federation

A. N. Sergeev

State Research Center of Virology and Biotechnology «Vector», Koltsovo, Novosibirsk Region, Russian Federation

Email: esergeeva@vector.nsc.ru
Director General of State Research Center of Virology and Biotechnology (VECTOR) Address: 630559, Novosibirsk region, Novosibirsk district, Koltsovo work settlement, 12; tel.: (383) 336-60-10 Russian Federation

References

  1. Svedeniya ob infektsionnykh i parazitarnykh zabolevaniyakh (Forma 1) za yanvar' – dekabr' 2010 [Information on infectious and parasitic diseases (Form 1) for January - December 2010]. Available at: http://75.rospotrebnadzor.ru/content/svedeniya-ob-infektsionnykh-i-parazitarnykh-zabolevaniyakh-forma-1-za-yanvar-dekabr-2010-god. (accessed: 23.01.13).
  2. Svedeniya ob infektsionnykh i parazitarnykh zabolevaniyakh (Forma 1) za yanvar' – dekabr' 2011 [Information on infectious and parasitic diseases (Form 1) for January - December 2011]. Available at: http://75.rospotrebnadzor.ru/content/svedeniya-ob-infektsionnykh-i-parazitarnykh-zabolevaniyakh-forma-1-za-yanvar-dekabr-2011-god. (accessed: 23.01.13).
  3. Van den Hoogen B.G., De Jong J.C., Groen J., Kuiken T., De Groot R., Fouchier R.A., Osterhaus A.D. A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat. Med. 2001 Jun; 7 (6): 719–724.
  4. Van der Hoek L., Ihorst G., Sure K., Vabret A., Dijkman R., De Vries M., Forster J., Berkhout B., Uberla K. Burden of disease due to human coronavirus NL63 infections and periodicity of infection. J. Clin. Virol. 2010 Jun; 48 (2): 104–108.
  5. Peiris J., Yuen K., Osterhaus A., Stöhr K. The severe acute respiratory syndrome. N. Engl. J. Med. 2003 Dec;349(25):2431–2441.
  6. Allander T., Tammi M., Eriksson M., Bjerkner A., Tiveljung-Lindell A., Andersson B. Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc. Natl. Acad. Sci. U.S.A. 2005 Sep; 102(36): 12891–12896.
  7. Woo P., Lau S., Chu C., Chan K., Tsoi H., Huang Y., Wong B., Poon R., Cai J., Luk W., Poon L., Wong S., Guan Y., Peiris J., Yuen K. Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia. J. Virol. 2005 Jan; 79(2): 884–895.
  8. Peiris J., De Jong M., Guan Y. Avian influenza virus (H5N1): a threat to human health. Clin. Microbiol. Rev. 2007 Apr;20(2):243–267.
  9. Fraser C., Donnelly C., Cauchemez S., Hanage W., Van Kerkhove M., Hollingsworth T., Griffin J., Baggaley R., Jenkins H., Lyons E., Jombart T., Hinsley W., Grassly N., Balloux F., Ghani A., Ferguson N., Rambaut A., Pybus O., Lopez-Gatell H., Alpuche-Aranda C., Chapela I., Zavala E., Guevara D., Checchi F., Garcia E., Hugonnet S., Roth C. Pandemic potential of a strain of influenza A (H1N1): early findings. Science. 2009 Jun; 324 (5934): 1557–1561.
  10. Bermingham A., Chand M., Brown C., Aarons E., Tong C., Langrish C., Hoschler K., Brown K., Galiano M., Myers R., Pebody R., Green H., Boddington N., Gopal R., Price N., Newsholme W., Drosten C., Fouchier R., Zambon M. Severe respiratory illness caused by a novel coronavirus, in a patient transferred to the United Kingdom from the Middle East, September 2012. Euro Surveill. 2012; 17 (40). Available at: http://www.ncbi.nlm.nih.gov/pubmed/23078800
  11. Epidemiologicheskaya situatsiya po grippu v Rossii i mire (2012 g., 53-ya ned) [The epidemiological situation of influenza in Russia and the world (2012, 53rd week)]. Available at: http://www.influenza.spb.ru/en/influenza_surveillance_system_in_russia/epidemic_situation/ (accessed: 12.01.2013).
  12. Andreasen V. Dynamics of annual influenza A epidemics with immuno-selection. J. Math. Biol. 2003 Jun; 46 (6): 504–536.
  13. Atmar R.L., Piedra P.A., Patel S.M., Greenberg S.B., Couch R.B., Glezen W.P. Picornavirus, the most common respiratory virus causing infection among patients of all ages hospitalized with acute respiratory illness. J. Clin. Microbiol. 2012 Feb; 50 (2): 506–508.
  14. Xiang Z., Gonzalez R., Wang Z., Xiao Y., Chen L., Li T., Vernet G., Paranhos-Baccalà G., Jin Q., Wang J. Human rhinoviruses in Chinese adults with acute respiratory tract infection. J. Infect. 2010 Oct;61(4):289–298.
  15. Paranhos-Baccalà G., Komurian-Pradel F., Richard N., Vernet G., Lina B., Floret D. Mixed respiratory virus infections. J. Clin. Virol. 2008 Dec;43(4):407–410.
  16. Allander T., Jartti T., Gupta S., Niesters H., Lehtinen P., Osterback R., Vuorinen T., Waris M., Bjerkner A., Tiveljung-Lindell A., Van den Hoogen B., Hyypiä T., Ruuskanen O. Human bocavirus and acute wheezing in children. Clin. Infect. Dis. 2007 Apr;44(7):904–910.
  17. Naghipour M., Cuevas L., Bakhshinejad T., Dove W., Hart C.. Human bocavirus in Iranian children with acute respiratory infections. J. Med. Virol. 2007 May;79(5):539–543.
  18. Fry A., Lu X., Chittaganpitch M., Peret T., Fischer J., Dowell S., Anderson L., Erdman D., Olsen S.. Human bocavirus: a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in Thailand. J. Infect. Dis. 2007 Apr; 195 (7): 1038–1045.
  19. Pyrc K., Berkhout B., Van der Hoek L. The novel human coronaviruses NL63 and HKU1. J. Virol. 2007 Apr;81(7):3051–3057.
  20. Lu R., Yu X., Wang W., Duan X., Zhang L., Zhou W., Xu J., Xu L., Hu Q., Lu J., Ruan L., Wang Z., Tan W. Characterization of human coronavirus etiology in Chinese adults with acute upper respiratory tract infection by real-time Rt-PCR assays. PLoS ONE 2012;7(6):e38638.
  21. Ren L., Gonzalez R., Xie Z., Xiong Z., Liu C., Xiang Z., Xiao Y., Li Y., Zhou H., Li J., Yang Q., Zhang J., Chen L., Wang W., Vernet G., Paranhos-Baccalà G., Shen K., Wang J. Human parainfluenza virus type 4 infection in Chinese children with lower respiratory tract infections: a comparison study. J. Clin. Virol. 2011 Jul;51(3):209–212.
  22. Falsey A.R., Walsh E.E. Respiratory syncytial virus infection in adults. Clin. Microbiol. Rev. 2000 Jul;13(3):371–384.
  23. Barati M., Noorbakhsh S., Tabatabaei A., Taj F., Talebi-Taher M. Adenovirus, Influenza Virus A, B and Respiratory Syncitial Virus Infection in Children. International Journal of Infectious Diseases 2008;12, Suppl. 1(0):e66.
  24. Wong S., Pabbaraju K., Pang X., Lee B., Fox J. Detection of a broad range of human adenoviruses in respiratory tract samples using a sensitive multiplex real-time PCR assay. J. Med. Virol. 2008 May;80(5):856–865.
  25. Castro-Rodriguez J., Daszenies C., Garcia M., Meyer R., Gonzales R. Adenovirus pneumonia in infants and factors for developing bronchiolitis obliterans: a 5-year follow-up. Pediatr. Pulmonol. 2006 Oct; 41 (10): 947–953.
  26. Li J., Wang Z., Gonzalez R., Xiao Y., Zhou H., Zhang J., Paranhos-Baccala G., Vernet G., Jin Q., Wang J., Hung T. Prevalence of human metapneumovirus in adults with acute respiratory tract infection in Beijing, China. J. Infect. 2012 Jan; 64 (1): 96–103.
  27. Williams J.V., Harris P.A., Tollefson S.J., Halburnt-Rush L.L., Pingsterhaus J.M., Edwards K.M., Wright P.F., Crowe J. Jr. Human metapneumovirus and lower respiratory tract disease in otherwise healthy infants and children. N. Engl. J. Med. 2004 Jan; 350 (5): 443–450.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 1970 "Paediatrician" Publishers LLC



This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies