PARATHYREOIDECTOMY IN RATS USING MICROSURGERY AND MEDICAL ADHESIVE SULFACRYLATE

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Abstract

Background: One of the main difficulties in assessing the effectiveness of etiologic treatment of hypoparathyroidism is absence of its standardized model. Objective: The aim of the study was to develop a method of hypoparathyroidism modeling. Methods: Controlled nonrandomized study was performed on female Wistar rats aged 10 month. Group 1 (n =14) was performed with destruction of parathyroid glands with electrocoagulation; group 2 (n =12) — parathyroidectomy by the developed method. In 3 and 14 days after the surgery the levels of ionized calcium, parathyroid hormone, number of white blood cells, blood leukocyte formula, indicators of immunological tests, histological examination of organs in the area of operations were performed. Statistical analysis was performed using the nonparametric Mann–Whitney-Wilcoxon test. Results: Selection of animals by sex and age criteria, angular skin incision, use of the operating microscope, microsurgical techniques, extirpation of parathyroid glands via resection of thyroid gland with the closure of the wound defect glue appeared to be the distinctive features of the developed method. In 14 days the group 2 showed decrease in ionized calcium (p =0.016), PTH (p =0.094), leucocytes (p =0.004), PI (р =0.003), spontaneous NBT test (р =0,004), induced NBT test (р =0.003) compared with group 1. Histological examination in the group 2 revealed no changes in thyroid gland, thin connective tissue capsule, cavity with a small amount of glue, however, there were determined foci of necrosis with perifocal inflammation in the group 1. Conclusion: Combination of several techniques allowed to simulate metabolic disorders with persistent hypocalcemia as well as lack of mortality in early postoperative period.

 

About the authors

A. V. Ausheeva

Scientific Center of Reconstructive and Restorative Surgery, Irkutsk, Russian Federation

Author for correspondence.
Email: ava_irk@inbox.ru

аспирант НЦРВХ
Адрес: 664003, Иркутск, ул. Борцов Революции, д. 1, тел.: +7 (3952) 40-76-66

Россия

S. A. Lepekhova

Scientific Center of Reconstructive and Restorative Surgery, Irkutsk, Russian Federation
Irkutsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, Russian Federation
Irkutsk State Medical University, Russian Federation

Email: lepekhova_sa@mail.ru

доктор биологических наук, заведующая научным отделом экспериментальной
хирургии с виварием НЦРВХ, главный научный сотрудник отдела медико-биологических исследований и техно-
логий ИНЦ СО РАН, ассистент кафедры госпитальной хирургии ИГМУ
Адрес: 664003, Иркутск, ул. Борцов Революции, д. 1, тел.: +7 (3952) 40-76-66

Россия

O. A. Gol'dberg

Scientific Center of Reconstructive and Restorative Surgery, Irkutsk, Russian Federation

Email: ava_irk@inbox.ru

кандидат медицинских наук, ведущий научный сотрудник научно-лабораторного отдела
НЦРВХ
Адрес: 664003, Иркутск, ул. Борцов Революции, д. 1, тел.: +7 (3952) 40-76-66

Россия

E. A. Il'icheva

Scientific Center of Reconstructive and Restorative Surgery, Irkutsk, Russian Federation

Email: lena_isi@mail.ru

доктор медицинских наук, профессор, заведующая научным отделом клинической
хирургии НЦРВХ
Адрес: 664003, Иркутск, ул. Борцов Революции, д. 1, тел.: +7 (3952) 40-78-28

Россия

T. M. Andaeva

Irkutsk Regional Clinical Hospital, Russian Federation

Email: andaeva_tm@iokb.ru

заведующая центром нуклеарной диагностики ИОКБ
Адрес: 664049, Иркутск, мкрн Юбилейный, д. 100, тел.: +7 (3952) 40-76-66

Россия

L. B. Koryakina

Scientific Center of Reconstructive and Restorative Surgery, Irkutsk, Russian Federation
Irkutsk Regional Clinical Hospital, Russian Federation

Email: koriakina-asld@rambler.ru

кандидат медицинских наук, старший научный сотрудник научно-лабораторного
отдела НЦРВХ, заведующая лабораторным отделом ИОКБ
Адрес: 664003, Иркутск, ул. Борцов Революции, д. 1, тел.: +7 (3952) 40-76-66

Россия

I. S. Kurganskii

Irkutsk State Medical University, Russian Federation

Email: kurg.is@mail.ru

младший научный сотрудник научного отдела экспериментальной хирургии с виварием
НЦРВХ
Адрес: 664003, Иркутск, ул. Борцов Революции, д. 1, тел.: +7 (3952) 40-76-66

Россия

L. V. Zaritskaya

Scientific Center of Reconstructive and Restorative Surgery, Irkutsk, Russian Federation

Email: zaritskaya_lv@igmapo.ru

кандидат биологических наук, научный сотрудник научного отдела эксперимен-
тальной хирургии с виварием НЦРВХ
Адрес: 664003, Иркутск, ул. Борцов Революции, д. 1, тел.: +7 (3952) 40-76-66

Россия

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