AGE-RELATED CHANGES OF THE PITUITARY FOLLICULO-STELLATE CELLS IN RATS IN CHRONIC STRESS

Cover Page


Cite item

Full Text

Abstract

Folliculo-stellate cells are known as S-100 protein immunopositive cells of the anterior lobe of pituitary gland which are not secreting hormones and are presumed to be organ specific stem cells of the adenohypophys. Their role in adaptation of the body to stress remains unclear. Aim: to evaluate dynamics of folliculo-stellate and hormone-producing cells in rats of different age in chronic stress exposure. Materials and methods: Sprague-Dawley rats aged 1, 3, 6 and 12 months were exposed to chronic immersion immobilization stress. Histological section of the pituitary glands were stained immunohistochemically with subsequent image analysis. Results: in control rats S-100 protein-immunopositive cells increased in number with age and negatively correlated with the number of ACTH-positive cells. In experimental animals aged 1 and 3 months volume density of S-100 protein-immunoreactive cells significantly decreased and negatively correlated with ACTH-positive cells, while in 6 month old rats it was only slightly decreased and in 12 months old animals showed tendency to increase. Conclusions: results of the study demonstrating capacity of the folliculo-stellate cells to contribute to the plasticity of the hypothalamo-hypophyseo-adrenal axis in chronic stress exposure at different age.

 

About the authors

M. Yu. Kapitonova

UiTM, Selangor, Malaysia

Author for correspondence.
Email: marina@salam.uitm.edu.my
PhD, professor, Head of the Anatomy Department of General Medicine Faculty of University Technology MARA. Address: Shah Alam, Selangor, 40150 Malaysia; tel.: (6017) 624-36-99 Malaysia

M. Ullah

UiTM, Selangor, Malaysia

Email: muzammil@salam.uitm.edu.my
PhD, professor of the Anatomy Department of Dentistry Faculty of University Technology MARA. Address: Shah Alam, Selangor, 40150 Malaysia; tel.: (6012) 963-80-75 Malaysia

S. L. Kuznetsov

First Moscow State Medical University n.a. I.M. Sechenov, Russian Federation

Email: vakmedbiol@rambler.ru
PhD, professor, correspondent member of RAMS, Head of the Department of Histology and Fetology of I.M. Sechenov First MSMU. Address: build. 3, 11, Mokhovaya Street, Moscow, RF, 103009; tel.: +7 (495) 698-56-31 Russian Federation

V. V. Khlebnikov

Volgograd State Medical University, Russian Federation

Email: ggnuxa@yandex.ru
assistant of the Neurologic Department of Volgograd State Medical University. Address: 1, Pavshikh bortsov Square, Volgograd, RF, 400066 Russian Federation

M.N.K. Nor-Ashikin

UiTM, Selangor, Malaysia

Email: noras011@salam.uitm.edu.my
PhD, assistant professor, Director of the Institute of molecular health-related biotechnologies of University Technology MARA. Address: Sg Buloh, Selangor, 47000 Malaysia; tel.: (6012) 320-03-48 Malaysia

A. Ahmad

UiTM, Selangor, Malaysia

Email: biotech.azhar@gmail.com
postgraduate of the Anatomy Department of General Medicine Faculty of University Technology MARA. Address: Shah Alam, Selangor, 40150 Malaysia; tel.: (6013) 246-11-06 Malaysia

References

  1. Horiguchi K., Syaidah R., Fujiwara K. et. al. Expression of the cell-surface heparan sulfate proteoglycan syndecan-2 in developing rat anterior pituitary gland. Cell Tissue Res. 2013; 353 (3): 473–481.
  2. Tando Y., Fujiwara K., Yashiro T., Kikuchi M. Localization of Notch signaling molecules and their effect on cellular proliferation in adult rat pituitary. Cell Tissue Res. 2013; 351 (3): 511–519.
  3. Rinehart J.F.,Farquhar M.G. Electron microscopic studies of the anterior pituitary gland. J. Histochem. Cytochem. 1953; 1: 93–113.
  4. Allaerts W., Vankelecom H. History and perspectives of pituitary folliculo-stellate cell research. Eur. J. Endocrinol. 2005; 153: 1–12.
  5. Soji T., Mabuchi Y., Kurono C., Herbert D.C. Folliculo-stellate cells and intercellular communication within the rat anterior pituitary gland. Microsc. Res. Tech. 1997; 39 (2): 138–149.
  6. Inoue K., Mogi C., Ogawa S. et al. Are folliculo-stellate cells in the anterior pituitary gland supportive cells or organ-specific stem cells? Arch. Physiol. Biochem. 2002; 110 (1–2): 50–53.
  7. Itakura E., Odaira K., Yokoyama K. et al. Generation of transgenic rats expressing green fluorescent protein in S-100beta-producing pituitary folliculo-stellate cells and brain astrocytes. Endocrinology. 2007; 148: 1518–1523.
  8. Devnath S., Inoue K. An insight to pituitary folliculo-stellate cells. J. Endocrinol. 2008; 20 (6): 687–691.
  9. Chauvet N., El-Yandouzi T., Mathieu M.N. et al. Characterization of adherens junction protein expression and localization in pituitary cell networks. J. Endocrinol. 2009; 202 (3): 375–387.
  10. Horiguchi K., Ilmiawati C., Fujiwara K. et al. Expression of chemokine CXCL12 and its receptor CXCR4 in folliculostellate (FS) cells of the rat anterior pituitary gland: the CXCL12/CXCR4 axis induces interconnection of FS cells. Endocrinology. 2012; 153 (4): 1717–1724.
  11. Acosta M., Mohamed F. Immunohistochemical and morphometric study of pituitary pars distalis folliculostellate cells of nonpregnant and pregnant viscachas. Biotech. Histochem. 2013; 88 (3–4): 161–169.
  12. Osuna M., Sonobe Y., Itakura E. et al. Differentiation capacity of native pituitary folliculostellate cells and brain astrocytes. J. Endocrinol. 2012; 213 (3): 231–237.
  13. Takagi K., Kasuya Y., Watanabe K. Studies on the drugs from peptic ulcer. A reliable method for producing stress ulcer in rats. Chem. Pharm. Bull (Tokyo.) 1964; 12: 465–472.
  14. Luziga C., Usui M., Yoichiro H., Kazwala R., Yamamoto Y., Mamba K. Gene expression and immunohistochemical localization of megalin in the anterior pituitary gland of helmeted guinea fowl (Numida meleagris). J. Mol. Histol. 2007; 38 (1): 65–77.
  15. Kapitonova M.Y., Kuznetsov S.L., Khlebnikov V.V. et al. Immunohistochemical characteristics of the hypophysis in normal conditions and chronic stress. Neurosci. Behav. Physiol. 2010; 40 (1): 97–102.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 1970 "Paediatrician" Publishers LLC



This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies