C-fos, ERK1/2, MAP2, NOTCH1 Proteins Expression Patterns in Human Cerebral Cortex Neurons after Ischemic Stroke

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  • Authors: Sergeeva S.P.1, Lyundup A.V.1, Beregovykh V.V.1, Litvitskiy P.F.1, Savin A.A.2, Gorbacheva L.R.3, Kiseleva E.V.4, Breslavich I.D.3, Kutsenko K.I.5,6, Shishkina L.V.7
  • Affiliations:
    1. I.M. Sechenov First Moscow State Medical University (Sechenov University)
    2. A.I. Yevdokimov Moscow State University of Medicine and Dentistry
    3. Lomonosov Moscow State University
    4. Koltzov Institute of Developmental Biology of Russian Academy of Sciences
    5. Bureau of Forensic Medicine of Moscow Healthcare Department
    6. International Legal Institute
    7. N.N. Burdenko Scientific Research Neurosurgery
  • Issue: Vol 75, No 3 (2020)
  • Pages: 226-233
  • Section: NEUROLOGY AND NEUROSURGERY: CURRENT ISSUES
  • URL: https://vestnikramn.spr-journal.ru/jour/article/view/1295
  • DOI: https://doi.org/10.15690/vramn1295
  • Cite item
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Abstract


Background. The search for protein (these include c-fos, ERK1/2, MAP2, NOTCH1) expression that provide neuroplasticity mechanisms of the cerebral cortex after ischemic stroke (IS) patterns is an urgent task.

Aims — to reveal c-fos, ERK1/2, MAP2, NOTCH1 proteins expression patterns in human cerebral cortex neurons after IS.

Materials and methods. We studied 9 left middle cerebral artery (LMCA) IS patients cerebral cortex samples from 3 zones: 1 — the zone adjacent to the necrotic tissue focus; 2 — zone remote from the previous one by 4–7 cm; 3 — zone of the contralateral hemisphere, symmetric to the IS focus. Control samples were obtained from 3 accident died people. Identification of targeted proteins NSE, c-fos, ERK1/2, MAP2, NOTCH1 was performed by indirect immunoperoxidase immunohistochemical method.

Results. Moving away from the ischemic focus, there is an increase in the density of neurons and a decrease in the damaged neurons proportion, the largest share of c-fos protein positive neurons in zone 2, NOTCH1 positive neurons in zone 1, smaller fractions of ERK1/2 and MAP2 positive neurons compared to the control only in samples of zone 1.

 Conclusions. With the IS development, the contralateral hemisphere is intact tissue increased activation zone, while the zones 1 and 2 have pathological activation signs. In zone 1 of the range, the adaptive response of the tissue decreases, and in zone 2 it expands. Therefore, a key target for therapeutic intervention is zone 2.


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About the authors

Svetlana P. Sergeeva

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Author for correspondence.
Email: svetlanapalna@mail.ru
ORCID iD: 0000-0002-0083-1213
SPIN-code: 4257-9498

Russian Federation, 8, bld. 2, Trubetskaya str., 119991, Moscow

MD, PhD

Aleksey V. Lyundup

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Email: lyundup@gmail.com
ORCID iD: 0000-0002-0102-5491
SPIN-code: 4954-3004

Russian Federation, Moscow

MD, PhD

Valery V. Beregovykh

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Email: beregovykh@ramn.ru
ORCID iD: 0000-0002-0210-4570
SPIN-code: 5940-7554

Russian Federation, Moscow

MD, PhD, Professor, Aсademiсian of the RAS

Petr F. Litvitskiy

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Email: litvicki@mma.ru
ORCID iD: 0000-0003-0151-9114
SPIN-code: 6657-5937

Russian Federation, Moscow

MD, PhD, Professor, Corresponding Member of the RAS

Aleksey A. Savin

A.I. Yevdokimov Moscow State University of Medicine and Dentistry

Email: lasavin@mail.ru
ORCID iD: 0000-0002-6340-8623
SPIN-code: 9162-6720

Russian Federation, Moscow

MD, PhD, Professor

Liubov R. Gorbacheva

Lomonosov Moscow State University

Email: gorbacheva@mail.bio.msu.ru
ORCID iD: 0000-0002-3910-8831
SPIN-code: 4394-0575

Russian Federation, Moscow

PhD, Professor

Ekaterina V. Kiseleva

Koltzov Institute of Developmental Biology of Russian Academy of Sciences

Email: evkiseleva@mail.ru
ORCID iD: 0000-0002-2270-6595
SPIN-code: 7421-8496

Russian Federation, Moscow

PhD

Ilya D. Breslavich

Lomonosov Moscow State University

Email: br_i@inbox.ru
ORCID iD: 0000-0002-9321-9102
SPIN-code: 9615-9237

Russian Federation, Moscow

ассистент

Kirill I. Kutsenko

Bureau of Forensic Medicine of Moscow Healthcare Department; International Legal Institute

Email: ceamler@gmail.com
ORCID iD: 0000-0001-9299-5153
SPIN-code: 8964-3717

Russian Federation, Moscow

MD, PhD

Lyudmila V. Shishkina

N.N. Burdenko Scientific Research Neurosurgery

Email: lshishkina@nsi.ru
ORCID iD: 0000-0001-7045-7223
SPIN-code: 6341-2050

Russian Federation, Moscow

MD, PhD

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