INDICES OF MATRIX METALLOPROTEINASES (MMP-2, MMP-9, TIMP-1) WITH JUVENILE ARTHRITIS IN CHILDREN

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Abstract

One of the perspectives of modern rheumatology is the study of  matrix metalloproteinases (MMP) in juvenile arthritis - enzymes that play a key role in the process of joint destruction. Aim. To analyse the content of matrix MMP-2 and MMP-9 and their tissue inhibitor (TIMP-1) in blood serum and synovial fluid in various embodiments of juvenile arthritis in children. Patients and Methods.The study involved 82 children with juvenile arthritis, and 20 healthy children. The level of MMP-2, MMP-9 and TIMP-1 were determined in serum and synovial fluid by ELISA. Results. It was found out that with any form of arthritis serum concentrations of MMP-2, MMP-9 and TIMP-1 was significantly higher than control values, but the level of MMP-2 in a subset of enthesitis-related arthritis, didn’t differ from the control. Studied parameters in the synovial fluid were much higher than the serum level. With the development of uveitis TIMP-1 in blood serum was lower than in the absence of eye damage. On treatment of patients significant changes in the studied enzymes weren’t established. On a good response to therapy the level of MMP-9 in serum decreased, on the lack of effectiveness – increased. Conclusions. The analysis results  confirm the involvement of MMP-2, MMP-9 and TIMP-1 in the processes of inflammatory changes of the joints in juvenile idiopathic arthritis (JIA) and reactive arthritis, regardless the patients’sex or age.

 

About the authors

A. N. Kailina

Siberian State Medical University, Tomsk, Russian Federation

Author for correspondence.
Email: kaylinan@mail.ru
PhD student, Pediatrics Department of the Medical Faculty with Children's Diseases Course, Siberian State Medical University Address: 634050, Tomsk, Moscow path, 2; tel.: (3822) 90-53-84 Россия

L. M. Ogorodova

Siberian State Medical University, Tomsk, Russian Federation

Email: lm-ogorodova@mail.ru
PhD, Professor, RAMS cor. member, Head of the Pediatrics Department of the Medical Faculty with Children's Diseases Course, Siberian State Medical University Address: 634050, Tomsk, Moscow path, 2; tel.: (3822) 90-53-84 Россия

Yu. P. Chasovskikh

Siberian State Medical University, Tomsk, Russian Federation

Email: juliaclock@rambler.ru
PhD, Assistant, Pediatrics Department of the Medical Faculty with Children's Diseases Course, Siberian State Medical University Address: 634050, Tomsk, Moscow path, 2; tel.: (3822) 90-53-84 Россия

E. E. Kremer

Siberian State Medical University, Tomsk, Russian Federation

Email: elenakremer@yandex.ru
PhD, Junior Research Worker, Central Research Laboratory of Siberian State Medical University Address: 634050, Tomsk, Moscow path, 2; tel.: (3822) 52-83-97 Россия

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