Ischemic cardiomyopathy: blood monocytes and mediators of their differentiation

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  • Authors: Chumakova S.P.1, Shipulin V.M.1,2, Urazova O.I.1, Pogonchenkova D.A.1, Vince M.V.1, Pryakhin A.S.2, Kolobovnikova Y.V.1, Churina E.G.1,3, Novitskiy V.V.1
  • Affiliations:
    1. Siberian State Medical University
    2. Tomsk National Research Medical Center of the Russian Academy of Sciences, Cardiology Research Institute
    3. Tomsk State University (TSU)
  • Issue: Vol 74, No 6 (2019)
  • Pages: 396-404
  • URL:
  • DOI:
  • Cite item
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BACKGROUND: The three-year survival rate of patients with ischemic cardiomyopathy (ICMP) is 5−35 %; its pathogenesis is poorly understood. Violation of cytokine-dependent differentiation of monocytes/macrophages involved in atherogenesis may play an important role in the development of ICMP.

AIMS: To characterize the disorders of monocytes subpopulation composition and mediators spectrum of blood in patients with coronary heart disease (CHD), associated with the development of ICMP.

METHODS: A one-stage, clinical, controlled (case-control) study was conducted from February 2017 to December 2018. 45 patients with CHD (all men), who were in a cardiac surgery hospital, were examined before coronary bypass surgery: 19 people suffering from ICMP, and 26 people who do not suffer from ICMP, as well as 14 healthy men. In the blood of the examined individuals CD14++CD16-, CD14++CD16+, CD14+CD16++ and CD14+CD16- monocytes were determined with respect to all CD14-positive cells by flow cytometry, in blood plasma ― concentration of galectin 2 and 9, IL-4, IL-10, IFN-γ, M-CSF, HIF-1α by enzyme-linked immunosorbent assay (ELISA).

RESULTS: The development of the ICMP accompanied by deficiency of HIF-1α and CD14+CD16++ monocytes (0.037 [0.020; 0.045] ng/ml, p = 0.019, and 5.05 [4.08; 6.58] %, p = 0.011) in combination with an excess of IL-10 (30.05 [24.75; 33.50] ng/ml, p = 0.042) in the blood. It is shown in blood of patients without ischemic cardiomyopathy the increase in the content of CD14++CD16+ cells and lack of CD14+CD16- monocytes (25.27 [15.78; 31.39] %, p = 0.038, and 2.68 [2.63; 4.09] %, p = 0.027) at normal concentration of IL-10 and HIF-1α. In patients with CHD and ischemic cardiomyopathy and without ICMP in the blood the concentration of M-CSF and galectin-2 (2.00 [1.21; 3.24] pg/ml, p = 0.028, and 0.40 [0.12; 2.37] ng/ml, p = 0.004; 3.00 [1.90; 4.05] pg/ml, p = 0.003, and 3.20 [2.07; 4.00] ng/ml, p = 0.002, respectively) is reduced at normal content of galectin-9, and CD14++CD16- monocytes. IL-4 and IFN-γ in blood plasma are not determined (zero values).

CONCLUSIONS: The development of ICMP is associated with excess of IL-10 and HIF-1α deficiency, which is accompanied by inhibition of CD14+CD16++ monocytes maturation.

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About the authors

Svetlana P. Chumakova

Siberian State Medical University

Author for correspondence.
ORCID iD: 0000-0003-3468-6154
SPIN-code: 7536-2834

Russian Federation, 2, Moscowski Trakt, Tomsk, 634050

MD, PhD, Professor

Vladimir M. Shipulin

Siberian State Medical University; Tomsk National Research Medical Center of the Russian Academy of Sciences, Cardiology Research Institute

ORCID iD: 0000-0003-1956-0692

Russian Federation, 2, Moscowski Trakt, Tomsk, 634050; 5, Kooperativnyi s.s., Tomsk region, Tomsk, 634009

MD, PhD, Professor

Olga I. Urazova

Siberian State Medical University

ORCID iD: 0000-0002-9457-8879

Russian Federation, 2, Moscowski Trakt, Tomsk, 634050

MD, PhD, Professor

Darya A. Pogonchenkova

Siberian State Medical University

ORCID iD: 0000-0002-5903-3662
SPIN-code: 4141-9068

Russian Federation, 2, Moscowski Trakt, Tomsk, 634050

Maria V. Vince

Siberian State Medical University

SPIN-code: 8198-5676

Russian Federation, 2, Moscowski Trakt, Tomsk, 634050

ассистент кафедры патофизиологии

Andrew S. Pryakhin

Tomsk National Research Medical Center of the Russian Academy of Sciences, Cardiology Research Institute

ORCID iD: 0000-0003-0532-8091
SPIN-code: 9716-9356

Russian Federation, 5, Kooperativnyi s.s., Tomsk, 634009

аспирант отделения сердечно-сосудистой хирургии

Yulia V. Kolobovnikova

Siberian State Medical University

ORCID iD: 0000-0001-7156-2471
SPIN-code: 3638-1577

Russian Federation, 2, Moscowski Trakt, Tomsk, 634050

MD, PhD, Professor

Elena G. Churina

Siberian State Medical University; Tomsk State University (TSU)

ORCID iD: 0000-0002-8509-9921
SPIN-code: 9804-5093

Russian Federation, 2, Moscowski Trakt, Tomsk, 634050; 34a, Lenina prospect, Tomsk, 634050

д.м.н., профессор кафедры

Vyacheslav V. Novitskiy

Siberian State Medical University

ORCID iD: 0000-0002-9577-8370
SPIN-code: 7160-6881

Russian Federation, 2, Moscowski Trakt, Tomsk, 634050

MD, PhD, Professor


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