THE USE OF HYDROSOL HEXACIANFERRATE (II) FERRUM (III) FOR DEVELOPING DIAGNOSTIC LATERAL FLOW TESTS

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Abstract

On the basis of synthesized negatively charged hydrosol hexacianferrate (II) ferrum (III) (HCFF) by diameter 10–20 нм are received stable conjugates with antibodies and antigens glycoprotein and lipopolysaccharide (LPS) nature. Synthesized hydrosol (HCFF) is new type of a disperse phase in the lateral flow assay. Conjugates mentioned above were applied for construction lateral flow tests-systems for revealing cholera toxin, the rabbit antibodies to recombinant glycoproteine complex from Micobaсterium tuberculosis H37 Rv, human immunoglobulin, LPS antigens S. typhimurium, S. enteritidis. Developed lateral flow tests-systems had high analysis speed (5–7 min), good specificity and sensitivity: on cholera toxin of 2,0 ug / ml, on LPS antigens S. typhimurium, S. enteritidis 0,5 ug / ml.

 

About the authors

S. N. Skopinskaya

Federal state unitary enterprise «State scientific research institute of biological engineering» Federal medical-biology agency, Moscow

Author for correspondence.
Email: niibp@dol.ru
кандидат биологических наук, ведущий научный сотрудник ФГУП Государственного научно-исследовательского института биологического приборостроения Федерального медико- биологического агентства России Адрес: 125424, Москва, Волоколамское шоссе, д. 75, к. 1 Тел./факс: (495) 491- 73-72 Russian Federation

S. P. Yarkov

Federal state unitary enterprise «State scientific research institute of biological engineering» Federal medical-biology agency, Moscow

Email: niibp@dol.ru
доктор биологических наук, начальник отдела спектральных методов анализа ФГУП Государственного научно-исследовательского института биологического приборостроения Федерального медико- биологического агентства России Адрес: 125424, Москва, Волоколамское шоссе, д. 75, к. 1 Тел./факс: (495) 491- 73-72 Russian Federation

V. N. Zlobin

Federal state unitary enterprise «State scientific research institute of biological engineering» Federal medical-biology agency, Moscow

Email: niibp@dol.ru
доктор технических наук, профессор, директор ФГУП Государственного научно- исследовательского института биологического приборостроения Федерального медико-биологического агентства России Адрес: 125424, Москва, Волоколамское шоссе, д. 75, к. 1 Тел./факс: (495) 491- 73-72 Russian Federation

Kh. Kh. Valiev

Institute of applied mechanics Russian academy of science, Moscow

Email: hhvlv@mail.ru
кандидат физико-математических наук, старший научный сотрудник Отдела механики структурированных и гетерогенных сред ФГБУ науки Института прикладной механики Российской академии наук Адрес: 125040, Москва, Ленинградский проспект, д. 7 Тел.: (495) 946-17-64 Russian Federation

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