GLUTATHIONE-DEPENDENT ENZYMES AND GLUTATHIONE IN INFERTILITY OF MEN WITH DIFFERENT BODY MASS

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Abstract

Objective: Our aim was to investigate the glutathione-disulfide system of antioxidant defense in men with different body mass index and infertility. Methods: 60 men with infertility were examined — 30 with normal weight and 30 with overweight. 30 fertile men were included in the control group. Hemolysate made from red blood cells was used as a material for study. The level of reduced (GSH) and oxidized glutathione (GSSG), and glutathione activity was determined by enzyme spectrofluorophotometer SHIMADZU-1501 (Japan). Results: In infertile men with normal body weight decrease of GSH to 15.35% was combined with a decrease in the activity of enzymes — glutathione reductase (GR) to 53.41%, glutathione peroxidase (GPO) to 51.44%, and increased activity of glutathione-S-transferaze (GST) to 31.52% as compared with the control group. In the group of infertile men with overweight a decrease in the activity of GR, 56.77% and 56.22% at the GPO on the background of a stable level of GST was detected in comparison with fertile men. The concentration of GSH in infertile group decreased by 20.08%, and the concentration of GSSG increased by 20.38%. Conclusion: It has been established that imbalance in the glutathione system is the most prominent feature in men with infertility and overweight as compared to infertile men with normal body weight.

About the authors

L. I. Kolesnikova

Scientifi c Center for Family Health and Human Reproduction Problems, Irkutsk, Russian Federation

Author for correspondence.
Email: iphr@sbamsr.irk.ru
доктор медицинских наук, профессор, член-корр. РАН, директор НЦПЗСРЧ Адрес: 664003, Иркутск, ул. Тимирязева, д. 16, тел.: +7 (3952) 20-76-36 Россия

O. A. Vanteeva

Scientifi c Center for Family Health and Human Reproduction Problems, Irkutsk, Russian Federation

Email: iphr@sbamsr.irk.ru
кандидат биологических наук, младший научный сотрудник лаборатории патофизиологии репродукции НЦПЗСРЧ
Адрес: 664003, Иркутск, ул. Тимирязева, д. 16, тел.: +7 (3952) 20-76-36 Россия

N. A. Kurashova

Scientifi c Center for Family Health and Human Reproduction Problems, Irkutsk, Russian Federation

Email: iphr@sbamsr.irk.ru
кандидат биологических наук, научный сотрудник лаборатории патофизиологии репродукции НЦПЗСРЧ
Адрес: 664003, Иркутск, ул. Тимирязева, д.16, тел.: +7 (3952) 20-76-36 Россия

B. G. Dashiev

Republican Perinatal Center, Ulan-ude, the Republic of Buryatia, Russian Federation

Email: bairdashiev@mail.ru
кандидат медицинских наук, врач уролог-андролог Республиканского перинатального центра
Адрес: 670002, Республика Бурятия, Улан-Удэ, ул. Комсомольская, д. 1/Б, тел.: +7 (3012) 55-36-23 Россия

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