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LIVER AND ITS LYMPH REGION AT BENZO[A]PYRENE EFFECTS IN AN EXPERIMENT
- Authors: Michurina S.V.1, Borodin Y.I.1, Kolesnikov S.I.2, Ishchenko I.Y.1, Konenkov V.I.1
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Affiliations:
- The Institute of Clinical and Experimental Lymphology, Novosibirsk, Russian Federation
- The Scientific Center for Family Health and Human Reproduction Problems, Irkutsk, Russian Federation
- Issue: Vol 70, No 2 (2015)
- Pages: 242-248
- Section: SHORT MESSAGES
- URL: https://vestnikramn.spr-journal.ru/jour/article/view/62
- DOI: https://doi.org/10.15690/vramn.v70i2.1319
- ID: 62
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Abstract
Background: Benzo[a]pyrene (BaP) is the widespread environmental toxicant with carcinogenic activity. BaP undergoes metabolic activation in the liver microsomal monooxygenase system, and its regional lymph nodes act as peripheral filters, purifying lymph formed in the liver. It remains an open question about the significance of the integral liver and lymphatic system work in supporting processes of adaptation and resistance to the BaP effects. Objective: The purpose of our investigation was to study structural and metabolic changes in the liver and its lymph region in males Wistar rats weighing 180–220 g. Methods: Animals of the experimental group (n =20) daily for 3 days was performed BaP injections: intraperitoneally with 2 mg per 100 g of body weight in 0.2–0.3 ml of olive oil. Rats in the control group (n =20) received in the same mode of injection of olive oil. The light and electron microscopy morphometric study of the liver and its regional lymph nodes morphometric analysis were performed. The intensity of lipid peroxidation in the liver was measured by the number of diene conjugates (DC), ketotriene conjugates (KC) and malondialdehyde (MDA). Results: Simultaneous increase of the hepatic sinusoids relative area and the specific area of the regional lymph nodes sinus system was discovered under the BaP effect. At ultrastructural level dilatation of the Disse spaces, filling of cell detritus and collagen fibers bundles of these spaces were noted. Damage of nuclear apparatus, balloonie expansion of granular endoplasmic reticulum profiles were found in hepatocytes, condensation of the mitochondrial matrix was observed. The relative squares of B-dependent zones increased and T-dependent paracortical zone reduced in the regional lymph nodes. The increase in the content of KC and MDA in liver was dentified. Conclusion: benzo[a]pyrene causes interrelated cascade of reactions in the liver and in its lymphatic region: a disturbance of the blood-lymph barrier morphological organization, the difficulty of lymph drainage from the organ. These promotes development of tissue hypoxia and apoptosis, protein synthesizing and energy cell apparatus disruptions, formation of lymphoid tissue temporary groups in the liver.
About the authors
S. V. Michurina
The Institute of Clinical and Experimental Lymphology, Novosibirsk, Russian Federation
Author for correspondence.
Email: S.Michurina@NGS.ru
доктор медицинских наук, профессор, главный научный сотрудник лаборатории функциональной морфологии лимфатической системы НИИ клинической и экспериментальной лимфологии
Адрес: 630117, Новосибирск, ул. Тимакова, д. 2, тел.: +7 (383) 335-98-52
Yu. I. Borodin
The Institute of Clinical and Experimental Lymphology, Novosibirsk, Russian Federation
Email: lympha@niikel.ru
академик РАН, директор Центра эндоэкологической реабилитации НИИ клинической и экспериментальной лимфологии
Адрес: 630117, Новосибирск, ул. Тимакова, д. 2, тел.: +7 (383) 335-93-53
S. I. Kolesnikov
The Scientific Center for Family Health and Human Reproduction Problems, Irkutsk, Russian Federation
Email: sikolesnikov2012@gmail.com
академик РАН, главный научный сотрудник НЦ ПЗСРЧ
Адрес: 664003, Иркутск, ул. Тимирязева, д. 16, тел.: +7 (3952) 20-76 36
I. Yu. Ishchenko
The Institute of Clinical and Experimental Lymphology, Novosibirsk, Russian Federation
Email: irenisch@mail.ru
кандидат биологических наук, старший научный сотрудник лаборатории функциональной
морфологии лимфатической системы НИИ клинической и экспериментальной лимфологии
Адрес: 630117, Новосибирск, ул. Тимакова, д. 2, тел.: +7 (383) 335-98-52
V. I. Konenkov
The Institute of Clinical and Experimental Lymphology, Novosibirsk, Russian Federation
Email: vikonenkov@gmail.com
академик РАН, директор НИИ клинической и экспериментальной лимфологии.
Адрес: 630117, Новосибирск, ул. Тимакова, д. 2, тел.: +7 (3833) 33-64-09
References
- Pessah I.N., Beltzner C., Burchiel S.W., Sridhar G., Penning T., Feng W. A bioactive metabolite of benzo(a)pyrene, benzo(a)pyrene 7,8-dione, selectively alters microsomal Ca2+ transport and ryano-dine receptor function. Mol. Pharmacol. 2001; 59 (3): 506–513.
- Pieters N., Koppen G., Smites K., Napierska D., Plusquin M., De Prins S., Van De Weghe H., Nelen V., Cox B., Cuypers A., Hoet P., Schoeters G., Nawrot T.S. Decreased mitochondrial DNA content in association with exposure to polycyclic aromatic hydrocarbons in house dust during wintertime: from a population enquiry to cell culture. PLoS One. 2013; 8 (5): 63208.
- Alexander J.S., Ganta V.C., Jordan P.A., Marlys H. Gastrointestinal lymphatics in health and disease. Pathophysiology. 2010; 17 (4): 315–335.
- Borodin Yu.I., Michurina S.V., Ishchenko I.Yu., Belkin A.D. Limfaticheskii region i gematolimfaticheskii bar'er pecheni v norme. V kn.: Limfologiya. Pod red. V.I. Konenkova, Yu.I. Borodina, M.S. Lyubarskogo [Lymphatic Part and Hematolymphatic Barrier of Liver in Health. In book: Lymphology. Edited by V.I. Konenkov, Yu.I. Borodin, M.S. Lyubarskii]. Novosibirsk, Manuskript, 2012. P. 215–220.
- Putilina F.E., Galkina O.V., Eshchenko N.D., Dizhe G.P., Krasovskaya I.E. Praktikum po svobodnoradikal'nomu okisleniyu [Workshop on Free Radical Oxidation]. St. Petersburg, SPbGU, 2006. P. 3–10.
- Greter M., Hofmann J., Becher B. Neo-lymphoid aggregates in the adult liver can initiate potent cell mediated immunity. PLoS Biol. 2009; 7 (5): 1000109.
- Michurina S.V. Influence of acute effects of 3,4-benzopyrene in the fat cells of the liver of rats of different sexes. Byulleten' SO RAMN = Bulletin of SD RAMS. 1995; 2: 66–69.
- Mouta C.C., Nasser S.M., di Tomaso E., Padera T.P., Boucher Y., Tomarev S.I., Jain R.K. LYVE-1 is not restricted to the lymph vessels: expression in normal liver blood sinusoids and down-regulation in human liver cancer and cirrhosis. Cancer Res. 2001; 61 (22): 8079–8084.
- Pupyshev A.B., Gutina E.M., Fedina R.G., Michurina S.V., Shurlygina A.V., Verbitskaya L.V. Effect of Benz(a)pyrene and Constant Light Exposure on Rat Liver Lysosomes and Biliary Excretion of Lysosomal Enzymes. Bull. Exp. Biol. Med. 2005; 139 (1): 34–37.
- Podechard N., Tekpli X., Catheline D., Holme J.A., Rioux V., Legrand P., Rialland M., Fardel O. Mechanisms involved in lipid accumulation and apoptosis induced by 1-nitropyrene in Hepa1c1c7 cells. Toxicol. Lett. 2011; 206 (3): 289–299.
- Barhoumi R., Mouneimne Y., Awooda I., Safe S., Donnelly K.C., Burghardt R.C. Characterization of calcium oscillations in normal and benzo(a)pyrene-treated clone 9 cells. Toxicol. Sci. 2002; 68 (2): 444–450.
- Kula B., Sobczak A. and Kuska R. Effects of static and ELF magnetic fields on free radical processes in rat liver and kidney. Electro-Magnetobiol. 2000; 19 (1): 99–105.
- Chernyak Yu.I., Grassman D.A., Kolesnikov S.I. Vliyanie stoikikh organicheskikh zagryaznitelei na biotransformatsiyu ksenobiotikov [Influence of Persistent Organic Pollutants in the Biotransformation of Xenobiotics]. Novosibirsk, Nauka, 2007. 134 p.
- Chernyak Yu.I., Kolesnikov S.I., Chernyak E.V. Tsitokhrom R450: osnovnye predstavleniya, metody issledovaniya, znachenie dlya prakticheskoi meditsiny [Cytochrome P450: Basic Concepts, Research Techniques, the Importance of Practical Medicine]. Irkutsk, IGU, 2014. 47 p.
- Borodin Yu.I., Maiborodin I.V., Safina A.F., Strunkin D.N. The Possibility of Cytochrome P450 1A1/1A2 Induction in Cells of Distant Lymph Nodes of Rats after Enteral Treatment with Benzo(a)pyrene. Bull. Exp. Biol. Med. 2005; 140 (5): 564–567.
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