Status Indicators of Lipid Peroxidation and Endogenous Intoxication in Lung Cancer Patients

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Background: Problems of optimization diagnosis methods and prognosis for lung cancer remain unsolved. Lung cancer occupied the leading positions among cancer diseases.

Aims: Establishing change patterns in the parameters of endogenous intoxication and lipid peroxidation in the saliva of patients with lung cancer depending on the histologic type of tumor.

Materials and methods: The case-control study enrolled 516 men, who were divided into 3 groups: main (lung cancer, n=256), comparison group (non-malignant lung diseases, n=60), and control group (relatively healthy, n=200). Questioning and biochemical saliva study were carried out to all participants. Patients of the main group and the comparison group were hospitalized for surgical treatment, after which underwent the histological verification of the diagnosis. We used the spectrophotometric methods of investigation of parameters of lipid peroxidation and endogenous intoxication. Between-group differences were evaluated by nonparametric tests.

Results: Malondialdehyde as a product of lipid peroxidation is a little informative result. For more information it is necessary to determine the individual fractions of middle toxins count distribution ratio 280/254 nm, as well as to take into account the level of conjugated diene, triene conjugates, and Schiff bases. The following changes are observed at the transition from the control group to the comparison group, and then to the main: increased levels of triene conjugates and Schiff bases, as well as malondialdehyde. At the same time we detected the reduction in the level of diene conjugates, which confirms the fact of the increase in the oxidative stress process associated with benign diseases and lung cancer. In addition, there is a decrease in the content of individual fractions of middle toxins, but 280/254 nm partition coefficient growth is observed.

Conclusions: The findings support the hypothesis of the association processes of lipid peroxidation and endogenous intoxication with the development of lung cancer. It confirmed the dependence of these parameters on the histological type of tumor, the presence / absence and the degree of prevalence of remote and regional metastasis.

About the authors

L. V. Belskaya

Omsk State Technical University, Omsk, Russian Federation

Author for correspondence.
ORCID iD: 0000-0002-6147-4854

кандидат химических наук, директор по науке ООО «ХимСервис», доцент кафедры химической технологии и биотехнологии Омского государственного технического университета Адрес: 644050, Омск, Проспект Мира, д. 11, тел.: +7 (913) 641-35-77

Russian Federation

V. K. Kosenok

ChemService, Omsk, Russian Federation

Omsk State Medical University, Omsk, Russian Federation

ORCID iD: 0000-0002-2072-2460

доктор медицинских наук, профессор, академик РАМТН, заведующий кафедрой онкологии с курсом лучевой терапии Омского государственного медицинского университета Адрес: 644013, Омск, ул. Завертяева, д. 9, корп. 1, тел.: +7 (3812) 60-17-46

Z. Massard

University Hospital of Strasbourg, Strasbourg, France


профессор, главный онколог Страсбургского университета, заведующий отделением торакальной хирургии и трансплантологии, академик РАМН, член Американской ассоциации торакальной хирургии Адрес: 67091, Страсбург, ул. Порт дё Лопиталь, ВР 426

A. A. Zav’yalov

Federal State Budgetary Scientifi c Institution «Tomsk Саnсеr Rеsеаrсh Institute», Tomsk, Russian Federation


доктор медицинских наук, профессор, ведущий научный сотрудник Томского национального исследовательского медицинского центра РАН Адрес: 634050, Томск, пер. Кооперативный, д. 5, тел.: +7 (3822) 41-80-89


  1. Mil’ EM, Gurevich SM, Kozachenko AI, et al. Effect of smoking and tumor progress on the contents of key proteins of apoptosis and activity of antioxidant enzymes in blood. Biol Bull Russ Acad Sci. 2012;39(1):15–21. doi: 10.1134/s1062359011060094.
  2. Sesti F, Tsitsilonis OE, Kotsinas A, Trougakos IP. Oxidative stressmediated biomolecular damage and inflammation in tumorigenesis. In Vivo. 2012;26(3):395–402.
  3. Dayem AA, Choi HY, Kim JH, Cho SG. Role of oxidative stress in stem, cancer, and cancer stem cells. Cancers (Basel). 2010;2(2):859–884. doi: 10.3390/cancers2020859.
  4. Малахова М.Я. Эндогенная интоксикация как отражение компенсаторной перестройки обменных процессов в организме // Эфферентная терапия. — 2000. — Т.6. — №4. — С. 3–14. [Malakhova MYa. Endogennaya intoksikatsiya kak otrazhenie kompensatornoi perestroiki obmennykh protsessov v organizme. Efferentnaya terapiya. 2000;6(4):3–14. (In Russ).]
  5. Halliwell B, Gutteridge JM. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J. 1984;219(1):1–14. doi: 10.1042/bj2190001.
  6. Чеснокова Н.П., Барсуков В.Ю., Понукалина Е.В., Агабеков А.И. Закономерности изменений процессов свободно-радикальной дестабилизации биологических мембран при аденокарциноме восходящего отдела ободочной кишки, их роль в развитии опухолевой прогрессии // Фундаментальные исследования. — 2015. — №1. — С. 164–168. [Chesnokova NP, Barsukov VY, Ponukalina EV, Agabekov AI. Regularities of changes in free radical destabilization processes of biological membranes in cases of colon ascendens adenocarcinoma and the role of such regularities in neoplastic proliferation development. Fundamental’nye issledovaniya. 2015;(1):164– 168. (In Russ).]
  7. Панкова О.В., Перельмутер В.М., Савенкова О.В. Характеристика экспрессии маркеров пролиферации и регуляции апоптоза в зависимости от характера дисрегенераторных изменений в эпителии бронхов при плоскоклеточном раке легкого // Сибирский онкологический журнал. — 2010. — Т.41. — №5. — С. 36–41. [Pankova OV, Perelmuter VM, Savenkova OV. Characteristics of prolifeartion marker expression and apoptosis regulation depending on the character of disregenerator changes in bronchial epithelium of patients with squamous cell lung cancer. Sibirskii onkologicheskii zhurnal. 2010;41(5):36–41. (In Russ).]
  8. Wong DT. Salivary diagnostics. New York: Wiley-Blackwell; 2008. 320 p.
  9. Malathi N, Mythili S, Vasanthi HR. Salivary diagnostics: a brief review. ISRN Dent. 2014;2014:158786. doi: 10.1155/2014/158786.
  10. Miller CS, Foley JD, Bailey AL, et al. Current developments in salivary diagnostics. Biomark Med. 2010;4(1):171–189. doi: 10.2217/bmm.09.68.
  11. Arunkumar S, Arunkumar JS, Krishna NB, Shakunthala GK. Developments in diagnostic applications of saliva in oral and systemic diseases — A comprehensive review. Journal of Scientific and Innovative Research. 2014;3(3):372−387.
  12. Nunes LA, Mussavira S, Bindhu OS. Clinical and diagnostic utility of saliva as a non-invasive diagnostic fluid: a systematic review. Biochem Med (Zagreb). 2015;25(2):177–192. doi: 10.11613/ BM.2015.018.
  13. Al-Rawi NH. Salivary lipid peroxidation and lipid profile levels in patients with recent ischemic stroke. J Int Dent Med Res. 2010;3(2):57–64.
  14. Nguyen TT, Ngo LQ, Promsudthi A, Surarit R. Salivary Lipid Peroxidation in Patients With Generalized Chronic Periodontitis and Acute Coronary Syndrome. J Periodontol. 2016;87(2):134–141. doi: 10.1902/jop.2015.150353.
  15. Sobaniec H, Sobaniec W, Sendrowski K, et al. Antioxidant activity of blood serum and saliva in patients with periodontal disease treated due to epilepsy. Adv Med Sci. 2007;52(Suppl. 1):204–206.
  16. Guentsch A, Preshaw PM, Bremer-Streck S, et al. Lipid peroxidation and antioxidant activity in saliva of periodontitis patients: effect of smoking and periodontal treatment. Clin Oral Investig. 2008;12(4):345–352. doi: 10.1007/s00784-008-0202-z.
  17. Niki E. Lipid peroxidation products as oxidative stress biomarkers. Biofactors. 2008;34(2):171–180. doi: 10.1002/biof.5520340208.
  18. Demirtas M, Senel U, Yuksel S, Yuksel M. A comparison of the generation of free radicals in saliva of active and passive smokers. Turk J Med Sci. 2014;44(2):208–211. doi: 10.3906/sag-1203-72.
  19. Rai B, Kharb S, Jain R, Anand SC. Salivary lipid peroxidation product malondialdehyde in precancer and cancer. Redox Rep. 2007;12(3):163–164. doi: 10.1179/135100007x200245.
  20. Shivashankara AR, Kavya PM. Salivary total protein, sialic acid, lipid peroxidation and glutathione in oral squamous cell carcinoma. Biomedical Research (Aligarh, India). 2011;22(3):355–359.
  21. Shetty SR, Babu S, Kumari S, et al. Status of salivary lipid peroxidation in oral cancer and precancer. Indian J Med Paediatr Oncol. 2014;35(2):156–158. doi: 10.4103/0971-5851.138990.
  22. Chandra Pr, Sharma D, Gupta A, Sowmya MK. An In-vitro study of lipid peroxidation, vitamin E and vitamin C levels in saliva of oral precancerous patients in District Hapur of Uttar Pradesh. IJBAR. 2013;4(4):233−236. doi: 10.7439/ijbar.v4i4.330.
  23. Hegde N, Kumari SN, Hegde MN, et al. Lipid peroxidation and vitamin C level in saliva of oral precancerous patients — an in-vitro study. Res J Pharm Biol Chem Sci. 2011;2(2):13−18.
  24. Волчегорский И.А., Налимов А.Г., Яровинский Б.Г. Сопоставление различных подходов к определению продуктов в гептанизопропанольных экстрактах крови // Вопросы медицинской химии. — 1989. — Т.35. — №1. — С. 127–131. [Volchegorskii IA, Nalimov AG, Yarovinskii BG. Sopostavlenie razlichnykh podkhodov k opredeleniyu produktov v geptan-izopropanol’nykh ekstraktakh krovi. Vopr Med Khim. 1989;35(1):127–131. (In Russ).]
  25. Гаврилов В.Б., Гаврилова А.Р., Мажуль Л.М. Анализ методов определения продуктов перекисного окисления липидов в сыворотке крови по тесту с тиобарбитуровой кислотой // Вопросы медицинской химии. — 1987. — Т.33. — №1. — С. 118–122. [Gavrilov VB, Gavrilova AR, Mazhul’ LM. Analiz metodov opredeleniya produktov perekisnogo okisleniya lipidov v syvorotke krovi po testu s tiobarbiturovoi kislotoi. Vopr Med Khim. 1987;33(1):118–122. (In Russ).]
  26. Гаврилов В.Б., Бидула М.М., Фурманчук Д.А., и др. Оценка интоксикации организма по нарушению баланса между накоплением и связыванием токсинов в плазме // Клиническая лабораторная диагностика. — 1999. — №2. — С. 13–17. [Gavrilov VB, Bidula MM, Furmanchuk DA, et al. Otsenka intoksikatsii organizma po narusheniyu balansa mezhdu nakopleniem i svyazyvaniem toksinov v plazme. Klin Lab Diagn. 1999;(2):13–17. (In Russ).]
  27. Hultqvist M, Hegbrant J, Nilsson-Thorell C, et al. Plasma concentrations of vitamin C, vitamin E and/or malondialdehyde as markers of oxygen free radical production during hemodialysis. Clin Nephrol. 1997;47(1):37–46.
  28. Sato EF, Choudhury T, Nishikawa T, Inoue M. Dynamic aspect of reactive oxygen and nitric oxide in oral cavity. J Clin Biochem Nutr. 2008;42(1):8–13. doi: 10.3164/jcbn.2008002.
  29. Halliwell B. Free radicals and antioxidants: updating a personal view. Nutr Rev. 2012;70(5):257– 265. doi: 10.1111/j.1753-4887.2012.00476.x.
  30. Sotgia F, Martinez-Outschoorn UE, Lisanti MP. Mitochondrial oxidative stress drives tumor progression and metastasis: should we use antioxidants as a key component of cancer treatment and prevention? BMC Med. 2011;9:62. doi: 10.1186/1741-7015-9-62.
  31. Nomori H, Watanabe K, Ohtsuka T, et al. The size of metastatic foci and lymph nodes yielding false-negative and false-positive lymph node staging with positron emission tomography in patients with lung cancer. J Thorac Cardiovasc Surg. 2004;127(4):1087–1092. doi: 10.1016/j.jtcvs.2003.08.010.
  32. Лапешин П.В., Савченко А.А., Дыхно Ю.А., и др. Особенности фенотипического состава лимфоцитов крови и лимфоузлов у больных аденокарциномой и плоскоклеточным раком легкого // Сибирский онкологический журнал. — 2005. — Т.14. — №2. — С. 34–38. [Lapeshin PV, Savchenko AA, Dichno UA, et al. Phenotypic composition of blood lymphocytes and lymph nodes in patients witadenocarcinoma and squamous cell carcinoma of the lun. Sibirskii onkologicheskii zhurnal. 2005;14(2):34–38. (In Russ).]
  33. Багиров Р.Р., Полоцкий Б.Е. Рак легкого у больных молодого возраста // Российский биотерапевтический журнал. — 2009. — Т.8. — №4. — С. 79–84. [Bagirov RR, Polockiy BE. Lung cancer in young patients. Rossiiskii bioterapevticheskii zhurnal. 2009;8(4):79– 84. (In Russ).]
  34. Савченко А.А., Лапешин П.В., Дыхно Ю.А. Состояние иммунной системы и метаболизм здоровых и опухолевых клеток легочной ткани у больных немелкоклеточным раком легкого в зависимости от метастазирования // Российский биотерапевтический журнал. — 2005. — Т.4. — №2. — С. 106–112. [Savchenko AA, Lapeshin PV, Dichno UA. СImmune system state and metabolic activity in normal and tumor lung cells tissue in patients with and without metastasis in root lymph nodes. Rossiiskii bioterapevticheskii zhurnal. 2005;4(2):106–112. (In Russ).]
  35. Park EM, Ramnath N, Yang GY, et al. High superoxide dismutase and low glutathione peroxidase activities in red blood cells predict susceptibility of lung cancer patients to radiation pneumonitis. Free Radic Biol Med. 2007;42(2):280–287. doi: 10.1016/j.freeradbiomed.2006.10.044.
  36. Туманский В.А., Шевченко А.И., Колесник А.П., и др. Показатели пролиферативной активности опухоли у больных ранними стадиями немелкоклеточного рака легкого // Патология. — 2010. — Т.7. — №2. — С. 81–84. [Tumansky VA, Shevchenko AI, Kolesnik AP, et al. Proliferative activity of the tumors of patients with early stage non-small cell lung cancer. Patologiya. 2010;7(2):81–84. (In Russ).]
  37. Pankiewicz W, Minarowski L, Niklinska W, et al. Immunohistochemical markers of cancerogenesis in the lung. Folia Histochem Cytobiol. 2007;45(2):65–74.

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