Comparative Genotyping of Staphylococcus aureus Strains Isolated from Skin Lesions, Nasal Cavities, and Feces of Children with Atopic Dermatitis

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  • Authors: Pikina A.P.1, Shkoporov A.N.1, Kulagina E.V.2, Khokhlova E.V.1, Chaplin A.V.1, Volodin N.N.3, Kafarskaya L.I.1, Korotkiy N.G.1, Efimov B.A.1
  • Affiliations:
    1. Russian National Research Medical University
    2. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
    3. D. Rogachev Federal Research Center of Pediatric Hematology, Oncology and Immunology
  • Issue: Vol 71, No 5 (2016)
  • Pages: 367-374
  • Section: MICROBIOLOGY: CURRENT ISSUES
  • URL: https://vestnikramn.spr-journal.ru/jour/article/view/695
  • DOI: https://doi.org/10.15690/vramn695
  • Cite item

Abstract


Background: The lesion of skin of the majority atopic dermatitis patients is chronically colonized by bacteria belonging to the species Staphylococcus aureus. Topical antibacterial and anti-inflammatory therapy treatment are often ineffective due to fast recolonization by S. aureus and exacerbation of allergic process.

Aims: Our aim was to determine a frequency of S. aureus colonization in skin lesions, mucous membranes of the nasal cavity and intestine of children with atopic dermatitis, to compare the genotypes of Staphylococcus aureus strains isolated from different biotopes of atopic dermatitis patients, and to clarify whether the intestinal and nasal cavities microbiota may act as a source of S. aureus recolonization of skin lesions.

Materials and methods: Bacteriological examination of fecal samples, skin, and nasal swabs was conducted in 38 atopic dermatitis patients. The pure bacterial cultures of S. aureus were identified using API Staph (Biomerieux, France) and Vitek 2 MS (Biomerieux, France). Isolates of S. aureus were subjected to genotyping by analysis of rRNA internal 16S-23S rRNA spacer regions and high resolution melting analysis (HMR) of polymorphic spa X-regions.

Results: 99% S. aureus strains were successfully identified using MALDI-TOF mass-spectrometry. S. aureus cultures were isolated from all biotopes in 31,6% of children, from skin and nasal cavities — in 42% of cases, from skin and feces — in 2,6% of cases, only from skin — in 10,5%, from nasal cavities and feces — in 2,6%, and only from nasal cavities — in 2,6% of cases. In 8% of children, S. aureus was not detected in any of the biotopes. Genotyping of the isolates enabled the detection of 17 different genotypes. A match between the genotypes of skin and nasal strains, and skin and fecal strains was observed in 88% and 61% of the cases respectively.

Conclusions: The observed a high-frequency matching genotypes suggests the possibility of migration of S. aureus strains inside biotopes in humans and the absence of specialization to colonization of any of the niches.


A. P. Pikina

Russian National Research Medical University

Author for correspondence.
Email: apikina@mail.ru
ORCID iD: 0000-0001-7232-3288

Russian Federation Moscow

A. N. Shkoporov

Russian National Research Medical University

Email: a.shkoporov@gmail.com
Moscow

E. V. Kulagina

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: elenka176@yandex.ru
ORCID iD: 0000-0002-5381-1785
Moscow

E. V. Khokhlova

Russian National Research Medical University

Email: a.shkoporov@gmail.com
Moscow

A. V. Chaplin

Russian National Research Medical University

Email: okolomedik@gmail.com
ORCID iD: 0000-0003-1377-7153
Moscow

N. N. Volodin

D. Rogachev Federal Research Center of Pediatric Hematology, Oncology and Immunology

Email: info@fnkc.ru
Moscow

L. I. Kafarskaya

Russian National Research Medical University

Email: likmed@mail.ru
Moscow

N. G. Korotkiy

Russian National Research Medical University

Email: korotkiy_ng@rsmu.ru
Moscow

B. A. Efimov

Russian National Research Medical University

Email: efimov_ba@mail.ru
Moscow

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