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Two approaches to somatic point mutations in 12 and 13 codones of K-ras gene were analyzed: PCR/SSCP/AСRS/sequencing and allele-specific PCR in the real-life regimen (Russian set «KRAS-7M»). The comparison was carried out on 62 examples of genomic DNA extracted from frozen colon carcinomas, which underwent manual dissection. The results obtained in two attempts were consistent in 95,2% (N=59). Specificity and sensitivity of K-ras mutations detection using «KRAS-7M» set were 100 and 96,4% respectively, and 94,1 and 100% respectievly using PCR/SSCP/AСRS/ automatic sequencing. False positive results were absent when detecting with «KRAS-7M» and accounted for 2 cases (5,9%) when using PCR/SSCP/ AСRS/automatic sequencing. The only false negative response (3,6%) was obtained analyzing mutations using «KRAS-7M».

About the authors

F. A. Amosenko

Scientific centre of medical genetics, RAMS, Moscow
Blokhin Russian oncological scientific centre RAMS, Moscow

Author for correspondence.
кандидат биологических наук, старший научный сотрудник, ведущий научный сотрудник Медико-генетического научного центра РАМН, лаборатории ДНК-диагностики Адрес: 115478, Москва, ул. Москворечье, д. 1 Тел.: (965) 132-78-02, факс: (499) 324-07- 02 Russian Federation

I. V. Karpov

Laboratory of genetic diagnosis «BioLink», Novosibirsk

лаборант ООО «БиоЛинк» лаборатории генодиагностики Адрес: 630117, Новосибирск, ул. Академика Тимакова, д. 2 Тел./факс: (383) 334-86-14 Russian Federation

A. V. Polyakov

Scientific centre of medical genetics, RAMS, Moscow

доктор биологических наук, профессор Медико-генетического научного центра РАМН, заведующий лабораторией ДНК-диагностики Адрес: 115478, Москва, ул. Москворечье, д. 1 Тел./факс: (495) 221-90-84 Russian Federation

S. P. Kovalenko

Institute of molecular biology and biophysics Siberian division of RAMS, Novosibirsk

кандидат биологических наук, заведующий лабораторией генной инженерии Института молекулярной биологии и биофизики Сибирского Отделения РАМН Адрес: 630117, Новосибирск, ул. Академика Тимакова, д. 2 Тел/факс: (383) 333-47-10 Russian Federation

V. A. Shamanin

Laboratory of genetic diagnosis «BioLink», Novosibirsk

кандидат биологических наук, ведущий научный сотрудник лаборатории гено- диагностики ООО «БиоЛинк» Адрес: 630117, Новосибирск, ул. Академика Тимакова, д. 2 Тел/факс: (383) 334-86-14 Russian Federation

L. N. Lyubchenko

Blokhin Russian oncological scientific centre RAMS, Moscow

доктор медицинских наук, заведующая лабораторией клинической онкогенетики Российского онкологического научного центра им. Н.Н. Блохина РАМН Адрес: 115478, Москва, Каширское шоссе, д. 24 Тел.: (916) 623-23-66, факс: (499) 324-96-29 Russian Federation


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