CORRECTION OF CRONIC LIVER FAILURE BY TRANSPLANTATION OF LIVER CELLS SUSPENSION AND CELL-ENGINEERING DESIGNS (EXPERIMENTAL INVESTIGATION)

Cover Page
  • Authors: Got'e S.V.1, Shagidulin M.Y.1, Onishchenko N.A.1, Krashennikov M.E.2, Il'inskii I.M.1, Mozheiko N.P.2, Lyundup A.V.3, Volkova E.A.2, Petrakov K.I.2, Avramov P.V.2, Perova N.V.2, Sevast'yanov V.I.2
  • Affiliations:
    1. Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation I.M. Sechenov Moscow State Medical University, Moscow, Russian Federation
    2. Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation
    3. I.M. Sechenov Moscow State Medical University, Moscow, Russian Federation
  • Issue: Vol 68, No 4 (2013)
  • Pages: 44-51
  • Section: TRANSPLANTOLOGY: CURRENT ISSUES
  • URL: https://vestnikramn.spr-journal.ru/jour/article/view/195
  • DOI: https://doi.org/10.15690/vramn.v68i4.610
  • Cite item

Abstract


On an experimental model of chronic fibrotic liver damage (male rats Wistar (n=60), damage of CCl4, the duration of the experiment 90 days) it was studied the effectiveness of cell therapy for the correction of chronic liver failure. These rats were divided into 3 experimental groups: in the Ist group (control, n=10) isotonic saline (650 mkl.) was injected; in the IInd group (n=20) suspension of liver cells was applicated in a dose 8-10×106 cells; in the IIIrd group (n=30) suspension of liver cells and bone marrow cells (mesenchymal stromal cells) in ratio 5:1 were used as cell associates on microparticles injectable heterogeneous biopolymer hydrogel «SpheroGEL» (cell-engineering design) in common dose 8-10×106 cells . It was ascertained that in the 2nd and in the 3rd groups the accelerated normalization of disturbed liver functional indices (ALT, AST, ALP) took place – to 30 days, but in the control group only to 90 days. The reliable differences in rats of normalization of functional indices were absent between the IInd and the IIIrd groups. But in 90 days by using special histological dyeing it was found out that defibrotic processes in liver tissue were more expressed in the IIIrd group in comparison with the IIInd group. Received results were consequence of prolonged vital activity of cells (liver cells and mesenchymal stromal bone marrow cells) into cell-engineering designs, which were transplanted in the IIIrd group. The obtained effect can be explained by that the developed cell-engineering designs provide adequate conditions for prolonged vital activity of the transplanted cells.

 


S. V. Got'e

Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation
I.M. Sechenov Moscow State Medical University, Moscow, Russian Federation

Author for correspondence.
Email: viksev@yandex.ru

Russian Federation RAMS academician, PhD, Professor, Director of Federal State Budgetary Institution of Transplantology and Artificial Organs named after acad. V.I. Shumakov, Head of the Department of Transplantology and Artificial Organs Federal State Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 196-18-03

M. Yu. Shagidulin

Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation
I.M. Sechenov Moscow State Medical University, Moscow, Russian Federation

Email: dr.shagidulin@mail.ru

Russian Federation PhD, Head of the Department of Experimental Transplantology and Artificial Organs, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov, Associate Professor of Transplantology and Artificial Organs Federal State Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 196-87-90

N. A. Onishchenko

Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation
I.M. Sechenov Moscow State Medical University, Moscow, Russian Federation

Email: viksev@yandex.ru

Russian Federation PhD, Professor, Head of Stem Cells Laboratory, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov, Department of Transplantology and Artificial Organs Federal State Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-45-31

M. E. Krashennikov

Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation

Email: krashen@rambler.ru

Russian Federation PhD, Senior Research Worker, Laboratory of Stem Cells, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-45-31

I. M. Il'inskii

Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation
I.M. Sechenov Moscow State Medical University, Moscow, Russian Federation

Email: viksev@yandex.ru

Russian Federation PhD, Professor, Head of the Department of Clinical Pathology, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov, Department of Transplantology and Artificial Organs Federal State Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-64-28

N. P. Mozheiko

Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation

Email: npm_72@mail.ru

Russian Federation PhD, Physician, Pathology Department, Federal state budgetary institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-64-28

A. V. Lyundup

I.M. Sechenov Moscow State Medical University, Moscow, Russian Federation

Email: gma.tulkas@gmail.com

Russian Federation PhD, Head of the Department of Biomedical Research, Research Institute of Molecular Medicine, Federal State Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University. Address: 117997, Moscow, Trubetskaya St. 8; tel.: (495) 609-14-00 ext 3051

E. A. Volkova

Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation

Email: volckowa.g2011@yandex.ru

Russian Federation Head of the Laboratory of Preparation and Carrying out of Experimental Studies, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov. Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-77-05

K. I. Petrakov

Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation

Email: viksev@yandex.ru

Russian Federation Junior Research Worker, Cardiac Surgery Department №3, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov. Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-64-28

P. V. Avramov

Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation

Email: viksev@yandex.ru

Russian Federation Head of the Experimental Laboratory with Vivarium, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov. Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-64-28

N. V. Perova

Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation

Email: viksev@yandex.ru

Russian Federation

V. I. Sevast'yanov

Federal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation

Email: viksev@yandex.ru

Russian Federation

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