COMPARATIVE STUDY OF PHARMACOKINETICS OF OFLOXACIN IN A FREE AND NIOSOMAL FORMS IN EXPERIMENTS ON WHITE MICE WHEN ADMINISTERED Per Os

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Abstract


Aim: to study features of pharmacokinetics of ofloxacin as a part of anion PEGylated niosomes on a basis of sorbitan monostearate (Span 60) to experimental white mice per os. Materials and methods: ofloxacin was entrapped in niosomes consisting of Span 60, cholesterol, PEG 4000 and dicetylphosphate. Sizes of niosomes estimated by means of probe microscopy. Efficiency of inclusion of an antibiotic in niosomes defined after removal of free drug by a centrifugation. The analysis of the quantitative contents of ofloxacin in samples carried out a method of a high performance liquid chromatography. Results: we studied the main pharmacokinetic parameters of ofloxacin when used free and niosomal forms of antibiotic to experimental white mice per os. It is shown that use of oral niosomal forms leads to decrease of maximal concentration in serum and increase of ofloxacin half-life by 7,4 times in average compared to the free form. It is determined that bioavailability of ofloxacin in the niosomal form is 154% relative to the free form of the antibiotic. Conclusions: niosomal microcontainers are perspective technology of encapsulation and the directed transport of antibacterial preparations through biological barriers. Using of niosomal formulation of ofloxacin is able to afford to increase considerably efficiency of treatment in comparison with a free form and significantly decrease negative effects of antibiotic therapy.


About the authors

A. N. Kulichenko

The Federal Goverment Health Institution «Stavropol Plague Control Reseach Institute» of the Federal Service for Supervision in the Sphere of Consumer Rights Protection and Human Welfare

Author for correspondence.
Email: snipchi@mail.stv.ru

Russian Federation

доктор медицинских наук, директор ФКУЗ «Ставропольский научно-исследовательский противочумный институт» Роспотребнадзора
Адрес: 355035, Ставрополь, ул. Советская, д. 13-15, тел.: (8652) 26-11-21, 26-03-49

M. E. Mikhailova

The Federal Goverment Health Institution «Stavropol Plague Control Reseach Institute» of the Federal Service for Supervision in the Sphere of Consumer Rights Protection and Human Welfare

Email: snipchi@mail.stv.ru

Russian Federation

младший научный сотрудник лаборатории подготовки специалистов ФКУЗ «Ставропольский научно-исследовательский противочумный институт» Роспотребнадзора
Адрес: 355035, Ставрополь, ул. Советская, д. 13-15, тел.: (8652) 26-11-21, 26-03-49

D. A. Kovalev

The Federal Goverment Health Institution «Stavropol Plague Control Reseach Institute» of the Federal Service for Supervision in the Sphere of Consumer Rights Protection and Human Welfare

Email: alcheem@mail.ru

Russian Federation

кандидат химических наук, руководитель сектора биохимии лаборатории подготовки специалистов ФКУЗ «Ставропольский научно-исследовательский противочумный институт» Роспотребнадзора Адрес: 355035, Ставрополь, ул. Советская, д. 13-15, тел.: (8652) 26-11-21, 26-03-49

S. V. Pisarenko

The Federal Goverment Health Institution «Stavropol Plague Control Reseach Institute» of the Federal Service for Supervision in the Sphere of Consumer Rights Protection and Human Welfare

Email: snipchi@mail.stv.ru

Russian Federation

кандидат химических наук, старший научный сотрудник сектора биохимии лаборатории подготовки специалистов ФКУЗ «Ставропольский научно-исследовательский противочумный институт» Роспотребнадзора
Адрес: 355035, Ставрополь, ул. Советская, д. 13-15, тел.: (8652) 26-11-21, 26-03-49

U. V. Siriza

The Federal Goverment Health Institution «Stavropol Plague Control Reseach Institute» of the Federal Service for Supervision in the Sphere of Consumer Rights Protection and Human Welfare

Email: snipchi@mail.stv.ru

Russian Federation

младший научный сотрудник сектора биохимии лаборатории подготовки специалистов ФКУЗ «Ставропольский научно-исследовательский противочумный институт» Роспотребнадзора
Адрес: 355035, Ставрополь, ул. Советская, д. 13-15, тел.: (8652) 26-11-21, 26-03-49

L. V. Lyapustina

The Federal Goverment Health Institution «Stavropol Plague Control Reseach Institute» of the Federal Service for Supervision in the Sphere of Consumer Rights Protection and Human Welfare

Email: snipchi@mail.stv.ru

Russian Federation

доктор медицинских наук, руководитель лаборатории подготовки специалистов ФКУЗ «Ставропольский научно-исследовательский противочумный институт» Роспотребнадзора
Адрес: 355035, Ставрополь, ул. Советская, д. 13-15, тел.: (8652) 26-11-21, 26-03-49

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