IMMUNOCYTOCHEMICAL CHARACTERIZATION H2S-POSITIVE NEURONS IN THE NUCLEI OF BULBAR CARDIOVASCULAR CENTER IN THE DEVELOPMENT OF RENOVASCULAR HYPERTENSION

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Abstract


Immunocytochemical method in Wistar rats in 6 nuclei of the medulla oblongata, related to the cardiovascular center, identified nerve cells (H2S-neurons) with a positive reaction to cystathionine β-synthase (CBS) — an enzyme involved in the synthesis of hydrogen sulfide in the brain. Found that in rats with renovascular hypertension only after 6 weeks of hypertension in the neurons of the reticular nuclei observed a significant reduction of CBS, while the number of H2S-positive cells in this period does not change significantly. Only at week 12 renovascular hypertension notes marked reduction in the proportion of positive H2S-neurons in the reticular nuclei of most of the medial zone, although further reduction in the intensity of the enzymatic reaction in the neurons were observed. Between 14-16 weeks of hypertension to further reduce the proportion of neurons and H2S-content of the enzyme is not observed. In a single nucleus and reticular lateral nucleus, which receive afferent impulses in the development of renovascular hypertension specify the minimum change of the indicators.

 


V. M. Chertok

Vladivostok State Medical University

Author for correspondence.
Email: chertokv@mail.ru

Russian Federation доктор медицинских наук, профессор, заведующий кафедрой анатомии человека ГБОУ ВПО «Владивостокский государственный медицинский университет» Минздравсоцразвития России Коцюба Александр Евгеньевич, кандидат медицинских наук, доцент кафедры анатомии человека ГБОУ ВПО «Владивостокский государственный медицинский университет» Минздравсоцразвития России Адрес: 690990, Владивосток, пр-т Острякова, д. 2 Тел.: (4232) 45-34-73

A. E. Kotsyuba

Vladivostok State Medical University

Email: chertokv@mail.ru

Russian Federation

  1. Szabo C. Hydrogen sulphide and its therapeutic potential. Nature Reviews. 2007; 6: 917–935.
  2. Dawe G.S., Han S.P., Bian J.S. et al. Hydrogen sulphide in the hypothalamus causes an ATP-sensitive K+ channel-dependent decrease in blood pressure in freely moving rats. Neuroscience. 2008; 152: 169–177.
  3. Harin S.N., Krandycheva V.V., Shmakov D.N. Metodika suzheniya pochechnoj arterii dlya modelirovaniya renovaskulyarnoj gipertenzii u krys. Byulleten’ `eksperimental’noj biologii i mediciny. 2004; 137 (5): 489–493.
  4. Gadalla M.M., Snayder S.H. Hydrogen sulfide as a gasotransmitter. J. Neurochem. 2010; 113: 14–26.
  5. Roth S.H., Skrajny B., Reiffenstein, R.J. Alteration of the morphology and neurochemistry of the developing mammalian nervous system by hydrogen sulfide. Clin. Exp. Pharmacol. Physiol. 1995; 22: 379–380.
  6. Chertok V.M., Kocyuba A.E., Babich E.V. Nitroksidergicheskie nejrony v nekotoryh yadrah prodolgovatogo mozga cheloveka i krysy. Citologiya. 2009; 51 (7): 612–616.
  7. Guyenet P.G. Role of the ventral medulla oblongata in blood pressure regulation. In: Central Regulation of Autonomic Functions / A.D. Loewy and K.M. Spayer (eds). Oxford University Press. N.Y. 1990. 145–167 p.
  8. Kerchner G.A., Nicoll R.A. Silent synapses and the emergence of a postsynaptic mechanism for LTP. Nat. Rev. Neurosci. 2008; 9: 813–825.
  9. Babich E.V., Chertok V.M, Kocyuba A.E. Nitroksidergicheskie nejrony v yadrah prodolgovatogo mozga u normo- i gipertenzivnyh krys. Byulleten’ `eksperimental’noj biologii i mediciny. 2009; 147 (8):157–160.
  10. Kocyuba A.E., Chertok V.M., Babich E.V. Nitroksidergicheskie nejrony bul’barnogo vazomotornogo centra cheloveka pri arterial’noj gipertenzii. Zhurnal nevrologii i psihiatrii. 2010; 2: 61–65.

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