Transamination in syncytiotrophoblast of placenta villi in parturient women who suffered the acute form of cmvi at the third trimester of gestation

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Abstract

Aim: to study the process of proteins transamination in syncytiotrophoblast of placenta villi of women who suffered the acute form of cytomegalovirus (CMV) infection during pregnancy.

Methods: 30 pregnant women with CMV infection recurrence at the 25–28th week of pregnancy were examined. The activity of γ-glutamyltransferase in the peripheral blood of pregnant women was determined by spectrophotometry at the device «Stat-Fax-2100» (The USA). Hsp-70 and caspase-3 in placenta homogenate were found out with serological methods. The activity of glutamatdehydrogenase and pyridoxal-5-phosphatase was studied with histochemical method of Z. Loyd at the placenta slice of parturient women. The apoptotic changes in syncytiotrophoblast nuclei were defined by ISEL-method.

Results. The peripheral blood of CMV-seropositive parturient women showed a reduction of γ-glutamyltransferase in 1.30 times. Histochemically we identified the reduction of reaction products' concentration in response to pyridoxal-5-phosphate by 2.14 times, to glutamatdehydrogenase by 1,57 times. At the same time there was an increase of caspase-3 in 2,8 times and reduction of Hsp70 in 2.6 times in placenta homogenate. The number of apoptotic changes in syncytiotrophoblast nuclei increased by 4 times.

Conclusion. Worsening of CMV infection in the period 25–28 weeks of pregnancy leads to disruption of amino acid metabolism in the placenta, causing structural and functional and metabolic adjustment, and is one of the reasons for slow growth and lack of development of the fetus. 

About the authors

Mikhail Timofeevich Lutsenko

Far Eastern Scientific Center of Physiology and Pathology of Respiration

Author for correspondence.
Email: lucenkomt@mail.ru
MD, PhD, professor, head of the laboratory Россия

Irina Anatol'evna Andrievskaya

Far Eastern Scientific Center of Physiology and Pathology of Respiration

Email: irina-andrievskaja@rambler.ru
PhD, Cheif researchet at the laboratory Россия

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