GLUTATHIONE-DEPENDENT ENZYMES AND GLUTATHIONE IN INFERTILITY OF MEN WITH DIFFERENT BODY MASS

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Abstract


Objective: Our aim was to investigate the glutathione-disulfide system of antioxidant defense in men with different body mass index and infertility. Methods: 60 men with infertility were examined — 30 with normal weight and 30 with overweight. 30 fertile men were included in the control group. Hemolysate made from red blood cells was used as a material for study. The level of reduced (GSH) and oxidized glutathione (GSSG), and glutathione activity was determined by enzyme spectrofluorophotometer SHIMADZU-1501 (Japan). Results: In infertile men with normal body weight decrease of GSH to 15.35% was combined with a decrease in the activity of enzymes — glutathione reductase (GR) to 53.41%, glutathione peroxidase (GPO) to 51.44%, and increased activity of glutathione-S-transferaze (GST) to 31.52% as compared with the control group. In the group of infertile men with overweight a decrease in the activity of GR, 56.77% and 56.22% at the GPO on the background of a stable level of GST was detected in comparison with fertile men. The concentration of GSH in infertile group decreased by 20.08%, and the concentration of GSSG increased by 20.38%. Conclusion: It has been established that imbalance in the glutathione system is the most prominent feature in men with infertility and overweight as compared to infertile men with normal body weight.


L. I. Kolesnikova

Scientifi c Center for Family Health and Human Reproduction Problems, Irkutsk, Russian Federation

Author for correspondence.
Email: iphr@sbamsr.irk.ru

Russian Federation доктор медицинских наук, профессор, член-корр. РАН, директор НЦПЗСРЧ Адрес: 664003, Иркутск, ул. Тимирязева, д. 16, тел.: +7 (3952) 20-76-36

O. A. Vanteeva

Scientifi c Center for Family Health and Human Reproduction Problems, Irkutsk, Russian Federation

Email: iphr@sbamsr.irk.ru

Russian Federation кандидат биологических наук, младший научный сотрудник лаборатории патофизиологии репродукции НЦПЗСРЧ
Адрес: 664003, Иркутск, ул. Тимирязева, д. 16, тел.: +7 (3952) 20-76-36

N. A. Kurashova

Scientifi c Center for Family Health and Human Reproduction Problems, Irkutsk, Russian Federation

Email: iphr@sbamsr.irk.ru

Russian Federation кандидат биологических наук, научный сотрудник лаборатории патофизиологии репродукции НЦПЗСРЧ
Адрес: 664003, Иркутск, ул. Тимирязева, д.16, тел.: +7 (3952) 20-76-36

B. G. Dashiev

Republican Perinatal Center, Ulan-ude, the Republic of Buryatia, Russian Federation

Email: bairdashiev@mail.ru

Russian Federation кандидат медицинских наук, врач уролог-андролог Республиканского перинатального центра
Адрес: 670002, Республика Бурятия, Улан-Удэ, ул. Комсомольская, д. 1/Б, тел.: +7 (3012) 55-36-23

  1. Vanteeva O.A., Kurashova N.A., Dashiev B.G. Analysis of the state of the antioxidant defense system in men with infertility of different ethnic groups. Byulleten' VSNTs SO RAMN = Bulletin of ESSC SD RAMS. 2013; 4 (92): 102–105.
  2. Kolesnikova L.I., Darenskaya M.A., Grebenkina L.A. Labygina A.V., Suturina L.V., Dolgikh M.I., Shiphineeva T.I., Darzhaev Z.Yu., Tsyrenov T.B., Rinchindorzhieva M.P. Activity of lipid peroxidation in infertile women from different populations. Bull. Exp. Biol. Med. 2012; 154 (2): 203–205.
  3. Kurashova N.A. Evaluation of the reproductive potential of the male population. Byulleten' VSNTs SO RAMN = Bulletin of ESSC SD RAMS. 2014; 2 (96): 104–109.
  4. Kolesnikova L.I., Grebenkina L.A., Darenskaya M.A., Vlasov B.Ya. Oxidative stress as a nonspecific pathogenetic link reproductive disorders. Byulleten' VSNTs SO RAMN = Bulletin of ESSC SD RAMS. 2012; 32 (1): 58–66.
  5. Sallmen M., Sandler D.P., Hoppin J.A., Blair A., Baird D.D. Reduced Fertility Among Overweight and Obese Men. Epidemiology. 2006; 17 (5): 520–523.
  6. Vanteeva O.A., Kurashova N.A., Dashiev B.G. Lipid peroxidation in men with infertility. Byulleten' VSNTs SO RAMN = Bulletin of ESSC SD RAMS. 2014; 2 (96): 12–15.
  7. Khlyakina O.V. Condition of the reproductive system of men with metabolic syndrome living in ecologically unfavorable region. Vestnik vosstanovitel'noi meditsiny = Bulletin of regenerative medicine. 2011; 4: 63–66.
  8. Brody S.A. Male infertility and oxidative stress: the role of diet, lifestyle and dietary supplements. Andrologiya i genital'naya khirurgiya = Andrology and genital surgery. 2014; 3: 33–41.
  9. Hissin P.J., Hilf R. Fluometric method for determination of oxidizedand reduced glutathione intissues. Ann. Biochem. 1976; 74 (1): 214–226.
  10. Karpishchenko A.I. Meditsinskie laboratornye tekhnologii. Spravochnik [Medical Laboratory Technologies. Reference Book]. St. Petersburg, Intermedika, 2002. 600 p.
  11. Rukovodstvo VOZ po laboratornomu issledovaniyu eyakulyata cheloveka i vzaimodeistviya spermatozoidov s tservikal'noi sliz'yu. 4-e izd. Pod red. L.F. Kurilo [WHO guidelines for laboratory testing of human semen and sperm interaction with cervical mucus. 4th ed. Ed. by L.F. Kurilo]. Moscow, Med-Press, 2001. 144 p.
  12. Kolesnikova L.I., Osipova E.V., Grebenkina L.A., Vlasov B.Ya. Oxidative stress as an inducer of pathological disorders in humans. Byulleten' VSNTs SO RAMN = Bulletin of ESSC SD RAMS. 2009; S2: 48–49.
  13. Lyu B.N., Ismailov S.B., Lyu M.B. The state of the cytoskeleton: the relationship with the "oxygen-peroxide" effects in normal and pathological conditions in cellular apoptosis. Biomeditsinskaya khimiya = Biomedical chemistry. 2008; 1: 58–77.
  14. Cord J.M., Fridovich I. Superoxide dismutase. An enzymic function for erytrocuprein (hemocuprein). Biol. Chem. 2000; 244: 6049–6055.
  15. Miao L., Clair D.K. Regulation of superoxide dismutase genes: Implications in disease. Free Radic. Biol. Med. 2009; 47: 344–350.
  16. Tolpygina O.A. The role of glutathione in the antioxidant defense system. Byulleten' VSNTs SO RAMN = Bulletin of ESSC SD RAMS. 2012; 2–2: 178–180.
  17. Bednarek-Tupikowska G., Tupikowski K., Bidzińska B., Bohdanowicz-Pawlak A., Antonowicz-Juchniewicz J., Kosowska B., Milewicz A. Serum lipid peroxides and total antioxidant status in postmenopausal women on hormone replacement therapy. Gynecol. Endocrinol. 2004; 19: 57–63.
  18. Klebanoff S.J. Mieloperoxidase: role in neutrophil-mediated toxicity. Mol. Biol. Infec. Dis. 2006; 14: 283–289.
  19. Efimtseva E.A., Chelpanova T.I. Paraoxonase: molecular genetic aspects and clinical implications. Uspekhi sovremennoi biologii = Achievements of modern biology. 2012; 132: 282–296.
  20. Sharma R., Yang Y., Sharma A., Awasthi S., Awasthi Y.C. Antioxidant role of glutathione-S-transferases: protection against oxidant toxicity and regulation of stress-mediated apoptosis. Antioxid. Redox. Signal. 2006; 6: 289–300.
  21. Agarkov A.A., Popova T.N., Verevkin A.N., Matasova L.V. Activity of glutathione antioxidant system and NADPH-generating enzymes in the blood serum of rats with Type 2 diabetes and the effects of drugs, correcting melatonin levels. Byulleten' eksperimental'noi biologii i meditsiny = Bulletin of experimental biology and medicine. 2014; 157 (2): 158–162.

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