Features of Tissue Reaction in the Tenon Capsule in Progressive Myopia

Cover Page

Cite item


Background: Research actuality is determined by the first, the prevalence of refraction errors including progressive myopia among children secondly, high risk and tends to develop complications from the visual organ in refractive disorders.

Aims: To investigate tissue reactions occurring in the Tenon’s capsule with anomalies of refraction, including with progressive myopia.

Materials and methods: A one-step study of the Tenon’s capsule of 47 samples (25 with hyperopia and 22 with progressive myopia) was carried out. The material of the Tenon’s capsule was obtained during surgical treatment of strabismus and sclera strengthening operations with progressive myopia. The Tenon’s capsule was studied at different levels: tissue, cellular, subcellular. Fragments of Tenon’s capsule were stained with hematoxylin-eosin and picrofuchsin mixture by the method of van Gieson at the tissue level. This allowed obtaining a general picture of the morphology of Tenon’s capsule. Fragments of Tenon’s capsule were stained by toluidine blue in tetraborate sodium at the cellular level. This gave the opportunity to define the scope for ultratome and spend morphometry of cellular composition. A fragment of Tenon’s capsule was studied by transmission electron microscopy (TEM) at the subcellular level and was performed ultrastructural morphometry of fibroblasts, evaluation of the density of the collagen fibers.

Results: Were evaluated by qualitative and quantitative characteristics of the structure of Tenon’s capsule with two anomalies of refractions: progressive myopia and hyperopia: with progressive myopia in Tenon’s capsule, in contrast to hyperopia, the following number of fibroblasts (1.56±0.12 per 104 µm2), mast cells (0.08±0.02 per 104 µm2), adipocytes (0.01±0.001 per 104 µm2) were observed; ultrastructural features of fibroblasts were represented by such quantitative characteristics: the area of the fibroblast nucleus was 1.60±0.82 in µm2, the length of the karyolemma was 6.99±0.189 µm, the number of nucleoli was 0.17±0.015 per 1 µm2, the number of mitochondria and lysosome -2.05±0.14 per 1 µm2; 0.64±0.08 per 1 µm2, respectively); the density of collagen fiber was 28.72±4.18%, fibrillar fibrillation and fragmentation were recorded.

Conclusions: Hyperplasia of fibroblasts and their ultrastructures, mast cells, reduction in the level of adipocytes and the density of collagen fibrils ― these changes are features of the tissue reaction in the tenon capsule and reflect the adaptive nature of the processes occurring during progressive myopia. 

About the authors

Evgeny L. Kurenkov

South Ural state medical University of Ministry of health of Russia

Author for correspondence.
Email: KurenkovEL@mail.ru
ORCID iD: 0000-0002-3544-1143

MD, PhD, Professor

SPIN-cod: 2405-1197

Russian Federation

Vadim S. Rykun

South Ural state medical University of Ministry of health of Russia

Email: VSRykun@mail.ru
ORCID iD: 0000-0002-7287-0481

MD, PhD, Professor

SPIN-cod: 1219-0869

Russian Federation

Svetlana A. Gordeeva

Branch № 1 FGKU «354 Military clinical hospital» of the Ministry of defense

Email: mohnacheva87@mail.ru
ORCID iD: 0000-0002-5309-8318


SPIN-cod: 6312-7711

Russian Federation


  1. who.int [интернет]. ВОЗ. Информационный бюллетень. Нарушения зрения и слепота [доступ от 12.01.2019]. Доступ по ссылке https://www.who.int/ru/news-room/fact-sheets/detail/blindness-and-visual-impairment.
  2. Нероев В.В. Организация офтальмологической помощи населению Российской Федерации // Вестник офтальмологии. — 2014. — Т.130. — №6 — С. 8–12.
  3. Иомдина Е.Н., Тарутта Е.П. Современные направления фундаментальных исследований патогенеза прогрессирующей миопии // Вестник Российской академии медицинских наук. — 2014. — Т.69. — №3–4 — С. 44–49. doi: 10.15690/vramn.v69i3-4.994.
  4. Fernyhough ME, Hausman GJ, Guan LL, et al. Mature adipocytes may be a source of stem cells for tissue engineering. Biochem Biophys Res Commun. 2008;368(3):455–457. doi: 10.1016/j.bbrc.2008.01.113.
  5. Серов В.В., Шехтер А.Б. Соединительная ткань (функциональная морфология и общая патология). — М.: Медицина; 1981. — 312 с.
  6. Rubinchik E, Levi-Schaffer F. Mast cells and fibroblasts: two interacting cells. Int J Clin Lab Res. 1994;24(3):139–142. doi: 10.1007/bf02592443.
  7. Dvorak AM, Mitsui H, Ishizaka T. Stimulation of partial development of human mast cells by supernatant fluid from mouse fibroblast cultures. Clin Exp Allergy. 1994;24(7):649–659. doi: 10.1111/j.1365-2222.1994.tb00969.x.
  8. Саркисов Д.С. Структурные основы надежности биологических систем // Архив патологии. — 1994. — Т.56. — №5 — С. 4–8.
  9. Куренков Е.Л. Морфологическая характеристика полиповидных образований желудка и фонового хронического гастрита // Российский журнал гастроэнтерологии, гепатологии, колопроктологии. — 2000. — Т.10. — №2 — С. 18–25.
  10. Куренков Е.Л., Коваленко В.Л. Активность ядрышковых организаторов слизь продуцирующего эпителия в морфогенезе приобретенных эпителиальных полипов желудка // Российский журнал гастроэнтерологии, гепатологии и колопроктологии. — 2004. — Т.14. — №5 — С. 30–34.
  11. McBrien NA, Cornell LM, Gentle A. Structural and ultrastructural changes to the sclera in a mammalian model of high myopia. Invest Ophthalmol Vis Sci. 2001;42(10):2179–2187.
  12. Иомдина Е.Н., Тарутта Е.П., Игнатьева Т.Ю., и др. Структурно-морфологические особенности коллагена теноновой капсулы глаза при гиперметропии и миопии: сб. науч. тр. Т.1. — М.; 2009. — С. 370–374.
  13. Ульянова Н.А., Думброва Н.Е., Молчанюк Н.И. Морфологические изменения склеры при моделировании миопии // Морфологiя. — 2014. — Т.8. — №2 — С. 72–76.
  14. Николаева Т.Э. Гистологические, гистохимические и электронно-микроскопические исследования склеры при миопии: Автореф. дис. ... канд. мед. наук. — М.; 1974. — 19 с.

Copyright (c) 2019 "Paediatrician" Publishers LLC

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies