Micro-RNAs in the Diagnosis of Cutaneous T-Cell Lymphomas

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Abstract

Rationale. Early diagnosis of mycosis fungoides (MF), as the most common form of T-cell lymphoma, presents significant challenges. The diagnosis of MF is based on the following criteria: comprehensive assessment of the clinical picture of the disease, histological and immunohistochemical examination of the skin, and determination of rearrangement of the T-cell receptor gene, but even this does not always aid in diagnosis. The aim of the study is to investigate the expression of micro-RNAs (miR-223, -423, -663, -16, -326, -711) in the blood plasma and leukocytes of patients with a presumptive diagnosis of MF to improve the disease diagnosis. Methods. This study included 50 patients aged 24 to 79 years, of whom 30 patients had a preliminary diagnosis of MF and 20 patients with small plaque parapsoriasis, who formed the comparison group. All patients underwent histological, immunohistochemical examination of skin biopsies, and determination of micro-RNA (miR-223, -423, -663, -16, -326, -711) expression in blood plasma and leukocytes by real-time PCR. Results. Analyzing the results of clinical-anamnestic, histological, and immunohistochemical research methods, the diagnosis of MF was established in 22 of 30 (73.3%) patients, of which 9 of 14 (64.3%) were in the early stages of the disease. small plaque parapsoriasis. Conclusion. During our study, it was found that the studied micro-RNAs (miR-326, -663, -711, -223, -423, -16) in the blood plasma and leukocytes of patients with MF have statistically significant levels of expression compared to the low level of expression of these micro-RNAs in patients with small plaque parapsoriasis. The expression of micro-RNAs we studied in the skin contributes to the improvement of MF diagnosis with an accuracy of up to 90%.

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About the authors

Olga Yu. Olisova

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Author for correspondence.
Email: olisovaolga@mail.ru
ORCID iD: 0000-0003-2482-1754

MD, PhD, Professor, Corresponding Member of the RAS

Россия, Moscow

Jessika R. Amshinskaya

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Email: dr.jessika@yandex.ru
ORCID iD: 0000-0002-3907-2189

PhD Student

Россия, Moscow

Vladimir V. Demkin

Institute of Molecular Genetics of the National Research Center “Kurchatov Institute”

Email: vdemkin@img.ras.ru
ORCID iD: 0000-0002-3408-6100

MD, PhD

Россия, Moscow

References

  1. Воронцова А.А., Карамова А.Э., Знаменская Л.Ф. Современные представления о патогенезе грибовидного микоза // Онкогематология. — 2018. — Т. 13. — № 3. — С. 39–46. [Vorontsova АА, Karamova EА, Znamenskaya LF. Modern concepts of the mycosis fungoides pathogenesis. Onkogematologiya = Oncohematology. 2018;13(3):39–46. (In Russ.)] doi: https://doi.org/10.17650/1818-8346-2018-13-3-39-46
  2. Hodak E, Amitay-Laish I. Mycosis fungoides: a great imitator. Clin Dermatol. 2019;37(3):255–267. doi: https://doi.org/10.1016/j.clindermatol.2019.01.004
  3. Жуков А.С., Белоусова И.Э., Самцов А.В. Иммунологические и молекулярно-генетические механизмы развития грибовидного микоза // Вестник дерматологии и венерологии. — 2015. — Т. 91. — № 4. — С. 42–50. [Zhukov AS, Belousova IE, Samtsov AV. Immunological and molecular genetic mechanisms of the development of mycosis fungoides. Vestnik Dermatologii i Venerologii. 2015;91(4):42–50. (In Russ.)]
  4. Quinodoz S, Guttman M. Long noncoding RNAs: an emerging link between gene regulation and nuclear organization. Trends Cell Biol. 2014;24(11):651–663. doi: https://doi.org/10.1016/j.tcb.2014.08.009
  5. Campbell JJ, Clark R, Watanabe R, et al. Sezary syndrome andmycosis fungoides arise from distinct T-cell subsets: a biologic rationale for their distinct clinical behaviors. Blood. 2010;116(5):767–771. doi: https://doi.org/10.1182/blood-2009-11-251926
  6. Girardi M, Edelson RL. Cutaneous T-cell lymphoma: pathogenesis and treatment. Oncology (Williston Park). 2000;14(7):1061–1070.
  7. Федеральные клинические рекомендации. Дерматовенерология 2015: Болезни кожи. Инфекции, передаваемые половым путем. — 5-е изд., перераб. и доп. — М.: Деловой экспресс, 2016. — 768 с. [Dermatovenerologiya: federal’nye klinicheskie rekomendacii. 2015: Bolezni kozhi. Infekcii, peredavaemye polovym putem. 5-e izd., pererab. i dop. Moscow: Delovoj ekspress; 2016.768 р. (In Russ.)]
  8. Виноградова Ю.Е., Зингерман Б.В. Нозологические формы и выживаемость пациентов с Т- и НК-клеточными лимфатическими опухолями, наблюдающихся в ГНЦ в течение 10 лет // Клиническая онкогематология. — 2011. — T. 4. — № 3. — C. 201–212. [Vinogradova YuE, Zingerman BV. Nosological forms and survival of patients with T- and NK-cell lymphoid neoplasms observed in HSC during 10 years. Clinical Oncohematology. 2011;4(3):201–212. (In Russ.)]
  9. Jawed SI, Myskowski PL, Horwitz S, et al. Primary cutaneous T-cell lymphoma (mycosis fungoides and Sézary syndrome): part I. Diagnosis: clinical and histopatho logic features and new molecular and bio logic markers. J Am Acad Dermatol. 2014;70(2):205.е1–16. doi: https://doi.org/10.1016/j.jaad.2013.07.049
  10. Moyal L, Feldbaum N, Goldfeiz N, et al. The Therapeutic Potential of AN-7, a Novel Histone Deacetylase Inhibitor, for Treatment of Mycosis Fungoides/Sezary Syndrome Alone or with Doxorubicin. PLoS One. 2016:11(1):e0146115. doi: https://doi.org/10.1371/journal.pone.01461151
  11. Benjamin Chase A, Markel K, Tawa MC. Optimizing care and compliance for the treatment of mycosis fungoides cutaneous T-cell lymphoma with mechlorethamine gel. Clin J Oncol Nurs. 2015;19(6):Е131–139. doi: https://doi.org/10.1188/15.CJON.E131-E139
  12. Olisova OY, Grekova EV, Zaletaev DV, et al. Overexpression of STAT4 at early stages of mycosis fungoides: Coincidence or not? Australas J Dermatol. 2021;62(1):e119–e120. doi: https://doi.org/10.1111/ajd.13420
  13. Shen X, Wang B, Li K, et al. MicroRNA Signatures in Diagnosis and Prognosis of Cutaneous T-Cell Lymphoma. J Invest Dermatol. 2018;138(9):2024–2032. doi: https://doi.org/10.1016/j.jid.2018.03.1500
  14. Kozomara A, Griffiths-Jones S. miRBase: integrating microRNA annotation and deep-sequencing data. Nucleic Acids Res. 2018;39(Database issue):D152–157. doi: https://doi.org/10.1093/nar/gkq1027
  15. Zackheim HS, McCalmont TH. Mycosis fungoides: the great imitator. J Am Acad Dermatol. 2002;47(6):914–918. doi: https://doi.org/10.1067/mjd.2002.124696
  16. Peterson SM, Thompson JA, Ufkin ML, et al. Common features of microRNA target prediction tools. Front Genet. 2014;5:23. doi: https://doi.org/10.3389/fgene.2014.00023
  17. Sadakierska-Chudy A. MicroRNAs: Diverse Mechanisms of Action and Their Potential Applications as Cancer Epi-Therapeutics. Biomolecules. 2020;10(9):1285. doi: https://doi.org/10.3390/biom10091285
  18. Wong HK, Mishra A, Hake T, et al. Evolving insights in the pathogenesis and therapy of cutaneous T-cell lymphoma (mycosis fungoides and Sezary syndrome). Br J Haematol. 2011;155(2):150–166. doi: https://doi.org/10.1111/j.1365-2141.2011.08852.x
  19. Mardani R, Jafari Najaf Abadi MH, Motieian M, et al. Micro RNA in leukemia: Tumor suppressors and oncogenes with prognostic potential. J Cell Physiol. 2019;234(6):8465–8486. doi: https://doi.org/10.1002/jcp.27776
  20. Chen HN, Liu CM, Yang H, et al. 5-Aminolevulinic acid induces apoptosis via NF-κB/JNK pathway in human oral cancer Ca9-22 cells. J Oral Pathol Med. 2011;40(6):483–490. doi: https://doi.org/10.1111/j.1600-0714.2010.00973.x
  21. Ralfkiaer U, Hagedorn PH, Bangsgaard N, et al. Diagnostic microRNA profiling in cutaneous T-cell lymphoma (CTCL). Blood. 2011;118(22):5891–5900. doi: https://doi.org/10.1182/blood-2011-06-358382
  22. Olivo M, Ali-Seyed M. Apoptosis-signalling mechnisms in human cancer cells induced by Calphostin-PDT. Int J Oncol. 2007;30(3):537–548.
  23. Phillips DC, Woollard KJ, Griffiths HR. The anti-inflammatory actions of methotrexate are critically dependent upon the production of reactive oxygen species. Br J Pharmacol. 2003;138(3):501–511. doi: https://doi.org/10.1038/sj.bjp.0705054
  24. DeSimone JA, Guenova E, Carter JB, et al. Low-dose high-dose-rate brachytherapy in the treatment of facial lesions of cutaneous T-cell lymphoma. J Am Acad Dermatol. 2013;69(1):61–65. doi: https://doi.org/10.1016/j.jaad.2012.12.975
  25. Garibyan L, Cotter SE, Hansen JL, et al. Palliative treatment for in-transit cutaneous metastases of Merkel cell carcinoma using surface/mold computer-optimized high-dose-rate brachytherapy. Cancer J. 2013;19(4):283–287. doi: https://doi.org/10.1097/PPO.0b013e31829e3566
  26. Статистический анализ таблиц 2×2 в диагностических исследованиях / авт.-сост. А.В. Тишков и др. — СПб.: СПбГМУ, 2013. — 17 с. [Statistical Analysis of 2×2 Tables in Diagnostic Studies. Author-comp. A.V. Tishkov et al. St. Petersburg: SPbSMU; 2013. 17 p. (In Russ.)]

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2. Рис. 1. ROC-кривая, соответствующая взаимосвязи miR-223 и грибовидного микоза

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3. Рис. 2. ROC-кривая, соответствующая взаимосвязи miR-16 и грибовидного микоза

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4. Рис. 3. ROC-кривая, соответствующая взаимосвязи miR-711 и грибовидного микоза

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