Serum Neutrophil Gelatinase Associated Lipocalin in Patients with Toxic Nephropathies. Prospective Study

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Abstract

Background. Drug induced kidney disorder (DIKD) is a frequent adverse event which contributes to morbidity and even incapacitation. Toxic nephropathy also is one of the pathological syndromes or complications in acute alcohol poisoning (AAP). Recent years experience shows the “insensitivity” of serum creatinine to the early stages of kidney damage. NGAL has been shown as a preferred marker of acute kidney damage in a variety of clinical settings. However, no similar studies of serum NGAL have been performed in patients with toxic nephropathy. The presence of markers discovers the possibility of earlier detection, timely treatment and prevention of disease progression to chronic kidney disease (CKD), which can improve the patient’s prognosis.

Aims — to study serum NGAL levels in patients with AAP and DIKD with clinically diagnosed nephropathy and without signs of kidney damage. The effect of glomerular filtration rate (GFR) on serum NGAL levels was studied.

Materials. This prospective cross-sectional study was conducted on the basis of the biochemical laboratory of the Karaganda Medical University in conjunction with the toxicological department of the Regional Medical Center (from January 2018 to October 2019). The study included 89 patients with AAP and 50 patients with DIKD. 25 healthy donors (control group) and 25 patients with CKD served as comparison groups. Serum NGAL levels were measured using a commercially available ELISA kit.

Results. We detected the increased serum NGAL level in both groups with drug-induced nephropathy and nephropathy, caused by AAP compared with the control group values (p < 0.01). However, there were no significant differences in NGAL level, depending on the type of toxic nephropathy. After ranking the GFR, it was revealed that GFR did not affect the serum NGAL level (F = 2.21; p = 0.12) and its increase was observed both at “reduced” and “increased” GFR relative to the control group values (p < 0.05).

Conclusions. The results of our study showed a multiple increase in the concentration of NGAL in serum not only in patients with toxic nephropathy, but also in patients with “increased” GFR, even in the absence of clinical and laboratory signs of impaired renal function.

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About the authors

Lyudmila A. Demidchik

Karaganda Medical University

Email: lusenok2008@mail.ru
ORCID iD: 0000-0002-8633-5164
SPIN-code: 9521-3615

PhD student, teacher staff, Biochemist

Kazakhstan, 40 Gogol str., 10000, Karaganda

Valentina V. Lee

Karaganda Medical University

Author for correspondence.
Email: vvv2705@mail.ru
ORCID iD: 0000-0002-5345-1994
SPIN-code: 9004-2845
Scopus Author ID: 57188624084
ResearcherId: P-1971-2017

PhD student, teacher staff, Nephrologist

Kazakhstan, 40 Gogol str., 10000, Karaganda

Dmitriy A. Klyuyev

Karaganda Medical University

Email: Klyuev@qmu.kz
ORCID iD: 0000-0003-2012-2227
SPIN-code: 8178-0220

MD, PhD

Kazakhstan, 40 Gogol str., 10000, Karaganda

Ryszhan Y. Bakirova

Karaganda Medical University

Email: R.Bakirova@qmu.kz
ORCID iD: 0000-0002-1592-8579
SPIN-code: 4926-6193

MD, PhD, Professor

Kazakhstan, 40 Gogol str., 10000, Karaganda

Vilen B. Molotov-Luchanskiy

Karaganda Medical University

Email: Molotov-luchanskiy@qmu.kz
ORCID iD: 0000-0001-8473-4375

MD, PhD, Professor

Kazakhstan, 40 Gogol str., 10000, Karaganda

Yelena V. Pozdnyakova

Karaganda Medical University

Email: Pozdnyakova@qmu.kz
ORCID iD: 0000-0002-3292-8757
SPIN-code: 3876-9653

PhD in Biology

Kazakhstan, 40 Gogol str., 10000, Karaganda

Irina V. Beinikova

Karaganda Medical University

Email: irena9898@mail.ru
ORCID iD: 0000-0001-5722-7650

Master of Medicine

Kazakhstan, 40 Gogol str., 10000, Karaganda

Semyon S. Bobyrev

Karaganda Medical University

Email: bobyrevsemyon@gmail.com
ORCID iD: 0000-0001-8171-3122

PhD Student

Kazakhstan, 40 Gogol str., 10000, Karaganda

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