NEW APPROACHES IN THE REGULATION OF BLOOD PHAGOCYTES AND REDUCTION IN THE FORMATION OF OXYGEN RADICALS IN PATIENTS WITH HEART FAILURE

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Abstract

Aim: The purpose was to study the effect of actovegin on the formation of reactive oxygen species by blood phagocytes of patients with heart failure
and on SK-N-SH neuron necrosis. Materials and methods: The generation of superoxide anion (O2-•) were recorded on whole blood samples (50–100 μl). Change lucigenin-dependent hemiluminescence determined on a hemi-luminometer «Biotoks-7». As a stimulator of the phagocyte phorbol ester (PMA, 1 μm) was used. Necrosis of neurons induced by hydrogen peroxide was determined by fluorescence of propidium iodit. Results: Blood phagocytes of heart failure patients are initially pre-activated (primed). These cells spontaneous generated oxygen radicals. Actovegin dosadependent decreased radicals level and radical induced by PMA (1 μm). After PMA maximal inhibitory effect of actovegin observed in doses higher than 2–3 mg/ml. The impact of actovegin on the viability of human SK-N-SH neurons in the presence hydrogen peroxide (100 μm) was studied in
vitro. Under these conditions hydrogen peroxide triggered radical-dependent neurons necrosis Actovegin dosa-dependent decreased of neuron death.
Conclusion: Actovegin inhibits spontaneous and induced formation of reactive oxygen species generated by blood phagocytes of patients with heart failure. Actovegin suppressed necrosis of human SK-N-SH neuroblastoma cells caused by hydrogen peroxide. It is assumed that actovegin protects cells of various organs and tissues, including blood cells and neurons that die as a result of ischemia and inflammation by reducing levels of reactive oxygen species.

About the authors

E. I. Astashkin

I.M. Sechenov First Moscow State Medical University

Author for correspondence.
Email: 287ast@mail.ru

PhD, professor of Pathological Department, Head of the Laboratory of extreme states of Research Centre of  I.M. Sechenov First MSMU

 

Russian Federation

M. G. Glezer

I.M. Sechenov First Moscow State Medical University, Moscow, Russian Federation

Email: 287ast@mail.ru
доктор медицинских наук, профессор кафедры профилактической и неотложной кардио- логии, заведующая лабораторией функциональных методов исследования и рациональной фармакотерапии сер- дечно-сосудистых заболеваний НИЦ Первого МГМУ им. И.М. Сеченова Адрес: 119992, Москва, ул. Трубецкая д. 8, стр. 1, тел.: +7 (499) 972-96-12 Russian Federation

M. G. Vinokurov

I.M. Sechenov First Moscow State Medical University, Moscow, Russian Federation

Email: 287ast@mail.ru
доктор биологических наук, главный научный сотрудник лаборатории экстре- мальных состояний НИЦ Первого МГМУ им. И.М. Сеченова Адрес: 119992, Москва, ул. Трубецкая д. 8, стр. 1, тел.: +7 (495) 622-96-01 Russian Federation

N. S. Orekhova

I.M. Sechenov First Moscow State Medical University, Moscow, Russian Federation

Email: 287ast@mail.ru
кандидат медицинских наук, старший научный сотрудник лаборатории экстре- мальных состояний НИЦ Первого МГМУ им. И.М. Сеченова Адрес: 119992, Москва, ул. Трубецкая д. 8, стр. 1, тел.: +7 (495) 622-96-01 Russian Federation

N. D. Egorova

I.M. Sechenov First Moscow State Medical University, Moscow, Russian Federation

Email: 287ast@mail.ru
научный сотрудник лаборатории экстремальных состояний НИЦ Первого МГМУ им. И.М. Сеченова Адрес: 119992, Москва, ул. Трубецкая д. 8, стр. 1, тел.: +7 (495) 622-96-01 Russian Federation

A. N. Novikova

I.M. Sechenov First Moscow State Medical University, Moscow, Russian Federation

Email: 287ast@mail.ru
лаборант-исследователь лаборатории экстремальных состояний НИЦ Первого МГМУ им. И.М. Сеченова Адрес: 119992, Москва, ул. Трубецкая д. 8, стр. 1, тел.: +7 (495) 622-96-01 Russian Federation

M. M. Yurinskaya

I.M. Sechenov First Moscow State Medical University, Moscow, Russian Federation

Email: 287ast@mail.ru
кандидат биологических наук, старший научный сотрудник лаборатории экстре- мальных состояний НИЦ Первого МГМУ им. И.М. Сеченова Адрес: 119992, Москва, ул. Трубецкая д. 8, стр. 1, тел.: +7 (495) 622-96-01 Russian Federation

S. V. Grachev

I.M. Sechenov First Moscow State Medical University, Moscow, Russian Federation

Email: grachevscience@gmail.com
доктор медицинских наук, академик РАН, заведующий кафедрой патологии Первого МГМУ им. И.М. Сеченова Адрес: 119992, Москва, ул. Трубецкая д. 8, стр. 1, тел.: +7 (499) 248-31-22 Russian Federation

K. E. Sobolev

I.M. Sechenov First Moscow State Medical University, Moscow, Russian Federation

Email: 287ast@mail.ru
рациональной фармакотерапии сердечно-сосудистых заболеваний НИЦ Первого МГМУ им. И.М. Сеченова Адрес: 119992, Москва, ул. Трубецкая д. 8, стр. 1, тел.: +7 (499) 972-96-12 Russian Federation

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