RECENT ADVANCES IN THE STUDY OF THE STEM CELLS MIGRATION METHODS

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Abstract

Review is devoted to a comparative analysis of stem cells migration methods for studying in experimental conditions and clinics, assessing their strengths and weaknesses. It is impossible to single out any one preferred method for studying the migration of stem cells. Each method has its own set of advantages and disadvantages. X-ray methods " suffer" from non-specific binding of contrast marks, followed by rapid degradation and the release of the body. If it is necessary to study the migration of the transplanted stem cells for a long time interval, the visualization of the reporter gene is the best available techniques, since it can provide a non-invasive real time monitoring of the location in the tissue and stem cells isolated help assess their viability and proliferation. The most attractive of the agents for monitoring - endogenous, i.e. natural constituents stem cells, for example, genetic markers. Criteria for the application of methods to study migration in clinical and experimental results have significant differences. The main criteria for the methods of the study of cell migration in clinical settings, it must be biocompatible, safe, non-toxic, non-invasive. Accordingly, the main criteria for the experimental methods for studying the migration will be accuracy, the ability to quantify the long-term migration of cells and stability of the "label".

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About the authors

A. F. Poveshchenko

Scientific Institution of Clinical and Experimental Lymphology of the Siberian Branch, RAMS, Novosibirsk, Russian Federation

Author for correspondence.
Email: poveshchenkoa200@mail.ru
PhD, Head of the laboratory of protective system physiology of the Federal State Budgetary Institution «Scientific Research Institute of clinical and experimental lymphology» under the Siberian Branch of RAMS. Address: 2, Timakova St., Novosibirsk, 630117; tel.: (383) 333-64-09 Россия

O. V. Poveshchenko

Scientific Institution of Clinical and Experimental Lymphology of the Siberian Branch, RAMS, Novosibirsk, Russian Federation

Email: poveshchenkoa200@mail.ru
MD, Head of the lymphotropic therapy and lymphodiagnostic laboratory of the Federal State Budgetary Institution «Scientific Research Institute of clinical and experimental lymphology» under the Siberian Branch of RAMS. Address: 2, Timakova St., Novosibirsk, 630117; tel.: (383) 333-64-09 Россия

V. I. Konenkov

Scientific Institution of Clinical and Experimental Lymphology of the Siberian Branch, RAMS, Novosibirsk, Russian Federation

Email: lymphology@soramn.ru
PhD, professor, member of the RAMS, director of the Federal State Budgetary Institution «Scientific Research Institute of clinical and experimental lymphology» under the Siberian Branch of RAMS. Address: 2, Timakova St., Novosibirsk, 630117; tel.: (383) 333-64-09 Россия

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