THE DEVELOPMENT OF TOTAL COMPLEMENT ACTIVITY ASSAY BASED ON COMPLEMENT-DEPENDENT IMMUNE LIPOSOME LYSIS

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Abstract

Background: The purpose of work was development of a fast and reproduced procedure for measurement of the total complement activity (ТСА) in human or animal blood serum. Materials and methods: Steady at storage liposomes preparations, which surface sensitized 2,4-DNP haptens, and the internal volume contains calceine or sulforhodamine 101 are obtained. Complement-dependent immune lysis of liposomes at presence of the anti-2,4-DNP immunoglobulines and complement preparations from animals are investigated. Results: It is shown that the degree of liposomes immune lysis depends on complement concentration in a wide range that can be used for definition of TCA level. Research of blood sera from patients has revealed correlation (r =0,793) between data received with the help of liposome immunolytic systems, and the data of nephelometric analysis with application of suspension sheep erythrocytes. Conclusion: The method allows to define total complement activity in blood serum in 15 minutes without separation of reaction components. This might be useful for measurement ТСА level at patients with various diseases and realization of scientific researches.

About the authors

S. N. Skopinskaya

State Scientific Research Institute of Biological Engineering, Moscow, Russian Fеderation

Author for correspondence.
Email: diasol@dol.ru

кандидат биологических наук, ведущий научный сотрудник отдела спектральных методов анализа ГосНИИ биологического приборостроения
Адрес: 125424, Москва, Волоколамское ш., д. 75, тел.: (495) 491-86-83

Russian Federation

S. P. Yarkov

State Scientific Research Institute of Biological Engineering, Moscow, Russian Fеderation

Email: diasol@dol.ru

доктор биологических наук, начальник отдела спектральных методов анализа ГосНИИ биологического приборостроения
Адрес: 125424, Москва, Волоколамское ш., д. 75, тел.: (495) 491-86-83

Russian Federation

E. N. Khramov

State Scientific Research Institute of Biological Engineering, Moscow, Russian Fеderation

Email: niibp@dol.ru
доктор технических наук, профессор, директор ГосНИИ биологического приборостроения
Адрес: 125424, Москва, Волоколамское ш., д. 75, тел.: (495) 491-73-72 Russian Federation

A. V. Antashev

Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow, Russian Fеderation

Email: artem72antashev@mail.ru

кандидат медицинских наук, врач-лаборант ФНЦ трансплантологии и искусственных органов им. акад. В.И. Шумакова, врач-лаборант Клинической больницы № 1 Управления делами Президента РФ Адрес: 123182, Москва, ул. Щукинская, д. 1, тел.: (495) 491-35-05

Russian Federation

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