About Minimization of Expenses on Allergy Diagnosis in Children: Analysis of Consistency of in Vitro- and in Vivo-Allergic Examinations Results

Cover Page
  • Authors: Snovskaya M.A.1, Batyrova A.S.1, Namazova-Baranova L.S.1,2,3, Alekseeva A.A.1,2, Vishneva E.A.1,2, Kozhevnikova O.V.1, Marushina A.A.1, Lubov V.N.1
  • Affiliations:
    1. Scientific Centre of Children Health
    2. Sechenov First Moscow State Medical University
    3. The Russian National Research Medical University named after N.I. Pirogov
  • Issue: Vol 70, No 6 (2015)
  • Pages: 748-755
  • Section: HEALTH CARE MANAGEMENT
  • URL: https://vestnikramn.spr-journal.ru/jour/article/view/583
  • DOI: https://doi.org/10.15690/vramn583
  • Cite item

Abstract


High morbidity rate of atopic diseases among children, including high importance of grass pollen as a sensitizing agent, determine the relevance of studies on diagnostic examination systems for appointment of adequate therapy. The research of the most relevant allergens for patients to exclude of duplicating and uninformative tests became urgent after development of a new type of diagnostic tests that does not require expensive equipment. 

The objective of this research was to evaluate the results of in vitro- and in vivo-diagnostic examinations of children with various forms of atopic disease caused by pollen of meadow grasses, and to choose the most significant prognostic parameters for the diagnosis. 

Methods: 277 children aged 4–16 years with various forms of atopic disease were included in the study. There were performed skin prick tests and determination of IgE-antibodies levels to allergen extracts of cocksfoot (g3), meadow fescue (g4), timothy grass (g6). 

Results: In the studied group of patients 32–50% of children have antibodies to grass allergens. There was a close correlation of antibody response on the investigated allergens, quantitative coincidence of IgE-antibodies to g3 and g4 allergens levels. IgE (g6) concentration was close to the IgE(g3) and IgE(g4) levels (85,0±21,6%). Analysis of the skin tests results showed that 44% of patients have a positive response to grass allergens, and in vivo-tests results coincide with serological tests results, mostly in a qualitative sense. The most significant relationship was noted between in vivo and in vitro-tests in the results of testing the response to meadow fescue pollen.

Conclusion: Based on these data IgE concentration index to meadow fescue allergens can be used as a prognostic marker to determine the sensitization of patients with different nosology forms of allergy and can help to improve allergic diagnostics.


Marina Andreevna Snovskaya

Scientific Centre of Children Health

Author for correspondence.
Email: snows@inbox.ru

Russian Federation MD

Anna Sergeevna Batyrova

Scientific Centre of Children Health

Email: melograno8@yandex.ru

Russian Federation MD

Leyla Seymurovna Namazova-Baranova

Scientific Centre of Children Health; Sechenov First Moscow State Medical University; The Russian National Research Medical University named after N.I. Pirogov

Email: namazova@nczd.ru

Russian Federation MD, PhD, Professor, academician of Russian academy of sciences

Anna Aleksandrovna Alekseeva

Scientific Centre of Children Health; Sechenov First Moscow State Medical University

Email: aleksaa06@yandex.ru

Russian Federation MD, PhD

Elena Aleksandrovna Vishneva

Scientific Centre of Children Health; Sechenov First Moscow State Medical University

Email: vishneva@nczd.ru

Russian Federation MD, PhD

Ol'ga Viktorovna Kozhevnikova

Scientific Centre of Children Health

Email: fd@nczd.ru

Russian Federation MD, PhD

Anastasiya Aleksandrovna Marushina

Scientific Centre of Children Health

Email: jasnoe@mail.ru

Russian Federation MD

Vasiliy Nikolaevich Lubov

Scientific Centre of Children Health

Email: fake@neicon.ru

Russian Federation MD, resident

  1. Намазова-Баранова ЛС. Аллергия у детей: от теории – к практике. М.: Союз педиатров России. 2011. 668 с.
  2. Ковальчук Л.В. Ганковская Л.В., Мешкова Р.Я. Клиническая иммунология и аллергология с основами общей иммунологии: учебник. М.: ГЭОТАР-Медиа. 2011. 640 с.
  3. Козулина ИЕ, Курбачева ОМ, Ильина НИ. Аллергия сегодня. Анализ новых эпидемиологических данных. Российский аллергологический журнал. 2014;3:3–10.
  4. Bartra J, Sastre J, del Cuvillo A, Montoro J, Jauregui I, Davila I, et al. From pollinosis to digestive allergy. J Investig Allergol Clin Immunol. 2009;19 (Suppl 1):3–10.
  5. Bousquet J, Gern JE, Martinez FD, Anto JM, Johnson CC, Holt PG, et al. Birth cohorts in asthma and allergic diseases: report of a NIAID/NHLBI/MeDALL joint workshop. J Allergy Clin Immunol. 2014;133(6):1535–1546. doi: 10.1016/j.jaci.2014.01.018.
  6. Лопатин АС, Чучуева НД. Эпидемиология аллергического ринита в России и в мире. Российский аллергологический журнал. 2013;2:3–11.
  7. Peternel R, Toth I, Hercog P, Vojnikovic B, Cop R, Bradic-Hammoud M. Influence of aeroallergens on the incidence of conjunctivitis in Zagreb and Zagreb County. Coll Antropol. 2013;37 (Suppl 1):13–17.
  8. Canova C, Heinrich J, Anto JM, Leynaert B, Smith M, Kuenzli N, et al. The influence of sensitisation to pollens and moulds on seasonal variations in asthma attacks. Eur Respir J. 2013;42(4):935–945. doi: 10.1183/09031936.00097412.
  9. Schenk S, Breiteneder H, Susani M, Najafian N, Laffer S, Duchene M, et al. T cell epitopes of Phl p 1, major pollen allergen of timothy grass (Phleum pratense). Crossreactivity with group I allergens of different grasses. Adv Exp Med Biol. 1996;409:141–146. doi: 10.1007/978-1-4615-5855-2_19
  10. Gangl K, Niederberger V, Valenta R. Multiple grass mixes as opposed to single grasses for allergen immunotherapy in allergic rhinitis. Clin Exp Allergy. 2013;43(11):1202–1216. doi: 10.1111/cea.12128.
  11. Yman L. Pharmacia: Allergenic Plants. Systematics of common and rare allergens. Version 1.0. CD-ROM. Uppsala, Sweden: Pharmacia Diagnostics, 2000.
  12. Хаитов РМ. Клиническая аллергология: руководство для практических врачей. М.: МЕДПресс-информ. 2002. 624 с.
  13. Павлова КС, Курбачева ОМ. Клинико-экономический анализ терапии больных аллергическим ринитом и атопической бронхиальной астмой с наличием бытовой сенсибилизации. Российский аллергологический журнал. 2006;3:22–27.
  14. Navarro AR. Rinitis. En: SEAIC & Schering-Plough editores. Factores epidemiológicos, clínicos y socioeconómicos de las enfermedades alérgicas en España en. 2005:107–131.
  15. Canova C, Heinrich J, Anto JM, Leynaert B, Smith M, Kuenzli N, et al. The influence of sensitisation to pollens and moulds on seasonal variations in asthma attacks. Eur Respir J. 2013;42(4):935–945. doi: 10.1183/09031936.00097412.
  16. Grobe K, Becker WM, Schlaak M, Petersen A. Grass group I allergens (beta-expansins) are novel, papain-related proteinases. Eur J Biochem. 1999;263(1):33–40. doi: 10.1046/j.1432-1327.1999.00462.x.
  17. Focke M, Mahler V, Ball T, Sperr WR, Majlesi Y, Valent P, et al. Nonanaphylactic synthetic peptides derived from B cell epitopes of the major grass pollen allergen, Phl p 1, for allergy vaccination. FASEB J. 2001;15(11):2042–2044. doi: 10.1096/fj.01-0016fje.
  18. Sliwa-Tomczok W, Tomczok J, Dosch IM, Becker WM, Behrendt H. Immunomicroscopic identification of major allergens in pollen of cat’s tail grass (Phleum pratense L.). Pneumologie. 2001;55(5):224–225. doi: 10.1055/s-2001-13944.

Views

Abstract - 44

PDF (Russian) - 10

Cited-By


PlumX

Dimensions



Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies