EXPERIMENTAL WHOOPING COUGH OF NONHUMAN PRIMATE

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  • Authors: Kubrava D.T.1, Medkova A.Y.2, Sinyashina L.N.2, Shevtsova Z.V.1, Matua A.Z.1, Kondzharia I.G.1, Barkaya V.S.1, Elistratova Z.V.1, Karataev G.I.1, Mikvabia Z.Y.1, Gintsburg A.L.2
  • Affiliations:
    1. Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia
    2. Gamaleya Scientific Research Institute of Epidemiology and Microbiology, Moscow, Russian Federation
  • Issue: Vol 68, No 8 (2013)
  • Pages: 28-33
  • Section: INFECTIOUS DISEASES: CURRENT ISSUES
  • URL: https://vestnikramn.spr-journal.ru/jour/article/view/156
  • DOI: https://doi.org/10.15690/vramn.v68i8.720
  • Cite item

Abstract


Despite considerable success in study of Bordetella pertussis virulence factors, pathogenesis of whooping cough, duration of B.pertussis bacteria persistence, types and mechanisms of immune response are still keep underinvestigated. It can be explained by the absence of adequate experimental animal model for pertussis study. Our study estimates clinical and laboratory parameters of whooping cough in non-human primates of the Old World in the process of intranasal infection by virulent B.pertussis bacteria. Also the duration of B.pertussis bacteria persistence in animals was investigated. 14 animal units of 4 species of non-human primates of the Old World were used for intranasal infection. The examination of infect animals included: visual exploration of nasopharynx, thermometry, clinical and biochemical blood analyses, identification of B.pertussis, using microbiologic and molecular genetic analyses, estimation of innate and adoptive immune factors.  The development of infectious process was accompanied by generation of B.pertussis bacteria, catarrhal inflammation of nasopharyngeal mucosa, leucocytosis, hypoglycemia specific for pertussis, and activation of innate and adaptive immunity for all primates regardless of specie were seen. While repeated experimental infection in primates single bacterial colonies were registered during only first week after challenge. It occurs like the absence of inflammation of nasopharyngeal mucosa and the lack of laboratory marks of whooping cough, recorded after first challenge. The evident booster effect of humoral immunity was observed. As a model for investigation of B. pertussis bacteria persistence and immune response against whooping cough we suggest the usage of rhesus macaque as more available to experiments.

 


About the authors

D. T. Kubrava

Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia

Author for correspondence.
Email: jenny-vanacha@yandex.ru
postgraduate of Abkhazian Academy of Sciences, Research Institute of Experimental Pathology and Therapy of Abkhazian Academy of Sciences. Address: 384900, Abkhazia, Sukhumi, Trapetsiya Gora St., 27; tel.: (840) 226-77-52

A. Yu. Medkova

Gamaleya Scientific Research Institute of Epidemiology and Microbiology, Moscow, Russian Federation

Email: baburida@yandex.ru

Russian Federation MD, research scientist of the group of Molecular Biology of Pathogenic Microorganisms of N. F. Gamalei Research Institute of Epidemiology and Microbiology. Address: 123098, Moscow, Gamalei St., 18; tel.: (499) 193-61-90

L. N. Sinyashina

Gamaleya Scientific Research Institute of Epidemiology and Microbiology, Moscow, Russian Federation

Email: karataevgi@rambler.ru

Russian Federation MD, senior research scientist of the group of Molecular Biology of Pathogenic Microorganisms of N. F. Gamalei Research Institute of Epidemiology and Microbiology. Address: 123098, Moscow, Gamalei St., 18; tel.: (499) 193-61-90

Z. V. Shevtsova

Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia

Email: niiepit@rambler.ru
PhD, professor, member of RANS, Head of the Laboratory of Immunology and Virology of Research Institute of Experimental Pathology and Therapy of Abkhazian Academy of Sciences. Address: 384900, Abkhazia, Sukhumi, Trapetsiya Gora St., 27; tel.: (840) 226-88-70

A. Z. Matua

Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia

Email: azmatua76@mail.ru
MD, associate professor, senior research scientist of the Laboratory of Immunology and Virology of Research Institute of Experimental Pathology and Therapy of Abkhazian Academy of Sciences. Address: 384900, Abkhazia, Sukhumi, Trapetsiya Gora St., 27; tel.: (840) 226-77-52

I. G. Kondzharia

Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia

Email: niiepit@rambler.ru
MD, associate professor, senior research scientist of the Laboratory of Immunology and Virology of Research Institute of Experimental Pathology and Therapy of Abkhazian Academy of Sciences. Address: 384900, Abkhazia, Sukhumi, Trapetsiya Gora St., 27; tel.: (840) 226-77-52

V. S. Barkaya

Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia

Email: vladbarnii@rambler.ru
MD, professor, deputy director for science, Head of the Laboratory of Experimental Hematology of Research Institute of Experimental Pathology and Therapy of Abkhazian Academy of Sciences. Address: 384900, Abkhazia, Sukhumi, Trapetsiya Gora St., 27; tel.: (840) 226-77-52

Zh. V. Elistratova

Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia

Email: blacksea1942@bk.ru
senior research scientist of the Laboratory of Experimental Hematology of Research Institute of Experimental Pathology and Therapy of Abkhazian Academy of Sciences. Address: 384900, Abkhazia, Sukhumi, Trapetsiya Gora St., 27; tel.: (840) 226-77-52

G. I. Karataev

Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia

Email: karataevgi@rambler.ru
PhD, leading research scientist, team leader of Molecular Biology of Pathogenic Microorganisms of N. F. Gamalei Research Institute of Epidemiology and Microbiology. Address: 123098, Moscow, Gamalei St., 18; tel.: (499) 193-61-90

Z. Ya. Mikvabia

Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia

Email: niiepit@rambler.ru
cor. member of RANS, Director of Research Institute of Experimental Pathology and Therapy of Abkhazian Academy of Sciences. Address: 384900, Abkhazia, Sukhumi, Trapetsiya Gora St., 27; tel.: (840) 226-77-58

A. L. Gintsburg

Gamaleya Scientific Research Institute of Epidemiology and Microbiology, Moscow, Russian Federation

Email: info@gamaleya.org
PhD, professor, member of RAMS, Director of N. F. Gamalei Research Institute of Epidemiology and Microbiology. Address: 123098, Moscow, Gamalei St., 18; tel.: (499) 193-30-01

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