Dynamics of serum and placental products of cytokines, oxytocin and relaxin of women with gestational diabetes mellitus

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Background. Pregnancy and diabetes mellitus (GDM) developed during this period are extremely unfavorable combinations for both the mother and the fetus, leading to severe consequences in the gestational period. GDM significantly increases the frequency of undesirable outcomes of pregnancy and childbirth, and also poses a serious medical and social problem for both the mother and the fetus.

Aims — to study the level of cytokines (TGFβ and IL-1β), oxytocin and relaxin in patients with gestational diabetes mellitus (GDM) in the blood serum and placenta and to show their role in the choice of delivery tactics.

Materials and methods. We examined 230 women who made up 2 groups — the control (with the physiological course of pregnancy, n = 95) and the main (with gestational diabetes, n = 135). All studies were performed in the blood serum and placenta of women of these groups. The content of TGFβ and IL-1β was determined by enzyme-linked immunosorbent assay using the R&D system (USA) and Cytimmune systems (USA) kits, and the level of relaxin and oxytocin was measured using BIOSOURCE (USA) kits.

Results. We studied the production of cytokines and relaxants in the blood serum and placenta of women with GDM is in close relationship, which is confirmed by the revealed correlation dependencies: between relaxin before birth and after they have ended (among themselves, r = 0.79; p < 0.05), as well as oxytocin (among themselves, r = 0.78; p < 0.05) in the same period. After the end of labor, a negative relationship was found in the placenta between relaxin and IL-1β (r = –0.61; p < 0.01).

Conclusions. The ratios of relaxin to oxytocin in blood serum revealed by us can be considered as possible prognostic indicators for choosing the tactics of labor management. The use of identified biochemical markers will allow timely selection of the method of delivery in women with GDM in order to reduce the risk of complications in childbirth and thereby favorably influence perinatal outcomes and reduce the incidence of newborns.

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About the authors

Irina I. Krukier

Rostov State Medical University

Author for correspondence.
Email: biochem@rniiap.ru
ORCID iD: 0000-0003-4570-6405
SPIN-code: 4975-1350
Scopus Author ID: 56098706200

Russian Federation, 29, Nachitsevanskij, Rostov-on-Don, 344022

PhD in Biology, Leader Research Associate

Valeriya V. Avrutskaya

Rostov State Medical University

Email: v.avrutskaya@rniiap.ru
ORCID iD: 0000-0001-6399-5007
SPIN-code: 9495-9702

Russian Federation, 29, Nachitsevanskij, Rostov-on-Don, 344022

MD, PhD, Associate Professor, Chief Research Associate

Marina A. Levkovich

Rostov State Medical University

Email: biochem@rniiap.ru
ORCID iD: 0000-0001-8047-7148
SPIN-code: 2964-0480

Russian Federation, 29, Nachitsevanskij, Rostov-on-Don, 344022

MD, PhD, Associate Professor, Leader Research Associate

Armen A. Grigoriants

Stavropol State Medical University

Email: mr.vanhelsink@mail.ru
ORCID iD: 0000-0002-9333-2578
SPIN-code: 6945-4464

Russian Federation, 310, Mira street, Stavropol, 355017


Yuri A. Petrov

Rostov State Medical University

Email: mr.doktorpetrov@mail.ru
ORCID iD: 0000-0002-2348-8809
SPIN-code: 1582-0468

Russian Federation, 29, Nachitsevanskij, Rostov-on-Don, 344022

MD, PhD, Professor

Anastasiya A. Nikashina

Rostov State Medical University

Email: Laigash@yandex.ru
ORCID iD: 0000-0001-8099-9093
SPIN-code: 6693-3430
Scopus Author ID: 36696636000

Russian Federation, 29, Nachitsevanskij, Rostov-on-Don, 344022

PhD in Biology, Research Associate

Alexei L. Chikin

Federal Research Centre the Southern Scientifi c Centre of the Russian Academy of Sciences

Email: chikin@sfedu.ru
ORCID iD: 0000-0002-4065-010X
SPIN-code: 3845-9760

Russian Federation, 41, Chekhova ave., Rostov-on-Don, 344006

PhD in Pharmaceutical Sciences, Chief Research Associate


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Supplementary files

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Figure: 1. Study design

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Figure: 2. Dynamics of cytokines, oxytocin and relaxin in blood serum (taken before and after childbirth) in women with gestational diabetes mellitus,% to control - physiological pregnancy

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Figure: 3. Dynamics of cytokines, oxytocin and relaxin in the placenta of women with gestational diabetes mellitus,% to control - physiological pregnancy

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Рис. 4. Динамика цитокинов, окситоцина и релаксина (в % к контролю - физиологическая беременность) в сыворотке крови (взятой до и после родов) и плаценте женщин с гестационным сахарным диабетом.

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