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Аптамеры для терапии бактериальных инфекций: проблемы и перспективы

https://doi.org/10.15690/vramn591

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Аннотация

Аптамеры ― короткие одноцепочечные фрагменты нуклеиновых кислот, которые в процессе направленной химической «эволюции в пробирке» на основе технологии SELEX отбирают на специфичность к избранной молекулярной мишени. Возможность получать при помощи SELEX олигонуклеотиды, связывающие широчайший спектр высоко- и низкомолекулярных лигандов, а также простота автоматизированного синтеза привели к созданию широкого спектра приложений аптамеров ― от биосенсоров до противоопухолевых препаратов. С точки зрения медицинской химии, аптамеры являются новым классом молекул, на основе которых могут быть разработаны лекарственные препараты. Поэтому, а также ввиду стабильности, относительной простоты синтеза и создания все более эффективных стратегий селекции мишеньспецифических молекул аптамеры привлекают внимание разработчиков лекарственных средств, в том числе и антибактериальных. Интерес к антибактериальным аптамерам усиливается и в связи с проблемами, возникшими при разработке принципиально новых антибактериальных средств на основе классических химических соединений ― как малых органических молекул, так и синтетических модификаций известных антибиотиков. В настоящем обзоре рассматриваются работы, направленные на создание противоинфекционных аптамеров, и обсуждаются как потенциал, так и существующие на данном этапе ограничения, свойственные этому классу терапевтических молекул.

Об авторах

Н. А. Зенинская
Государственный научный центр прикладной микробиологии и биотехнологии
Россия

младший научный сотрудник лаборатории молекулярной биологии,

142279, Московская обл., Серпуховский р-н, пос. Оболенск



А. В. Колесников
Государственный научный центр прикладной микробиологии и биотехнологии; Институт инженерной иммунологии
Россия

кандидат биологических наук, ведущий научный сотрудник лаборатории молекулярной биологии,

142279, Московская обл., Серпуховский р-н, пос. Оболенск;

заведующий лабораторией иммунопротеомики микроорганизмов,

Любучаны



А. К. Рябко
Государственный научный центр прикладной микробиологии и биотехнологии
Россия

научный сотрудник лаборатории молекулярной биологии,

142279, Московская обл., Серпуховский р-н, пос. Оболенск



И. Г. Шемякин
Государственный научный центр прикладной микробиологии и биотехнологии
Россия

доктор биологических наук, профессор, заместитель директора по научной работе, заведующий лабораторией молекулярной биологии,

142279, Московская обл., Серпуховский р-н, пос. Оболенск



И. А. Дятлов
Государственный научный центр прикладной микробиологии и биотехнологии

член-корреспондент РАМН, доктор медицинских наук, профессор, директор,

142279, Московская обл., Серпуховский р-н, пос. Оболенск



А. В. Козырь
Государственный научный центр прикладной микробиологии и биотехнологии
Россия

кандидат биологических наук, ведущий научный сотрудник лаборатории молекулярной биологии,

142279, Московская обл., Серпуховский р-н, пос. Оболенск



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Для цитирования:


Зенинская Н.А., Колесников А.В., Рябко А.К., Шемякин И.Г., Дятлов И.А., Козырь А.В. Аптамеры для терапии бактериальных инфекций: проблемы и перспективы. Вестник Российской академии медицинских наук. 2016;71(5):350-358. https://doi.org/10.15690/vramn591

For citation:


Zeninskaya N.A., Kolesnikov A.V., Ryabko A.K., Shemyakin I.G., Dyatlov I.A., Kozyr A.V. Aptamers in the Treatment of Bacterial Infections: Problems and Prospects. Annals of the Russian academy of medical sciences. 2016;71(5):350-358. (In Russ.) https://doi.org/10.15690/vramn591

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