ENDOGENOUS INTOXICATION AND BIOCHEMICAL PROTECTION IN CHILDREN WITH CELIAC DISEASE: STATE ASSESMENT AND CORRELATION-REGRESSION ANALYSIS

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Abstract


Aim: The purpose of the study was to determine the characteristics of endogenous intoxication parameters, biochemical protection and reveal their
interaction in children with celiac disease. Materials and methods: 81 children aged from 1 to 16 years with celiac disease were examined in acute and remission periods. In erythrocytes, blood serum and urine we determined low and moderate molecular weight substances (LMMWS), oligopeptides (OP); in erythrocytes — the value of erythrocyte mechanical hemolysis (MH), malondialdehyde (MDA) content, the activity of glutathione reductase (GR) and superoxide dismutase (SOD); in blood serum — ceruloplasmin (CP) level, alcohol dehydrogenase (ADH) activity; in erythrocytes and blood serum — glutathione transferase (GT), and calculated intoxication index (II). Results: In children with celiac disease in acute and remission periods LMMWS, OP, II levels in blood were statistically significantly high, while LMMWS level in urine was low. In both periods MH activity was high (p <0.001), and GSR (p <0.001) and SOD (p <0.01) levels were low. We revealed the correlation between MDA and II (r =0.67; p =0.006), erythrocyte LMMWS and SOD (r = -0.61; p =0.015), erythrocyte LMMWS and ADH (r =0.62; p =0.006), between GT and OP in urine (r = -0.31; p =0.026), GT and MDA (r =0.68; p =0.000), GT and MH (r = -0.46; p =0.004), between MDA and CP (r =0.57; p =0.002) that made it possible to develop the models of dependence of the parameters in relation to each other. Conclusion: In celiac disease there is endogenous intoxication. The changes of the first and the second phases of biotransformation, antioxidant protection is an essential factor of the disease pathogenesis, since they have an effect on endogenous intoxication formation that should be taken into consideration in therapy.


I. D. Uspenskaya

Nizhny Novgorod Research Institute of Children Gastroenterology

Author for correspondence.
Email: iusp@mail.ru

Russian Federation

PhD, Head of the Department of the Clinical picture of the small intestine pathology of Nizhny Novgorod Research Institute of Children Gastroenterology

Conflict of interests
The authors have indicated they have no financial relationships relevant to this article to disclose.

E. I. Shabunina

Nizhny Novgorod Research Institute of Children Gastroenterology, Russian Federation

Email: niidg@mail.ru

Russian Federation доктор медицинских наук, профессор, директор ННИИДГ Адрес: 603950, Нижний Новгород, ул. Семашко, д. 22, тел.: +7 (831) 436-63-70

E. A. Zhukova

Nizhny Novgorod Research Institute of Children Gastroenterology, Russian Federation

Email: zhulenn@mail.ru

Russian Federation доктор медицинских наук, профессор, заместитель директора по научной работе ННИИДГ Адрес: 603950, Нижний Новгород, ул. Семашко, д. 22, тел.: +7 (831) 436-62-46

M. V. Erzutova

Nizhny Novgorod Research Institute of Children Gastroenterology, Russian Federation

Email: ermariva@mail.ru

Russian Federation кандидат медицинских наук, научный сотрудник отдела Клиники патологии тонкой кишки ННИИДГ Адрес: 603950, Нижний Новгород, ул. Семашко, д. 22, тел.: +7 (831) 436-01-13

L. V. Korkotashvili

Nizhny Novgorod Research Institute of Children Gastroenterology, Russian Federation

Email: lvkor@inbox.ru

Russian Federation кандидат биологических наук, заведующая лабораторно-диагностическим отделом ННИИДГ Адрес: 603950, Нижний Новгород, ул. Семашко, д. 22, тел.: +7 (831) 436-54-60

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