RECENT ADVANCES IN THE STUDY OF THE STEM CELLS MIGRATION METHODS

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Abstract

Review is devoted to a comparative analysis of stem cells migration methods for studying in experimental conditions and clinics, assessing their strengths and weaknesses. It is impossible to single out any one preferred method for studying the migration of stem cells. Each method has its own set of advantages and disadvantages. X-ray methods " suffer" from non-specific binding of contrast marks, followed by rapid degradation and the release of the body. If it is necessary to study the migration of the transplanted stem cells for a long time interval, the visualization of the reporter gene is the best available techniques, since it can provide a non-invasive real time monitoring of the location in the tissue and stem cells isolated help assess their viability and proliferation. The most attractive of the agents for monitoring - endogenous, i.e. natural constituents stem cells, for example, genetic markers. Criteria for the application of methods to study migration in clinical and experimental results have significant differences. The main criteria for the methods of the study of cell migration in clinical settings, it must be biocompatible, safe, non-toxic, non-invasive. Accordingly, the main criteria for the experimental methods for studying the migration will be accuracy, the ability to quantify the long-term migration of cells and stability of the "label".

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About the authors

A. F. Poveshchenko

Scientific Institution of Clinical and Experimental Lymphology of the Siberian Branch, RAMS, Novosibirsk, Russian Federation

Author for correspondence.
Email: poveshchenkoa200@mail.ru
PhD, Head of the laboratory of protective system physiology of the Federal State Budgetary Institution «Scientific Research Institute of clinical and experimental lymphology» under the Siberian Branch of RAMS. Address: 2, Timakova St., Novosibirsk, 630117; tel.: (383) 333-64-09 Russian Federation

O. V. Poveshchenko

Scientific Institution of Clinical and Experimental Lymphology of the Siberian Branch, RAMS, Novosibirsk, Russian Federation

Email: poveshchenkoa200@mail.ru
MD, Head of the lymphotropic therapy and lymphodiagnostic laboratory of the Federal State Budgetary Institution «Scientific Research Institute of clinical and experimental lymphology» under the Siberian Branch of RAMS. Address: 2, Timakova St., Novosibirsk, 630117; tel.: (383) 333-64-09 Russian Federation

V. I. Konenkov

Scientific Institution of Clinical and Experimental Lymphology of the Siberian Branch, RAMS, Novosibirsk, Russian Federation

Email: lymphology@soramn.ru
PhD, professor, member of the RAMS, director of the Federal State Budgetary Institution «Scientific Research Institute of clinical and experimental lymphology» under the Siberian Branch of RAMS. Address: 2, Timakova St., Novosibirsk, 630117; tel.: (383) 333-64-09 Russian Federation

References

  1. Till J.E., McCulloch E.A. A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radist. Res. 1961; 14 (2):213–222.
  2. Petrov R.V., Khaitov R.M. Migration of stem cells from the bone marrow shielded with uneven exposure. Radiobiologiya = Radiobiology. 1972; 12(1): 69–76.
  3. Frangioni J.V., Hajjar R.J. In vivo tracking of stem cells for clinical trials in cardiovascular disease. Circulation. 2004; 110: 3378–3384.
  4. Torrente, Y., Gavina, M., Belicchi, M., Fiori F, Komlev V., Bresolin N., Rustichelli F. X-ray microtomography for three-dimensional visualization of human stem cell muscle homing. FEBS Lett. 2006; 580(24): 5759–5764.
  5. Detante, O., Moisan A., Dimastromatteo J.,Richard M.J., Riou L., Grillon E., Barbier E., Desruet M.D., DeFraipont F, Segebarth C., Jaillard A., Hommel M., Ghezzi C., Remy C. Intravenous administration of 99mTc-HMPAO-labeled human mesenchymal stem cells after stroke: in vivo imaging and biodistribution. Cell Transplant. 2009; 18 (12): 1369–1379.
  6. Gholamrezanezhad, A., Mirpour S., Bagheri M., Mohamadnejad M., Alimoghaddam K., Abdolahzadeh L., Saghari M., Malekzadeh R. .In vivo tracking of 111In-oxine labeled mesenchymal stem cells following infusion in patients with advanced cirrhosis. Nucl. Med. & Biol. 2011; 38 (7): 961–967.
  7. Kang W.J., Kang H.J., Kim H.S.,Chung J.K.,Lee M.C., Lee D.S. Tissue distribution of 18F-FDG-labeled peripheral hematopoietic stem cells after intracoronary administration in patients with myocardial infarction. J. Nucl. Med. 2006; 47 (8): 1295–1301.
  8. Ramot Y., Steiner M., Morad V., Leibovitch S., Amouyal N., Cesta M.F., Nyska A. et al. Pulmonary thrombosis in the mouse following intravenous administration of quantum dot-labeled mesenchymal cells. Nanotoxicology. 2010; 4(1): 98–105.
  9. Michalet X., Pinaud F.F., Bentolila L.A., Tsay JM, Doose S, Li JJ, Sundaresan G, Wu AM, Gambhir SS, Weiss S. Quantum dots for live cells, in vivo imaging, and diagnostics. Science. 2005; 307(5709): 538–544.
  10. Shah B.S. Clark P.A. Moioli, E.K., Stroscio MA, Mao JJ. Labeling of mesenchymal stem cells by bioconjugated quantum dots. Nano Lett. 2007; 7(10): 3071–3079.
  11. Slotkin J.R., Chakrabarti, L., Dai, H.N. Carney RS, Hirata T, Bregman BS, Gallicano GI, Corbin JG, Haydar TF.. In vivo quantum dot labeling of mammalian stem and progenitor cells. Dev. Dyn. 2007; 236(12): 3393–3401.
  12. Gera A., Steinberg G.K., Guzman R. In vivo neural stem cell imaging: current modalities and future directions. Regenerative Medicine. 2010; 5 (1): 73–86.
  13. Villa C., Erratico S., Razini P., Fiori F., Rustichelli F., Torrente Y., Belicchi M. Stem cell tracking by nanotechnologies. Int. J. Mol. Sci. 2010; 11(3): 1070–1081.
  14. Kustermann E., Roell W., Breitbach M., Wecker S, Wiedermann D., Buehrle C., Welz A., Hescheler J., Fleischmann BK, Hoehn M. Stem cell implantation in ischemic mouse heart: a high-resolution magnetic resonance imaging investigation. NMR Biomed. 2005; 18(6): 362–370.
  15. Walczak P., Zhang J., Gilad A.A., Kedziorek D.A., Ruiz-Cabello J., Young R.G., Pittenger M.F., van Zijl P.C., Huang J., Bulte J.W. Dual-modality monitoring of targeted intraarterial delivery of mesenchymal stem cells after transient ischemia. Stroke. 2008; 39(5): 1569–1574.
  16. Himmelreich U., Hoehn M. Stem cell labeling for magnetic resonance imaging. Minim. Invasive Ther. Allied Technol. 2008; 17: 132–142.
  17. Kraitchman D.L., Bulte J.W. In vivo imaging of stem cells and Beta cells using direct cell labeling and reporter gene methods. Arterioscler. Thromb. Vasc. Biol. 2009; 29 (7): 1025–1030.
  18. Modo M., Cash, D., Mellodew K. Williams SC, Fraser SE, Meade TJ, Price J, Hodges H. Tracking transplanted stem cell migration using bifunctional, contrast agent-enhanced, magnetic resonance imaging. Neuroimage. 2002; 17(2): 803–811.
  19. Higuchi T., Anton M., Dumler K., Seidl S, Pelisek J, Saraste A, Welling A, Hofmann F, Oostendorp RA, Gansbacher B, Nekolla SG, Bengel FM, Botnar RM, Schwaiger M. Combined reporter gene PET and iron oxide MRI for monitoring survival and localization of transplanted cells in the rat heart. J. Nucl. Med. 2009; 50 (7): 1088–1094.
  20. Kasinskaya N.V., Stepanova O.I., Karkishchenko N.N., Karkishchenko V.N., Semenov Kh.Kh., Beskova T.B., Kapanadze G.D., Revyakin A.O., Dengina S.E. Green protein gene as a marker in the transplantation of stem and progenitor cells in the bone marrow. Biomeditsina = Biomedicine. 2011; 2: 30–34.
  21. Reumers V., Deroose C.M., Krylyshkina O., Nuyts J, Geraerts M, Mortelmans L, Gijsbers R, Van den Haute C, Debyser Z, Baekelandt V.Noninvasive and quantitative monitoring of adult neuronal stem cell migration in mouse brain using bioluminescence imaging. Stem Cells. 2008; 26 (9): 2382–2390.
  22. Zhang H.L., Qiao H., Bakken A., Gao F, Huang B, Liu YY, El-Deiry W, Ferrari VA, Zhou R. Utility of dual-modality bioluminescence and MRI in monitoring stem cell survival and impact on post myocardial infarct. Remodeling Acad. Radiol. 2011; 18 (1): 3–12.
  23. Zhang S.J., Wu J.C. Comparison of imaging techniques for tracking cardiac stem cell therapy. J. Nucl. Med. 2007; 48 (12): 1916–1919.
  24. Polzer H., Haaster F.S., Prall W.C., Saller MM, Volkmer E, Drosse I, Mutschler W, Schieker M. Quantification of fluorescence intensity of labeled human mesenchymal stem cells and cell counting of unlabeled cells in phase-contrast imaging: An open-source-based algorithm. Tis. Engineering Part C Meth. 2010; 16 (6); 1277–1285.
  25. Sung C.K., Hong K.A., Lin S. Lee Y, Cha J, Lee JK, Hong CP, Han BS, Jung SI, Kim SH, Yoon KS. Dual-modal nanoprobes for imaging of mesenchymal stem cell transplant by MRI and fluorescence imaging. Korean J. Radiol. 2009; 10 (6): 613–622.

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