SOLUBLE CD25 AND CD95 MOLECULES LEVEL AT BURNS

Cover Page
  • Authors: Lebedev M.J.1, Sholkina M.N.1, Novikov D.V.2, Shumilova S.V.2, Novikov V.V.2, Karaulov A.V.2,3
  • Affiliations:
    1. Volga Federal Medical Research Center
    2. N.I. Lobachevskiy National Research Nizhny Novgorod State University
    3. I.M. Sechenov Moscow State Medical University
  • Issue: Vol 72, No 4 (2017)
  • Pages: 276-281
  • Section: РATHOPHYSIOLOGY: CURRENT ISSUES
  • URL: https://vestnikramn.spr-journal.ru/jour/article/view/772
  • DOI: https://doi.org/10.15690/vramn772

Abstract


Background: Burn injury is accompanied by modulation of the many components of immunity, including the system regulation, which includes soluble forms of leukocyte differentiation molecules. Earlier in burn patients, we detected changes in serum levels of soluble differentiation molecules CD25 (sCD25) and CD95 (sCD25). Despite the existence of data on change of serum level of the soluble molecules CD25 and CD95 in the blood of patients with a burn trauma, there are no data on particular cell producers.

Aims: To conduct the analysis of serum level of the molecules sCD25 and sCD95 in the blood of patients with acute burn trauma in comparison with peripheral blood cells composition to obtain data on the types of cells that produce the molecules sCD25 and sCD95.

Materials and methods: Blood samples from 24 heavily burnt patients aged 16 to 77 years were studied. Determination of sCD25 and sCD95 molecules serum levels was performed by ELISA. Number of CD45+CD25+ lymphocytes, CD45+CD95+ cells, CD14+CD95+ monocytes, CD16b+CD95+ neutrophils, and RFMI (relative mean fluorescence intensity) was evaluated by flow cytometry.

Results: In the first five days of the date of burn sCD25 and sCD95 serum levels tended to increase. sCD25 molecules contents in the blood of surviving and dead patients did not depend on the relative content of CD45+CD25+ lymphocytes, RFMI index, but correlated with the absolute level of lymphocytes and leukocytes. Serum levels of sCD95 molecules showed the dependence on the absolute neutrophil count and leukocytes in the survivors and on the absolute content of lymphocytes, neutrophils, and leukocytes in patients who died.

Conclusions: The findings suggest that the lymphocytes in the early period of burn disease are the main cells-producers of sCD25 and affect the increase of its content in the blood serum not due to changes in the density of CD25 molecules expression on their membrane followed by increased shedding but by increasing the number of CD25 positive cells. The main cells-producers of sCD95 molecules for survivors in the early period of burn disease are likely to be the neutrophils and lymphocytes; in the dead patients, the main producers are neutrophils.


Keywords



M. Ju. Lebedev

Volga Federal Medical Research Center

Author for correspondence.
Email: miklgito@mail.ru
ORCID iD: 0000-0001-5596-2619

Russian Federation  

Кандидат медицинских наук, старший научный сотрудник группы иммунологии.

603155, Нижний Новгород, В. Волжская набережная, д. 18/1, тел.: +7 (951) 908-13-42.

SPIN-код: 6801-2854

M. N. Sholkina

Volga Federal Medical Research Center

Email: msholkina@inbox.ru
ORCID iD: 0000-0003-0258-3847

Russian Federation

Кандидат медицинских наук, врач клинической лабораторной диагностики клинико-иммунологической лаборатории.

603155, Нижний Новгород, В. Волжская набережная, д. 18/1, тел.: +7 (831) 436-62-40.

SPIN-код: 9563-6356

D. V. Novikov

N.I. Lobachevskiy National Research Nizhny Novgorod State University

Email: novikov.dv75@mail.ru
ORCID iD: 0000-0001-7049-6935

Russian Federation

Кандидат биологических наук, ведущий научный сотрудник Центра молекулярной биологии и биомедицины.

603950, Нижний Новгород, пр. Гагарина, д. 23, тел./факс: +7 (831) 419-90-64.

SPIN-код: 6801-1613

S. V. Shumilova

N.I. Lobachevskiy National Research Nizhny Novgorod State University

Email: swetlana.shumilova@gmail.com
ORCID iD: 0000-0002-2727-2888

Russian Federation

Кандидат биологических наук, старший научный сотрудник Центра молекулярной биологии и биомедицины.

603950, Нижний Новгород, пр. Гагарина, д. 23, тел./факс: +7 (831) 419-90-64.

SPIN-код: 9562-9450

V. V. Novikov

N.I. Lobachevskiy National Research Nizhny Novgorod State University

Email: mbre@mail.ru
ORCID iD: 0000-0002-2449-7213

Russian Federation

Доктор биологических наук, профессор, заведующий кафедрой молекулярной биологии и биомедицины.

603950, Нижний Новгород, ГСП-20, пр. Гагарина, д. 23, тел./факс: +7 (831) 462-32-16.

SPIN-код: 5492-7871

A. V. Karaulov

N.I. Lobachevskiy National Research Nizhny Novgorod State University; I.M. Sechenov Moscow State Medical University

Email: drkaraulov@mail.ru
ORCID iD: 0000-0002-1930-5424

Russian Federation

Доктор медицинских наук, профессор, академик РАН, заведующий кафедрой клинической иммунологии и аллергологии ПМГМУ им. И.М. Сеченова

119991, Москва, ул. Трубецкая, д. 8, стр. 2, тел.: +7 (495) 635-13-73.

SPIN-код: 4122-5565

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