Comparison Study of Oropharyngeal Microbiota in Case of Bronchial Asthma and Chronic Obstructive Pulmonary Disease in Different Severity Levels

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Abstract

Backgraund: The result of comparative study of oropharyngeal microbiota taxonomic composition in patients with different severity level of bronchial asthma (BA) and chronic obstructive pulmonary disease (COPD) is presented in this paper. 

Aims: To compare oropharyngeal microbiota composition in case of bronchial asthma and chronic obstructive pulmonary disease in different severity levels. 

Metods: 138 patients, 50 with BA and 88 with COPD were studied. For each patient was collected anamnesis vitae, swab from the back of the throat and performed physical examination. High-throughput 16S ribosomal RNA gene sequencing and bioinformatic analysis was employed to characterize the microbial communities. 

Results: As a result of the study was found a number of differences on various taxonomic levels in microbiota’s composition within group of patients with different severity level of BA and group of patients with different severity level of COPD and between those groups. COPD patients with GOLD 1–2 in comparison with GOLD 3–4 patiens are marked by prevalence of species Brevibacterium aureum, genus Scardovia, Coprococcus, Haemophilus, Moryella, Dialister, Paludibacter and decrease of Prevotella melaninogenica species. BA patients with severe uncontrolled asthma in comparison with patients which have mild persistent asthma are marked by decrease of Prevotella and increase of species Bifidobacterium longum, Prevotella nanceiensis, Neisseria cinerea, Aggregatibacter segnis and genus Odoribacter, Alloiococcus, Lactobacillus, Megasphaera, Parvimonas, Sneathia. Patient’s microbiota in BA group in comparison with COPD group is characterized by the prevalence of Prevotella melaninogenica and genus Selenomonas, Granulicatella и Gemella, and decrease of Prevotella nigrescens, Haemophilus influenza and genus Aggregatibacter, Alloiococcus, Catonella, Mycoplasma, Peptoniphilus и Sediminibacterium. There are no differences between microbiota composition in case of severe uncontrolled BA and very severe COPD. 

Conclusion: Lack of differences in oropharyngeal microbiota taxonomic composition between patients with severe uncontrolled BA and very severe COPD allow us to suggest a similarity of bronchopulmonary system condition in case of diseases' severe stages.

About the authors

Ludmila Mikhailovna Ogorodova

Siberian State Medical University

Email: lm-ogorodova@mail.ru
доктор медицинских наук, профессор, член-корреспондент РАМН, заведующая кафедрой факультетской педиатрии с курсом детских болезней лечебного факультета ГБОУ ВПО «Сибирский государственный медицинский университет» Минздрава России, заместитель министра науки и образования Российской Федерации Russian Federation

Sergey Vyacheslavovich Fedosenko

Siberian State Medical University

Author for correspondence.
Email: s-fedosenko@mail.ru
кандидат медицинских наук, докторант кафедры госпитальной терапии с курсом физической реабилитации и спортивной медицины ГБОУ ВПО «Сибирский государственный медицинский университет» Минздрава России Russian Federation

Anna Sergeevna Popenko

Scientific Research Institute of Physical-Chemical Medicine

Email: kirika77@gmail.com
лаборант лаборатории биоинформатики ФГБУН «Научно-исследовательский институт физико-химической медицины» ФМБА России Russian Federation

Vyacheslav Alekseevich Petrov

Siberian State Medical University

Email: vyacheslav.a.petrov@mail.ru

младший научный сотрудник центральной научно-исследовательской лаборатории ГБОУ ВПО «Сибирский государственный медицинский университет» Минздрава России 

Russian Federation

Alexandr Viktorovich Tyakht

Scientific Research Institute of Physical-Chemical Medicine

Email: at@niifhm.ru
кандидат биологических наук, младший научный сотрудник лаборатории биоинформатики ФГБУН «Научно-исследовательский институт физико-химической медицины» ФМБА России Russian Federation

Irina Vladimirovna Saltykova

Siberian State Medical University

Email: ira.salticova@mail.ru
кандидат медицинских наук, научный сотрудник Центральной научно-исследовательской лаборатории ГБОУ ВПО «Сибирский государственный медицинский университет» Минздрава России Russian Federation

Ivan Anatolievich Deyev

Siberian State Medical University

Email: ivandeyev@yandex.ru
доктор медицинских наук, ассистент кафедры факультетской педиатрии с курсом детских болезней лечебного факультета ГБОУ ВПО «Сибирский государственный медицинский университет» Минздрава России Russian Federation

Evgeny Sergeevich Kulikov

Siberian State Medical University

Email: evgeny.s.kulikov@gmail.com
доктор медицинских наук, доцент кафедры общей врачебной практики и поликлинической терапии ГБОУ ВПО «Сибирский государственный медицинский университет» Минздрава России Russian Federation

Natalia Aleksandrovna Kirillova

Siberian State Medical University

Email: kirillova@mail.ru
кандидат медицинских наук, ассистент кафедры общей врачебной практики и поликлинической терапии ГБОУ ВПО «Сибирский государственный медицинский университет» Минздрава России Russian Federation

Vadim Markovich Govorun

Scientific Research Institute of Physical-Chemical Medicine;
Kazan Federal University

Email: niifhm@fmbamail.ru
доктор биологических наук, профессор, член-корреспондент РАН, заместитель директора по научной работе ФГБУН «Научно-исследовательский институт физико-химической медицины» ФМБА России, главный научный сотрудник научно-исследовательской лаборатории «Омиксные технологии» Института фундаментальной медицины и биологии Казанского (Приволжского) федерального университета Russian Federation

Elena Sergeevna Kostryukova

Scientific Research Institute of Physical-Chemical Medicine;
Kazan Federal University

Email: el-es@yandex.ru

 

 

Russian Federation

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