EVALUATION OF ASSOCIATION BETWEEN 9 GENETIC POLYMORPHISM AND MYOCARDIAL INFARCTION IN THE SIBERIAN POPULATION

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Abstract

Aim: to evaluate association between genetic polymorphism (SNPs) and myocardial infarction (identified in recent GWAS) as markers of high risk of myocardial infarction (MI) in Siberian population. Patients were divided into 2 groups — MI patients and control group (ratio 1:2) and presented the sapmle of population of Novosibirsk (9400 patients, 45–69 years) within international project HAPIEE (Health, Alcohol and Psychosocial factors In Eastern Europe). 200 patients with MI (129 men, 71 women) were included. Control group — individuals without MI (420) matched for age and sex. Genomic DNA was extracted from venous blood by phenol-chloroform extraction. Gene polymorphism of genes tested by real-time PCR according to protocol (probes TaqMan, Applied Biosystems, USA) with the use of ABI 7900HT. The following SNPs were studied: rs28711149, rs499818, rs619203, rs10757278 and rs1333049 (hr. 9), rs1376251, rs2549513, rs4804611, rs17465637. The association of SNP and MI was confirmed for 4 of 9 studied SNPs: rs1333049 (hr. 9), rs10757278 (hr. 9), rs499818 (hr. 6), rs619203 gene ROS1. Heart rate was associated with rs1333049 and rs10757278. Glucose level was associated with rs619203, rs28711149 and rs1376251. Total cholesterol and atherogenic index was associated with rs28711149. For the first time in Russian population the associations of GWAS with myocardial infarction SNPs was detected for rs619203, rs499818, rs1333049 and rs10757278. These genetic markers can be used for assessing the risk of myocardial infarction in Russian population.

 

About the authors

V. N. Maximov

Institute of Internal medicine SB RAMS, Novosibirsk
Novosibirsk State Medical University, department of medical genetics

Author for correspondence.
Email: medik11@mail.ru
доктор медицинских наук, внештатный научный сотрудник лаборатории молекулярно-генетических исследований терапевтических заболеваний НИИ терапии Сибирского отделения РАМН Адрес: 630089, Новосибирск, ул. Б. Богаткова, д. 175/1 Тел./факс: (383) 264-25-16 Россия

I. V. Kulikov

Institute of Internal medicine SB RAMS, Novosibirsk
Institute of Cytology and Genetics SD RAS, Novosibirsk

Email: 248945@mail.ru
кандидат медицинских наук, старший научный сотрудник лаборатории молекулярно- генетических исследований терапевтических заболеваний НИИ терапии Сибирского отделения РАМН Адрес: 630089, Новосибирск, ул. Б. Богаткова, д. 175/1 Тел./факс: (383) 264-25-16 Россия

P. S. Orlov

Institute of Cytology and Genetics SD RAS, Novosibirsk

Email: orlovpavel86@gmail.com
аспирант Института цитологии и генетики Сибирского отделения РАН Адрес: 630090, Новосибирск, пр-т Лаврентьева, д. 10 Россия

V. V. Gafarov

Institute of Internal medicine SB RAMS, Novosibirsk

Email: valery.gafarov@gmail.com
доктор медицинских наук, профессор, заведующий лабораторией психологических и социологических проблем терапевтических заболеваний НИИ терапии Сибирского отделения РАМН Адрес: 630089, Новосибирск, ул. Б. Богаткова, д. 175/1 Тел./факс: (383) 264-25-16 Россия

S. K. Malyutina

Institute of Internal medicine SB RAMS, Novosibirsk
Novosibirsk State Medical University, department of medical genetics

Email: smalyutina@hotmail.com
доктор медицинских наук, профессор, руководитель группы неинвазивной диа- гностики лаборатории этиопатогенеза и клиники внутренних заболеваний НИИ терапии Сибирского отделения РАМН Адрес: 630089, Новосибирск, ул. Б. Богаткова, д. 175/1 Тел./факс: (383) 264-25-16 Россия

A. G. Romashchenko

Institute of Cytology and Genetics SD RAS, Novosibirsk

Email: romasch@bionet.nsc.ru
кандидат биологических наук, заведующая лабораторией молекулярных основ генетики животных Института цитологии и генетики Сибирского отделения РАН Адрес: 630090, Новосибирск, пр-т Лаврентьева, д. 10 Тел: (383) 363-49-74 Россия

M. I. Voevoda

Institute of Internal medicine SB RAMS, Novosibirsk
Institute of Cytology and Genetics SD RAS, Novosibirsk

Email: mvoevoda@ya.ru
доктор медицинских наук, профессор, член-корреспондент РАМН, директор НИИ терапии Сибирского отделения РАМН Адрес: 630089, Новосибирск, ул. Б. Богаткова, д. 175/1 Тел./факс: (383) 264-25-16 Россия

References

  1. Horne B.D., Carlquist J.F., Muhlestein J.B. et al. Associations with myocardial infarction of six polymorphisms selected from a three-stage genome-wide association study. Am. Heart J. 2007; 154 (5): 969–975.
  2. Larson M.G., Atwood L.D., Benjamin E.J. et al. Framingham Heart Study 100K project: genome-wide associations for cardiovascular disease outcomes. BMC Med. Genet. 2007; 8 (1): 5.
  3. Ozaki K., Tanaka T. Genome-wide association study to identify single-nucleotide polymorphisms conferring risk of myocardial infarction. Methods Mol. Med. 2006; 128: 173–180.
  4. Samani N.J., Erdmann J., Hall A.S. et al. Genomewide association analysis of coronary artery disease. N. Engl. J. Med. 2007; 2357: 443–453.
  5. Van der Net J.B., Oosterveer D.M., Versmissen J. et al. Replication study of 10 genetic polymorphisms associated with coronary heart disease in a specific high-risk population with familial hypercholesterolemia. Eur. Heart J. 2008; 29 (18): 2195–2201.
  6. Hiura Y., Fukushima Y., Yuno M. et al. Validation of the association of genetic variants on chromosome 9p21 and 1q41 with myocardial infarction in a Japanese population. Circ. J. 2008; 72 (8): 1213–1217.
  7. Schunkert H., Götz A., Braund P. et al. Repeated replication and a prospective meta-analysis of the association between chromosome 9p21.3 and coronary artery disease. Circulation. 2008; 117 (13): 1675–1684.
  8. Shen G.Q., Li L., Rao S. et al. Four SNPs on chromosome 9p21 in a South Korean population implicate a genetic locus that confers high cross-race risk for development of coronary artery disease. Arterioscler. Thromb. Vasc. Biol. 2008; 28 (2): 360–365.
  9. MONICA Monograph and Multimedia Sourcebook. World,s largest study of heart disease, stroke, risk factors, and population trends 1979–2002. Ed. by Hugh Tunstall-Pedoe (with 64 other contributors for the WHO MONICA Project). WHO, Geneva. 2003. 237.
  10. Smith K., Kalcko S., Kantor Ch. . Pul's-elektroforez i metody raboty s bol'shimi molekulami DNK. Analiz genoma. Pod red. K. Deivisa (per. s angl.) [Pulse Electrophoresis and Methods of Work with Large DNA Molecules. Analysis of the Genome. Edited by K. Davis. Translated from English]. Moscow, Mir, 1990. p. 58–94.
  11. Helgadottir A., Thorleifsson G., Magnusson K.P. et al. The same sequence variant on 9p21 associates with myocardial infarction, abdominal aortic aneurysm and intracranial aneurysm. Nat. Genet. 2008, 40 (2): 217–224.
  12. Anderson C.D., Biffi A., Rost N.S. et al. Chromosome 9p21 in Ischemic Stroke. Population Structure and Meta-Analysis. Stroke. 2010, 41 (6):1123–1131
  13. Ellis K.L., Pilbrow A.P., Frampton C.M. et al. A Common Variant at Chromosome 9P21.3 Is Associated with Age of Onset of Coronary Disease but Not Subsequent Mortality. Circ. Cardiovasc. Genet. 2010; 3 (3): 286–293.
  14. Buysschaert I., Carruthers K.F., Dunbar D.R. et al. A variant at chromosome 9p21 is associated with recurrent myocardial infarction and cardiac death after acute coronary syndrome: The GRACE Genetics Study. Eur. Heart J. 2010: 31 (9): 1132–1141
  15. Shiffman D., Ellis S.G., Rowland C.M. et al. Identification of four gene variants associated with myocardial infarction. Am. J. Hum. Genet. 2005; 77: 596–605.
  16. Koch W., Hoppmann P., Schömig A., Kastrati A. Variations of specific non-candidate genes and risk of myocardial infarction: A replication study. Int. J. Cardiol. 2011; 147 (1): 38–41.
  17. Theodoraki E.V., Nikopensius T., Suhorutsenko J. et al. ROS1 Asp2213Asn polymorphism is not associated with coronary artery disease in a Greek case-control study. Clin. Chem. Lab. Med. 2009; 47 (12): 1471–1473.
  18. Yamada Y., Izawa H., Ichihara S. et al. Prediction of the risk of myocardial infarction from polymorphisms in candidate genes. N. Engl. J. Med. 2002; 347: 1916–1923.
  19. Myocardial Infarction Genetics Consortium Genome-wide association of early-onset myocardial infarction with single nucleotide polymorphisms and copy number variants. Nat. Genet. 2009; 41 (3): 334–341.
  20. Bressler J., Folsom A.R., Couper D.J. et al. Genetic variants identified in a European genome-wide association study that were found to predict incident coronary heart disease in the atherosclerosis risk in communities study. Am. J. Epidemiol. 2010; 171 (1): 14–23.

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