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CORRECTION OF CRONIC LIVER FAILURE BY TRANSPLANTATION OF LIVER CELLS SUSPENSION AND CELL-ENGINEERING DESIGNS (EXPERIMENTAL INVESTIGATION)
- Authors: Got'e S.V.1, Shagidulin M.Y.1, Onishchenko N.A.1, Krashennikov M.E.2, Il'inskii I.M.1, Mozheiko N.P.2, Lyundup A.V.3, Volkova E.A.2, Petrakov K.I.2, Avramov P.V.2, Perova N.V.2, Sevast'yanov V.I.2
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Affiliations:
- Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation I.M. Sechenov Moscow State Medical University, Moscow, Russian Federation
- Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation
- I.M. Sechenov Moscow State Medical University, Moscow, Russian Federation
- Issue: Vol 68, No 4 (2013)
- Pages: 44-51
- Section: TRANSPLANTOLOGY: CURRENT ISSUES
- Published:
- URL: https://vestnikramn.spr-journal.ru/jour/article/view/195
- DOI: https://doi.org/10.15690/vramn.v68i4.610
- ID: 195
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Abstract
On an experimental model of chronic fibrotic liver damage (male rats Wistar (n=60), damage of CCl4, the duration of the experiment 90 days) it was studied the effectiveness of cell therapy for the correction of chronic liver failure. These rats were divided into 3 experimental groups: in the Ist group (control, n=10) isotonic saline (650 mkl.) was injected; in the IInd group (n=20) suspension of liver cells was applicated in a dose 8-10×106 cells; in the IIIrd group (n=30) suspension of liver cells and bone marrow cells (mesenchymal stromal cells) in ratio 5:1 were used as cell associates on microparticles injectable heterogeneous biopolymer hydrogel «SpheroGEL» (cell-engineering design) in common dose 8-10×106 cells . It was ascertained that in the 2nd and in the 3rd groups the accelerated normalization of disturbed liver functional indices (ALT, AST, ALP) took place – to 30 days, but in the control group only to 90 days. The reliable differences in rats of normalization of functional indices were absent between the IInd and the IIIrd groups. But in 90 days by using special histological dyeing it was found out that defibrotic processes in liver tissue were more expressed in the IIIrd group in comparison with the IIInd group. Received results were consequence of prolonged vital activity of cells (liver cells and mesenchymal stromal bone marrow cells) into cell-engineering designs, which were transplanted in the IIIrd group. The obtained effect can be explained by that the developed cell-engineering designs provide adequate conditions for prolonged vital activity of the transplanted cells.
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About the authors
S. V. Got'e
Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian FederationI.M. Sechenov Moscow State Medical University, Moscow, Russian Federation
Author for correspondence.
Email: viksev@yandex.ru
RAMS academician, PhD, Professor, Director of Federal State Budgetary Institution of Transplantology and Artificial Organs named after acad. V.I. Shumakov, Head of the Department of Transplantology and Artificial Organs Federal State Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 196-18-03 Россия
M. Yu. Shagidulin
Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian FederationI.M. Sechenov Moscow State Medical University, Moscow, Russian Federation
Email: dr.shagidulin@mail.ru
PhD, Head of the Department of Experimental Transplantology and Artificial Organs, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov, Associate Professor of Transplantology and Artificial Organs Federal State Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 196-87-90 Россия
N. A. Onishchenko
Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian FederationI.M. Sechenov Moscow State Medical University, Moscow, Russian Federation
Email: viksev@yandex.ru
PhD, Professor, Head of Stem Cells Laboratory, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov, Department of Transplantology and Artificial Organs Federal State Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-45-31 Россия
M. E. Krashennikov
Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation
Email: krashen@rambler.ru
PhD, Senior Research Worker, Laboratory of Stem Cells, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-45-31 Россия
I. M. Il'inskii
Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian FederationI.M. Sechenov Moscow State Medical University, Moscow, Russian Federation
Email: viksev@yandex.ru
PhD, Professor, Head of the Department of Clinical Pathology, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov, Department of Transplantology and Artificial Organs Federal State Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-64-28 Россия
N. P. Mozheiko
Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation
Email: npm_72@mail.ru
PhD, Physician, Pathology Department, Federal state budgetary institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-64-28 Россия
A. V. Lyundup
I.M. Sechenov Moscow State Medical University, Moscow, Russian Federation
Email: gma.tulkas@gmail.com
PhD, Head of the Department of Biomedical Research, Research Institute of Molecular Medicine, Federal State Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University. Address: 117997, Moscow, Trubetskaya St. 8; tel.: (495) 609-14-00 ext 3051 Россия
E. A. Volkova
Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation
Email: volckowa.g2011@yandex.ru
Head of the Laboratory of Preparation and Carrying out of Experimental Studies, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov. Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-77-05 Россия
K. I. Petrakov
Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation
Email: viksev@yandex.ru
Junior Research Worker, Cardiac Surgery Department №3, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov. Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-64-28 Россия
P. V. Avramov
Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation
Email: viksev@yandex.ru
Head of the Experimental Laboratory with Vivarium, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov. Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-64-28 Россия
N. V. Perova
Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation
Email: viksev@yandex.ru
Россия
V. I. Sevast'yanov
Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation
Email: viksev@yandex.ru
Россия
References
- Chikoteev S.P., Plekhanov A.N. Ocherki khirurgii pecheni i podzheludochnoi zhelezy [Essays on Surgery of Liver and Pancreas]. Irkutsk, 2002. 259 p.
- Got'e S.V., Moisyuk Ya.G. Trends in the development of organ donation and transplantation in the Russian Federation in 2006-2008. Message I (according to the register of the Russian society transplantolog). Vestn. transplantol. i iskusstv. organov = Bulletin of transplantation and artificial organs. 2009; XI (3): 8–16.
- Onishchenko N.A., Lyundup A.V., Shagidulin M.Yu., Krasheninnikov M.E. Sinusoidal liver cells and bone marrow cells as components of a single functional system of regulation restorative morphogenesis in a healthy and damaged liver. Kletochn. transplantol. i tkanev. inzheneriya = Cell transplantology and tissue engineering. 2011; 2 (VI): 73–87.
- Sevast'yanov V.I., Perova N.V., Nemets E.A., Surguchenko V.A., Ponomareva A.S. Primery eksperimental'no-klinicheskogo primeneniya biosovmestimykh materialov v regenerativnoi meditsine. V kn.: Biosovmestimye materialy (uch. pos.). Chast' II. Glava 3. Pod red. V.I. Sevast'yanova i M.P. Kirpichnikova [Examples of Experimental and Clinical Use of Biocompatible Materials for Regenerative Medicine. In: Biocompatible Materials (Manual). Part II. Chapter 3. Edited by V.I. Sevast'yanov and M.P. Kirpichnikov]. Moscow, MIA, 2011. p. 237–252.
- Uyama S., Kaufman P., Kneser U., Vacanti J., Rodriges X. Hepatocyte transplantation using biodegradable matrices in ascorbic acid-deficient rats: comparsion with heterotropically transplanted liver grafts. Transplantation. 2001; 7: 1–7.
- Shumakov V.I., Krasheninnikov M.E., Onishchenko N.A. Kul'tura kletok, soderzhashchaya kletki-predshestvenniki osteogeneza, implantant na ee osnove i ego ispol'zovanie dlya vosstanovleniya tselostnosti kosti. Patent № 2240135. Zaregistrirovan ot 20 noyabrya 2004 g. [Cell Culture, Comprising Osteogenic Precursor Cells, the Implant Based on it and its Use for Restoring the Integrity of the Bone. Patent № 2240135. Registered on November 20, 2004].
- Fiegel H.C., Kaufmann P.M., Kneser U., Kluth D., Rogiers X. Priming of hepatocytes for cell culture by partial hepatectomy prior to cell isolation. Tissue Eng. 2000; 6 (6): 619–626.
- Shagidulin M.Yu., Onishchenko N.A., Krasheninnikov M.E., Il'inskii I.M., Mozheiko N.P., Shmerko N.P., Sevast'yanov V.I., Got'e S.V. Survival liver cells immobilized on scaffolds with 3D-modeling hepatic insufficiency. Vestn. transplantol. i iskusstv. organov = Bulletin of transplantation and artificial organs. 2011; 3 (XIII): 59–66.
- Charles. R., Lu L., Qian S., Fung J. Stromal cell-based immunotherapy in transplantation. Immunotherapy. 2011; 3: 1471–1485.
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