Changes of the Immunophenotypic Spectrum and the Enzymatic Profile of Peripheral Blood Lymphocytes in Infants with Hypertrophy of the Pharyngeal Tonsil

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Abstract

Objective: to study immunophenotype and NAD- and NAD(P)-dependent dehydrogenase of blood lymphocytes activity indicators in children with hypertrophy of the pharyngeal tonsils (HPT).

Methods: 57 children aged 1–3 years with HPT were examined. The focus group included 35 healthy children of the similar age. The number of СD3+-, СD4+-, СD8+-, СD16+/56+-, СD19+-cells in the blood was determined by flow cytometry. The activity of NAD(P)-dependent dehydrogenase was studied by the method of A. Savchenko and coauth. (1989).

Results: The changes of immunophenotypic spectrum of peripheral blood lymphocytes in infants with HPT have been revealed. The increase of ribose-5-phosphate and NADN-dependent reactions of macromolecular synthesis, the reduction of malataspartat shunt role in cell energy, the reduction of anaerobic lactate dehydrogenase reaction, the compensatory increase in the glycerol-3-phosphate dehydrogenase activity, the high substrate flow of the citric acid cycle and the reduced level of glutathione have been fixed. The correlation analysis has showed increase in the number of correlations between indicators of investigated oxidoreductase activity in blood lymphocytes in children with HPT and the high level of correlation between the metabolic reactions of the mitochondrial compartment.

Conclusion: the change of immunophenotype, enzymatic activity, correlation pattern of connection between intracellular enzymes of peripheral blood lymphocytes have been revealed in children aged 1–3 years with HPT.

About the authors

Lyudmila Mikhaylovna Kurtasova

Krasnoyarsk State Medical University; Krasnoyarsk Regional Center for Prevention and Control of AIDS

Author for correspondence.
Email: sibmed-obozrenie@yandex.ru
MD, PhD, Professor Россия

Natal'ya Aleksandrovna Shakina

Krasnoyarsk Regional Center for Prevention and Control of AIDS

Email: imunolog@aids.krsn.ru
MD, PhD Россия

Tat'yana Viktorovna Lubnina

Krasnoyarsk Regional Center for Prevention and Control of AIDS

Email: Lubnina@aids.krsn.ru
MD, PhD Россия

Anna Igorevna Nikolaeva

Krasnoyarsk State Medical University

Email: annanikolaevalor@mail.ru
MD, PhD Россия

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