Comparison Study of Oropharyngeal Microbiota in Case of Bronchial Asthma and Chronic Obstructive Pulmonary Disease in Different Severity Levels

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  • Authors: Ogorodova L.M.1, Fedosenko S.V.1, Popenko A.S.2, Petrov V.A.1, Tyakht A.V.2, Saltykova I.V.1, Deyev I.A.1, Kulikov E.S.1, Kirillova N.A.1, Govorun V.M.2,3, Kostryukova E.S.2,3
  • Affiliations:
    1. Siberian State Medical University
    2. Scientific Research Institute of Physical-Chemical Medicine
    3. Kazan Federal University
  • Issue: Vol 70, No 6 (2015)
  • Pages: 669-678
  • Section: MICROBIOLOGY: CURRENT ISSUES
  • URL: https://vestnikramn.spr-journal.ru/jour/article/view/532
  • DOI: https://doi.org/10.15690/vramn532
  • Cite item

Abstract


BackgraundThe result of comparative study of oropharyngeal microbiota taxonomic composition in patients with different severity level of bronchial asthma (BA) and chronic obstructive pulmonary disease (COPD) is presented in this paper. 

Aims: To compare oropharyngeal microbiota composition in case of bronchial asthma and chronic obstructive pulmonary disease in different severity levels. 

Metods138 patients, 50 with BA and 88 with COPD were studied. For each patient was collected anamnesis vitae, swab from the back of the throat and performed physical examination. High-throughput 16S ribosomal RNA gene sequencing and bioinformatic analysis was employed to characterize the microbial communities. 

ResultsAs a result of the study was found a number of differences on various taxonomic levels in microbiota’s composition within group of patients with different severity level of BA and group of patients with different severity level of COPD and between those groups. COPD patients with GOLD 1–2 in comparison with GOLD 3–4 patiens are marked by prevalence of species Brevibacterium aureum, genus Scardovia, Coprococcus, Haemophilus, Moryella, Dialister, Paludibacter and decrease of Prevotella melaninogenica species. BA patients with severe uncontrolled asthma in comparison with patients which have mild persistent asthma are marked by decrease of Prevotella and increase of species Bifidobacterium longum, Prevotella nanceiensis, Neisseria cinerea, Aggregatibacter segnis and genus Odoribacter, Alloiococcus, Lactobacillus, Megasphaera, Parvimonas, Sneathia. Patient’s microbiota in BA group in comparison with COPD group is characterized by the prevalence of Prevotella melaninogenica and genus Selenomonas, Granulicatella и Gemella, and decrease of Prevotella nigrescens, Haemophilus influenza and genus Aggregatibacter, Alloiococcus, Catonella, Mycoplasma, Peptoniphilus и Sediminibacterium. There are no differences between microbiota composition in case of severe uncontrolled BA and very severe COPD. 

ConclusionLack of differences in oropharyngeal microbiota taxonomic composition between patients with severe uncontrolled BA and very severe COPD allow us to suggest a similarity of bronchopulmonary system condition in case of diseases' severe stages.


Ludmila Mikhailovna Ogorodova

Siberian State Medical University

Email: lm-ogorodova@mail.ru

Russian Federation доктор медицинских наук, профессор, член-корреспондент РАМН, заведующая кафедрой факультетской педиатрии с курсом детских болезней лечебного факультета ГБОУ ВПО «Сибирский государственный медицинский университет» Минздрава России, заместитель министра науки и образования Российской Федерации

Sergey Vyacheslavovich Fedosenko

Siberian State Medical University

Author for correspondence.
Email: s-fedosenko@mail.ru

Russian Federation кандидат медицинских наук, докторант кафедры госпитальной терапии с курсом физической реабилитации и спортивной медицины ГБОУ ВПО «Сибирский государственный медицинский университет» Минздрава России

Anna Sergeevna Popenko

Scientific Research Institute of Physical-Chemical Medicine

Email: kirika77@gmail.com

Russian Federation лаборант лаборатории биоинформатики ФГБУН «Научно-исследовательский институт физико-химической медицины» ФМБА России

Vyacheslav Alekseevich Petrov

Siberian State Medical University

Email: vyacheslav.a.petrov@mail.ru

Russian Federation

младший научный сотрудник центральной научно-исследовательской лаборатории ГБОУ ВПО «Сибирский государственный медицинский университет» Минздрава России 

Alexandr Viktorovich Tyakht

Scientific Research Institute of Physical-Chemical Medicine

Email: at@niifhm.ru

Russian Federation кандидат биологических наук, младший научный сотрудник лаборатории биоинформатики ФГБУН «Научно-исследовательский институт физико-химической медицины» ФМБА России

Irina Vladimirovna Saltykova

Siberian State Medical University

Email: ira.salticova@mail.ru

Russian Federation кандидат медицинских наук, научный сотрудник Центральной научно-исследовательской лаборатории ГБОУ ВПО «Сибирский государственный медицинский университет» Минздрава России

Ivan Anatolievich Deyev

Siberian State Medical University

Email: ivandeyev@yandex.ru

Russian Federation доктор медицинских наук, ассистент кафедры факультетской педиатрии с курсом детских болезней лечебного факультета ГБОУ ВПО «Сибирский государственный медицинский университет» Минздрава России

Evgeny Sergeevich Kulikov

Siberian State Medical University

Email: evgeny.s.kulikov@gmail.com

Russian Federation доктор медицинских наук, доцент кафедры общей врачебной практики и поликлинической терапии ГБОУ ВПО «Сибирский государственный медицинский университет» Минздрава России

Natalia Aleksandrovna Kirillova

Siberian State Medical University

Email: kirillova@mail.ru

Russian Federation кандидат медицинских наук, ассистент кафедры общей врачебной практики и поликлинической терапии ГБОУ ВПО «Сибирский государственный медицинский университет» Минздрава России

Vadim Markovich Govorun

Scientific Research Institute of Physical-Chemical Medicine;
Kazan Federal University

Email: niifhm@fmbamail.ru

Russian Federation доктор биологических наук, профессор, член-корреспондент РАН, заместитель директора по научной работе ФГБУН «Научно-исследовательский институт физико-химической медицины» ФМБА России, главный научный сотрудник научно-исследовательской лаборатории «Омиксные технологии» Института фундаментальной медицины и биологии Казанского (Приволжского) федерального университета

Elena Sergeevna Kostryukova

Scientific Research Institute of Physical-Chemical Medicine;
Kazan Federal University

Email: el-es@yandex.ru

Russian Federation

 

 

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