In vitro Cultivation of Functioning Passaged Ciliated Epithelium for Trachea Tissue Engineering

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Abstract


Background: Currently all tissue engineered trachea transplants had no ciliated epithelium until transplantation, and long-term temporary lack of mucociliary clearance leads to patients’ condition decline and reduced life quality in postoperative period. So, the need for a better cultivation method and studying ciliated epithelium growth characteristics in cell culture increased rapidly.

Objective: The aim of our study was to investigate cultivation of functionally complete passaged ciliated epithelium for trachea tissue engineering.

Methods: Human ciliated epithelium isolated from intraoperative bioptate was used for culturing in the special complex medium with morphological and functional characteristics evaluation.

Results: Ciliated epithelial cell-groups were obtained by culturing in the special complex medium. Generated cell-groups had ciliary activity and showed well-coordinated movement with functional characteristics similar to native epithelial tissue. The basic parameters of cell-activity were studied.

Conclusion: Thus our study provides a new insight for the problem of ciliated epithelium in vitro culturing as well as developing the optimal laboratory method.


D. S. Baranovsky

Sechenov First Moscow State Medical University, Moscow, Russian Federation

Author for correspondence.
Email: dennissb@mail.com

Russian Federation

младший научный сотрудник НИИ молекулярной медицины Первого МГМУ им. И.М. Сеченова Адрес: 119992, Москва, ул. Трубецкая, д. 8, стр. 2, тел.: +7 (495) 609-14-00

A. V. Lyundup

Sechenov First Moscow State Medical University, Moscow, Russian Federation

Email: lyundup@gmail.com

Russian Federation

кандидат медицинских наук, заведующий отделом биомедицинских исследований НИИ молекулярной медицины Первого МГМУ им. И.М. Сеченова Адрес: 119992, Москва, ул. Трубецкая, д. 8, стр. 2, тел.: +7 (495) 609-14-00

V. D. Parshin

Sechenov First Moscow State Medical University, Moscow, Russian Federation

Email: vdparshin@yandex.ru

Russian Federation

доктор медицинских наук, профессор, заведующий отделением торакальной хирургии Университетской клинической больницы № 1 Первого МГМУ им. И.М. Сеченова Адрес: 119992, Москва, ул. Трубецкая, д. 8, стр. 2, тел.: +7 (495) 609-14-00

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