CHANGES IN FUNCTIONAL ACTIVITY OF NEUTROPHILS IN PERIPHERAL BLOOD OF PATIENTS WITH RENAL CELL CANCER IN THE COURSE OF THE DISEASE

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Abstract

Objective: Research purpose was to study phagocytic activity and a oxygen depended metabolism of blood neutrophils (NF) at patients with a renal cell carcinoma in dynamics — before operation and after surgical treatment. Methods: Patients with a locally invasive renal cell carcinoma (T3N0M0) were put under observation before surgical treatment (n =84) and in 10 days after a radical nephrectomy (n =54) at the age of 45–55 years. The 56 conditionally healthy donors combine into the control group. Indicators of functional activity, the spontaneous and induced lyuminol- and lyutsigenindependent chemiluminescence (CHL) NF of peripheral blood was studied. Results: During the period before operation time of an exit to a maximum of spontaneous and stimulated lyuminol-dependent HL is slowed down (p <0.004 and p <0.006 respectively); indicators of the area of lyutsigenin-dependent HL NF of blood concerning indicators of control group are increased (p <0.001 for spontaneous, p <0.004 for the reaction induced by lyutsigenin). In 10 days after surgical treatment the quantity of active ingestion rate lowered (p <0.017 in the test with S. epidermidis, p <0.036 for test with latex); time of an exit to a maximum of spontaneous and stimulated lyuminol-dependent remains increased (p <0.006 and p <0.008 respectively) in comparison with indicators of healthy donors. The amount of an index of activation of lyuminol-dependent as in comparison with control (p <0.037), and relative to the period before operation increases (p <0.048). The area of spontaneous lyutsigenin-dependent HL NF of blood relative to indicators of control group is still increased (p <0.011), decrease in this parameter in comparison with amount of group before operation is however registered (p <0.004). Conclusion: At patients with renal cell carcinoma in 10 days after surgical treatment of NF of blood are characterized by the strengthened production of high-energy oxidizers and the reduced phagocytic activity that testifies to a functional and metabolic imbalance.

 

About the authors

L. M. Kurtasova

V.F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Russian Federation

Author for correspondence.
Email: sibmed-obozrenie@yandex.ru
доктор медицинских наук, профессор кафедры клинической иммунологии КрасГМУ им. В.Ф. Войно-Ясенецкого Адрес: 660022, Красноярск, ул. Партизана Железняка, д. 1, тел.: +7 (391) 222-06-28 Россия

E. A. Shkapova

A.I. Kryzhanivskiy Krasnoyarsk Regional Clinical Oncological Centre, Russian Federation

Email: k_shkapova@mail.ru
кандидат биологических наук, биолог клинико-диагностической лаборатории КККОД им. А.И. Крыжановского Адрес: 660133, Красноярск, ул. Смоленская, д. 16, тел.: +7 (391) 267-17-15 Россия

R. A. Zukov

V.F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Russian Federation

Email: zukov.ra@krasgmu.ru
кандидат медицинских наук, доцент кафедры онкологии и лучевой диагностики с курсом ПО КрасГМУ им. В.Ф. Войно-Ясенецкого Адрес: 660022, Красноярск, ул. Партизана Железняка, д. 1, тел.: +7 (391) 251-93-16 Россия

References

  1. Пинегин Б.В., Маянский А.Н. Нейтрофилы: структура и функции. Иммунология. 2007; 28 (6): 374–382.
  2. Shen L., Smith J.M., Shen Z. Differential regulation of neutrophil chemotaxis to IL-8 and fMLP by GM-CSF: lack of direct effect of oestradiol. Immunology. 2006; 117: 205–212.
  3. Нестерова И.В., Ковалева С.В., Чудилова Г.А., Ломтатидзе Л.В., Евглевский А.А. Двойственная роль нейтрофильных гранулоцитов в реализации противоопухолевой защиты. Иммунология. 2012; 33 (5): 281–287.
  4. Мальцева В.Н., Сафронова В.Г. Неоднозначность роли нейтрофилов в генезе опухоли. Цитология. 2009; 51 (6): 467–474.
  5. Fridlender Z.C., Sum J., Kim S., Kapoor V., Cheng G., Ling L., Worthen G. S., Albelda S.M. Polarization of tumor-associated neutrophil phenotype by T6F-b: «N1» versus «N2» TAN. Cancer Cell. 2009; 16 (3): 183–194.
  6. De Sole P., Lippa S., Lixxarru G. Whole blood chеmiluminescence: a new technical approach to access oxygen-dependent microbial activity of granulocytes. J. Clin. Lab. Autom. 1983; 3: 391–400.
  7. Буравкова Л.Б., Ударцева О.О., Андреева Е.Р., Рылова Ю.В. Влияние фотодинамически индуцированной генерации АФК на состояние митохондрий и лизосом культивируемых мезенхимальных стромальных клеток человека. Гены и клетки. 2010; 5 (4): 38–42.
  8. Белоусов В.В., Ениколопов Г.Н., Мишина Н.М. Компартментализация передачи сигналов, опосредованных активными формами кислорода. Биоорганическая химия. 2013; 39 (4): 383.
  9. Dhabhar F.S. Effects of stress on immune function: the good, the bad and the beautiful. Immunol. Res. 2014; 58 (2–3): 193–210.
  10. Надеев А.Д., Зинченко В.П., Авдонин П.В., Гончаров Н.В. Токсические и сигнальные свойства активных форм кислорода. Токсикологический вестник. 2014; 2: 22–27.
  11. Milkovic L., Siems W., Siems R., Zarkovic N. Oxidative stress and antioxidants in cancerogenesis and integrative therapy of cancer. Curr. Pharm. Des. 2014; 20 (42): 6529–6542.
  12. Hussain S.P., Amstad P., He P., Robles A., Lupold S., Kaneko I., Ichimiya M., Sengupta S., Mechanic L., Okamura S., Hofseth L.J., Moake M., Nagashima M., Forrester K.S., Harris C.C. Р53- induced up-regulation of MnSOD and GPx but not catalase increases oxidative stress and apoptosis. Cancer Res. 2004; 64: 2350–2356.
  13. Мусин Ш.И., Фархутдинов Р.Р. Активные формы кислорода как маркеры метастатического поражения. Медицинский альманах. 2013; 27 (3): 118–119.
  14. Yang W, Zou L, Huang C, Lei Y. Redox regulation of cancer metastasis: molecular signaling and therapeutic opportunities. Drug Dev. Res. 2014; 75 (5): 331–341.
  15. Ayala A., Chung C.S., Lomas J.L., Song G.Y., Doughty L.A., Gregory S.H., Cioffi W.G., LeBlanc B.W., Reichner J., Simms H.H., Grutkoski P.S. Shock-induced neutrophil mediated priming for acute lung injury in mice: divergent effects of TLR-4 and TLR-4/FasL deficiency. Am. J. Pathol. 2002; 161 (6): 2283–2294.

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