EXPRESSION OF APOPTOSIS MARKERS IN ADHESIONS IN THE ABDOMINAL CAVITY UNDER THE EXPERIMENTAL CONDITIONS

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Abstract

Background: Our aim was to study the expression of markers of caspase-dependent and caspase-independent apoptosis pathway activation in the process of repair in case of damage of the serous membrane of the abdominal cavity. Materials and methods: On the experimental model of adhesions in the abdominal cavity (male rats Wistar, n =40) in dynamics from 2 hours to 30 days after injury to the peritoneum studied marker expression proapoptosis (Bcl-x) and anti-apoptosis (Bcl-2), and PARP-1. Results: It was found that in the conditions of traumatic injury of the peritoneum apoptosis and anti-apoptosis occur in parallel. In the initial period of the anti-apoptosis mechanisms prevail, and in later periods dominated phenomena proapoptosis. PARP-1 activation indicates an increase in the frequency of DNA damage cells, and the duration of this process stimulates cell death by caspase-independent pathway. Together, these processes result in the elimination of a large number of cells, especially fibroblast of zone connective tissue formation in an aseptic area of inflammation in the peritoneal injury. We first established that in the case of peritoneal injury marker expression of anti-apoptosis in the damage zone has the character of the two-wave with maximum expression at 1-third day of the pathological process with repetitive peak on the 14th day. Сonclusion: Identifying key parts of apoptosis in the formation of adhesions in the abdominal cavity, which can be used for the development of drugs for the prevention of adhesions.

About the authors

I. A. Shurygina

Scientific Center of Reconstructive and Restorative Surgery, Irkutsk
Irkutsk Scientific Centre

Author for correspondence.
Email: irinashurygina@gmail.com
PhD, Deputy Director for Research and Innovative Activity of Research Center of Reconstructive and anaplastic surgery, chief research scientist of the Department of Medicobiological Study and Technologies of the Irkutsk Scientific Center Россия

M. G. Shurygin

Scientific Center of Reconstructive and Restorative Surgery, Irkutsk, Russian Federation
Irkutsk Scientific Centre, Russian Federation

Email: irinashurygina@gmail.com
PhD, Head of Research and Experimental Department of Research Center of Reconstructive and anaplastic surgery, chief research scientist of the Department of Medicobiological Study and Technologies of the Irkutsk Scientific Center. Address: 1, Bortsov Revolyutsii Street, Irkutsk, RF, 664003; tel.: +7 (3952) 29-03-38 Россия

N. I. Ayushinova

Scientific Center of Reconstructive and Restorative Surgery, Irkutsk, Russian Federation

Email: katnatlove@mail.ru
MD, doctor of the Department of Purulent surgery of Clinic Hospital № 1 of Research Center of Reconstructive and anaplastic surgery. Address: 100, Yubileinyi microraion, Irkutsk, RF, 664079; tel.: +7 (3952) 40-78-74 Россия

References

  1. Karp G. Cell and molecular biology. Concepts and experiments. 6th edn. New York, John Wiley & Sons, 2010. 765 p.
  2. Gordeeva A.V., Labas Y.A., Zvyagilskaya R.A. Apoptosis in unicellular organisms: Mechanisms and evolution. Biochemistry (Moscow). 2004; 69 (10): 1055–1066.
  3. Chao D.T., Korsmeyer S.J. BCL-2 family: regulators of cell death. Ann. Rev. Immunol. 1998; 16: 395–419.
  4. Yu S.-W., Andrabi S.A., Wang H., Kim N.S., Poirier G.G.,
  5. Dawson T.M., Dawson V.L. Apoptosis-inducing factor mediates poly(ADP-ribose) (PAR) polymer-induced cell death. Proceed. Nat. Acad. Sci. 2006; 103: 18314–18319.
  6. Ayushinova N.I., Lepekhova S.A., Shurygina I.A., Roi T.A., Shurygin M.G., Zaritskaya L.V., Gol'dberg O.A. Sposob modelirovaniya spaechnogo protsessa v bryushnoi polosti: Patent RF № 2467401. Zayavka: 2011131678/14, 27.07.2011 [Modeling Method of Adhesions in the Abdominal Cavity: RF Patent # 2,467,401. Application: 2011131678/14, 27.07.2011. Publ. 20.11.2012] 2012. 9 p.
  7. Ayushinova N.I., Shurygina I.A., Shurygin M.G., Lepekhova S.A., Balykina A.V., Malgataeva E.R,, Popova A.D.,Yankelevich S.A. Sib. med. zhurn. = Siberian medical journal. 2012; 109(2): 51–53.
  8. Wight T.N., Potter-Perigo S. The extracellular matrix: an active or passive player in fibrosis? Am. J. Physiol. Gastrointest. Liver Physiol. 2011; 301: 950–955.
  9. Binnebosel M., Klinge U., Rosch R., Junge K., Lynen-Jansen P., Schumpelick V. Morphology, quality, and composition in mature human peritoneal adhesions. Langenbecks Arch. Surg. 2008; 393 (1): 59–66.
  10. Saed G.M., Jiang Z., Fletcher N.M., Diamond MP. Modulation of the BCL-2/BAX ratio by interferon-gamma and hypoxia in human peritoneal and adhesion fibroblasts. Fertil. Steril. 2008; 90 (5):1925–1930.
  11. Saed G.M., Diamond M.P. Molecular characterization of postoperative adhesions: the adhesion phenotype. J. Am. Assoc. Gynecol. Laparosc. 2004; 11 (3): 307–314.

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