Email this article 
EXPERIMENTAL WHOOPING COUGH OF NONHUMAN PRIMATE
- Authors: Kubrava D.T.1, Medkova A.Y.2, Sinyashina L.N.2, Shevtsova Z.V.1, Matua A.Z.1, Kondzharia I.G.1, Barkaya V.S.1, Elistratova Z.V.1, Karataev G.I.1, Mikvabia Z.Y.1, Gintsburg A.L.2
-
Affiliations:
- Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia
- Gamaleya Scientific Research Institute of Epidemiology and Microbiology, Moscow, Russian Federation
- Issue: Vol 68, No 8 (2013)
- Pages: 28-33
- Section: INFECTIOUS DISEASES: CURRENT ISSUES
- URL: https://vestnikramn.spr-journal.ru/jour/article/view/156
- DOI: https://doi.org/10.15690/vramn.v68i8.720
- ID: 156
Cite item
Full Text
Abstract
Despite considerable success in study of Bordetella pertussis virulence factors, pathogenesis of whooping cough, duration of B.pertussis bacteria persistence, types and mechanisms of immune response are still keep underinvestigated. It can be explained by the absence of adequate experimental animal model for pertussis study. Our study estimates clinical and laboratory parameters of whooping cough in non-human primates of the Old World in the process of intranasal infection by virulent B.pertussis bacteria. Also the duration of B.pertussis bacteria persistence in animals was investigated. 14 animal units of 4 species of non-human primates of the Old World were used for intranasal infection. The examination of infect animals included: visual exploration of nasopharynx, thermometry, clinical and biochemical blood analyses, identification of B.pertussis, using microbiologic and molecular genetic analyses, estimation of innate and adoptive immune factors. The development of infectious process was accompanied by generation of B.pertussis bacteria, catarrhal inflammation of nasopharyngeal mucosa, leucocytosis, hypoglycemia specific for pertussis, and activation of innate and adaptive immunity for all primates regardless of specie were seen. While repeated experimental infection in primates single bacterial colonies were registered during only first week after challenge. It occurs like the absence of inflammation of nasopharyngeal mucosa and the lack of laboratory marks of whooping cough, recorded after first challenge. The evident booster effect of humoral immunity was observed. As a model for investigation of B. pertussis bacteria persistence and immune response against whooping cough we suggest the usage of rhesus macaque as more available to experiments.
About the authors
D. T. Kubrava
Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia
Author for correspondence.
Email: jenny-vanacha@yandex.ru
postgraduate of Abkhazian Academy of Sciences, Research Institute of Experimental Pathology and Therapy of Abkhazian Academy of Sciences. Address: 384900, Abkhazia, Sukhumi, Trapetsiya Gora St., 27; tel.: (840) 226-77-52
A. Yu. Medkova
Gamaleya Scientific Research Institute of Epidemiology and Microbiology, Moscow, Russian Federation
Email: baburida@yandex.ru
MD, research scientist of the group of Molecular Biology of Pathogenic Microorganisms of N. F. Gamalei Research Institute of Epidemiology and Microbiology. Address: 123098, Moscow, Gamalei St., 18; tel.: (499) 193-61-90 Russian Federation
L. N. Sinyashina
Gamaleya Scientific Research Institute of Epidemiology and Microbiology, Moscow, Russian Federation
Email: karataevgi@rambler.ru
MD, senior research scientist of the group of Molecular Biology of Pathogenic Microorganisms of N. F. Gamalei Research Institute of Epidemiology and Microbiology. Address: 123098, Moscow, Gamalei St., 18; tel.: (499) 193-61-90 Russian Federation
Z. V. Shevtsova
Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia
Email: niiepit@rambler.ru
PhD, professor, member of RANS, Head of the Laboratory of Immunology and Virology of Research Institute of Experimental Pathology and Therapy of Abkhazian Academy of Sciences. Address: 384900, Abkhazia, Sukhumi, Trapetsiya Gora St., 27; tel.: (840) 226-88-70
A. Z. Matua
Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia
Email: azmatua76@mail.ru
MD, associate professor, senior research scientist of the Laboratory of Immunology and Virology of Research Institute of Experimental Pathology and Therapy of Abkhazian Academy of Sciences. Address: 384900, Abkhazia, Sukhumi, Trapetsiya Gora St., 27; tel.: (840) 226-77-52
I. G. Kondzharia
Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia
Email: niiepit@rambler.ru
MD, associate professor, senior research scientist of the Laboratory of Immunology and Virology of Research Institute of Experimental Pathology and Therapy of Abkhazian Academy of Sciences. Address: 384900, Abkhazia, Sukhumi, Trapetsiya Gora St., 27; tel.: (840) 226-77-52
V. S. Barkaya
Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia
Email: vladbarnii@rambler.ru
MD, professor, deputy director for science, Head of the Laboratory of Experimental Hematology of Research Institute of Experimental Pathology and Therapy of Abkhazian Academy of Sciences. Address: 384900, Abkhazia, Sukhumi, Trapetsiya Gora St., 27; tel.: (840) 226-77-52
Zh. V. Elistratova
Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia
Email: blacksea1942@bk.ru
senior research scientist of the Laboratory of Experimental Hematology of Research Institute of Experimental Pathology and Therapy of Abkhazian Academy of Sciences. Address: 384900, Abkhazia, Sukhumi, Trapetsiya Gora St., 27; tel.: (840) 226-77-52
G. I. Karataev
Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia
Email: karataevgi@rambler.ru
PhD, leading research scientist, team leader of Molecular Biology of Pathogenic Microorganisms of N. F. Gamalei Research Institute of Epidemiology and Microbiology. Address: 123098, Moscow, Gamalei St., 18; tel.: (499) 193-61-90
Z. Ya. Mikvabia
Research Institute of Experimental Pathology and Therapy, Abkhazian Academy of Sciences, Sukhum, Abkhazia
Email: niiepit@rambler.ru
cor. member of RANS, Director of Research Institute of Experimental Pathology and Therapy of Abkhazian Academy of Sciences. Address: 384900, Abkhazia, Sukhumi, Trapetsiya Gora St., 27; tel.: (840) 226-77-58
A. L. Gintsburg
Gamaleya Scientific Research Institute of Epidemiology and Microbiology, Moscow, Russian Federation
Email: info@gamaleya.org
PhD, professor, member of RAMS, Director of N. F. Gamalei Research Institute of Epidemiology and Microbiology. Address: 123098, Moscow, Gamalei St., 18; tel.: (499) 193-30-01
References
- Timchenko V.N. Infektsionnye bolezni u detei [Infectious Diseases in Children]. St. Petersburg, SpetsLit, 2006. 87–96p.
- Skerry C.M., Cassidy J.P. English K., Feunou-Feunou P., Locht C., Mahon B.P. Clin.Vac. Immunol. 2009; 16 (9): 1344–1351.
- Elachi,Sh., Holmstrom J., Gerdts V. TRENDS Microbiol. 2007; 15 (10): 462–468.
- Gustavsson O.E.A., Roken B.O., Serrander R. Folia Primatol. 1990; 55: 45–50.
- Huang С.С., Chen P.M., Kuo J.K. Chiu W.H., Lin S.T., Lin H.C., Lin Y.C. New Engl. J. Med. 1962; 266 (18): 107–111.
- Lapin B.A., Dzhikidze E.K., Shevtsova Z.V., Stasilevich Z.N. Modelirovanie infektsionnykh zabolevanii cheloveka na laboratornykh primatakh [Modeling of human infectious diseases on laboratory nonhuman primates]. Sochi, 2011. p.74–75.
- Culotta C.S., Harvey D.F., Gordon E.F. J. Pediatr. 1935; 6: 743–752.
- North E.A., Keogh E.V., Christie R., Anderson G. Aust. J. Exp. Biol. Med. Sci. 1940; 18: 125–130.
- Medkova A.Yu., Alyapkina Yu.S. i dr. Detskie infektsii = Children's infections. 2010; 4: 19–22.
- Oslopova V.N., Sabikova A.R., Abdulkhakova R.A. Klinicheskaya laboratornaya diagnostika. 3-e izd. [Clinical laboratory diagnostics. 3rd edition]. Мoscow: MEDpress-inform. 2005. 64 p.
- Nesterova I.V., Kolesnikova N.V. Metodicheskie rekomendatsii [Methodical recommendations]. Krasnodar. 1996; 9 (6/11): 22.
- Novikov P.D. Immunopatologiya, allergologiya, infektologiya = Immunopathology, allergology, infectology. 2000; 1: 62–66.
- Frimel' G.M. Immunologicheskie metody [Immunological methods.]. Мoscow: Meditsina. 1987. 82–88 p.
- Glants S. Mediko-biologicheskaya statistika [Biomedical Statistics]. Мoscow: Praktika. 1999.
- Warfel J.M., Beren J., Kelly V.K., Lee G., Merkel T.J. Inf. Immun. 2012; 80(4):1530-1536.
Supplementary files
