Influence of Collagen Production, Collagenase Activity and Amino Acids Level on Formation of Fetal Growth Restriction in Women with Placental Insufficiency

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Abstract

Rationale. According to WHO, about 21 million children are born annually in the world with low body weight. Currently, the problems of etiology, diagnosis and treatment of fetal growth retardation (FGR) are very relevant for obstetrics and perinatology. Purpose — to study the level of collagen (COL1) collagenase activity (MMP1), and content of proline, ornithine in the maternal blood serum, amniotic fluid, placenta and cord blood of patients with placental insufficiency, and to identify their role in the formation of fetal growth restriction (FGR) during early pregnancy. Methods. 150 women were examined, which made up 2 groups — control (with uncomplicated pregnancy, n = 105) and main (with IUGR, n = 45). All measurements were performed in maternal blood serum, amniotic fluid, placenta and cord blood of women of both groups. Collagen content and collagenase activity are determined by ELISA kits, USA. Determination of amino acids (proline, ornithine) was carried out by capillary electrophoresis on the device “Capel-105” (St. Petersburg). Results. As a result of the correlation analysis in the study of all biofluids by the Spearman method with the calculation of the rank correlation coefficient (r) in the group of women with FGR, significant correlations between collagen production and collagenase activity were revealed. The most pronounced interdependence (r = 0.85; p < 0.05) was found in the maternal blood serum. Conclusions. A relationship was found between the studied parameters (collagen, collagenase, proline, ornithine) in blood serum, amniotic fluid, cord blood and placenta of women with placental insufficiency and their role in the formation of fetal growth retardation was established.

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About the authors

Irina I. Krukier

Rostov State Medical University

Author for correspondence.
Email: biochem@rniiap.ru
ORCID iD: 0000-0003-4570-6405
SPIN-code: 4975-1350

PhD in Biology

Russian Federation, Rostov-on-Don

Valeriya V. Avrutskaya

Rostov State Medical University

Email: v.avrutskaya@rniiap.ru
ORCID iD: 0000-0001-6399-5007
SPIN-code: 9495-9702

MD, PhD, Associate Professor

Russian Federation, Rostov-on-Don

Marina A. Levkovich

Rostov State Medical University

Email: xlma@mail.ru
ORCID iD: 0000-0001-8047-7148
SPIN-code: 2964-0480

MD, PhD, Associate Professor

Russian Federation, Rostov-on-Don

Alina V. Galusyak

Rostov State Medical University

Email: galusyak.alina@list.ru
ORCID iD: 0000-0003-2338-6375

PhD Student

Russian Federation, Rostov-on-Don

Armen A. Grigoriants

Stavropol State Medical University

Email: mr.vanhelsink@mail.ru
ORCID iD: 0000-0002-9333-2578
SPIN-code: 6945-4464

MD, PhD

Russian Federation, Stavropol

Anastasiya A. Nikashina

Rostov State Medical University

Email: laigash@yandex.ru
ORCID iD: 0000-0001-8099-9093
SPIN-code: 6693-3430

PhD in Biology

Russian Federation, Rostov-on-Don

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Supplementary files

Supplementary Files
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1. Figure 1 Study Design

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2. Figure 2. Dynamics of collagen, collagenase activity, proline and ornithine content in blood serum and amniotic fluid in women with IUGR, %

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3. Figure 3. Dynamics of collagen, collagenase activity, proline and ornithine content in cord blood and placenta in women with IGR, %

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