Expression of adipocytokine in heart fat depots depending on the degree of coronary artery atherosclerosis in patients with coronary artery disease

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Abstract

Background. In coronary artery disease, a change in the adipocytokine content of local fat depots of the heart is observed. However, it has not yet been established whether the expression levels of the studied parameters are really related to the degree of atherosclerotic lesion of the spacecraft.

Aims — to identify the features of the expression of adiponectin, leptin and IL-6 by adipocytes of epicardial, perivascular and subcutaneous adipose tissue depending on the degree of atherosclerotic lesion of the coronary channel in coronary heart disease.

Materials and methods. The study conducted at the “Research Institute for Complex Issues of Cardiovascular Disease” in 2017–2020, included 84 patients with coronary heart disease (CAD), of which 39 with a moderate degree of atherosclerotic lesion of the coronary artery (CA) (≤ 22 points on the SYNTAX Score scale), 20 with severe (23–31 points) and 25 with extremely severe (≥ 32 points). Biopsies of subcutaneous (SAT), epicardial (EAT) and perivascular adipose tissue (PVAT) were obtained during elective coronary artery bypass grafting (CABG). The expression of adipocytokine genes was determined using polymerase chain reaction (real-time PCR) using TaqMan probes and the concentration of the studied adipocytokines in adipocyte culture medium by enzyme immunoassay. Statistical analysis was performed using Statistica 9.0, a one-dimensional and multi-dimensional logistic regression analysis.

Results. In CAD in adipocytes of the cardiac fat depot, a shift in the balance of adipocytokines is observed towards increased expression and secretion of leptin, IL-6 and a decrease in adiponectin with maximum manifestation in severe and extremely severe coronary lesions. Adipocytes of EAT were characterized by the minimal expression of the adiponectin gene against the background of the maximum — leptin and IL-6 in comparison with adipocytes SAT and PVAT.

Conclusions. Low expression of the adiponectin in EAT and PVAT against the background of increased expression of leptin and IL-6 is associated with an increase in the degree of atherosclerotic lesion of the coronary channel.

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About the authors

Olga V. Gruzdeva

Research Institute for Complex Issues of Cardiovascular Disease

Email: o_gruzdeva@mail.ru
ORCID iD: 0000-0002-7780-829X
SPIN-code: 4322-0963

MD, PhD

Russian Federation, Kemerovo

Yulia A. Dyleva

Research Institute for Complex Issues of Cardiovascular Disease

Email: dyleva87@yandex.ru
ORCID iD: 0000-0002-6890-3287
SPIN-code: 2064-6262

MD, PhD

Russian Federation, Kemerovo

Ekaterina V. Belik

Research Institute for Complex Issues of Cardiovascular Disease

Author for correspondence.
Email: sionina.ev@mail.ru
ORCID iD: 0000-0003-3996-3325
SPIN-code: 5705-9143

Junior Research Associate

Russian Federation, Kemerovo

Olga E. Akbasheva

Research Institute for Complex Issues of Cardiovascular Disease

Email: akbashoe@yandex.ru
ORCID iD: 0000-0003-0680-8249
SPIN-code: 8042-6940

MD, PhD, Professor

Russian Federation, Kemerovo

Daria A. Borodkinа

Research Institute for Complex Issues of Cardiovascular Disease

Email: alpheia@mail.ru
ORCID iD: 0000-0002-6221-3509
SPIN-code: 8666-3500

MD, PhD, Senior Research Associate

Russian Federation, Kemerovo

Maxim Y. Sinitsky

Research Institute for Complex Issues of Cardiovascular Disease

Email: sinimu@kemcardio.ru
ORCID iD: 0000-0002-4824-2418
SPIN-code: 1095-2352

Research Associate

Russian Federation, Kemerovo

Danil Y. Naumov

Research Institute for Complex Issues of Cardiovascular Disease

Email: naumdu@kemcardio.ru
ORCID iD: 0000-0002-3972-3551
SPIN-code: 1087-1748

Laboratory Assistant

Russian Federation, Kemerovo

Evgeniya E. Bychkova

Research Institute for Complex Issues of Cardiovascular Disease

Email: eugenia.tarasowa@yandex.ru
ORCID iD: 0000-0002-0500-2449
SPIN-code: 8247-9881

Laboratory Assistant

Russian Federation, Kemerovo

Elena Fanaskova

Research Institute for Complex Issues of Cardiovascular Disease

Email: fanaskova70@mail.ru
ORCID iD: 0000-0003-2705-3252
SPIN-code: 8829-7720

MD, PhD, Senior Research Associate

Russian Federation, Kemerovo

Elena I. Palicheva

Research Institute for Complex Issues of Cardiovascular Disease

Email: palichevaelena@rambler.ru
ORCID iD: 0000-0002-5642-7746
SPIN-code: 6437-3222

MD, PhD, Senior Research Associate

Russian Federation, Kemerovo

Anastasia A. Kuzmina

Research Institute for Complex Issues of Cardiovascular Disease

Email: stusha76@mail.ru
ORCID iD: 0000-0002-4807-7686
Scopus Author ID: 788345

MD, PhD, Professor, Corresponding Member of the RAS

Russian Federation, Kemerovo

Olga Barbarash

Research Institute for Complex Issues of Cardiovascular Disease

Email: barb61@yandex.ru
ORCID iD: 0000-0002-4642-3610
SPIN-code: 5373-7620

MD, PhD, Professor, Corresponding Member of the RAS

Russian Federation, Kemerovo

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