CHEMILUMINESCENT AND ENZYME ACTIVITY OF NEUTROPHILS IN PATIENTS WITH WIDESPREAD PURULENT PERITONITIS DEPENDING ON THE OUTCOME OF DISEASE

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Abstract

Background: Aim was to study the chemiluminescent activity and the activity of NAD- and NADP-dependent dehydrogenases of neutrophils
depending on the outcome of the widespread purulent peritonitis (WPP). Patients and methods: 51 patients with a mean age of RSE 54,2±19,2 years were observed. As a control 75 healthy people of similar age range were examined. Lucigenin- and luminol-dependent chemiluminescence activity and activity of NAD(P)-dependent dehydrogenases peripheral blood neutrophils were studied. Results: In patients with a favorable outcome of WPP the maximum intensity increased and the magnitude of the activation index of lucigenine-dependent spontaneous chemiluminescence reduced.
Regardless to the outcome of WPP in patients with increased activation index and maxima of the luminol-dependent spontaneous and zymosaninduced chemiluminescence. At the unfavorable outcome of the disease in neutrophils NAD- dependent isositrate dehydrogenase and anaerobic lactate dehydrogenase reaction activity increased. Regardless to the outcome WPP in patients neutrophils aerobic reaction of lactate dehydrogenase, glucose-6-phosphate dehydrogenase and NADP-dependent glutamate dehydrogenase activity reduced but levels of NADH-dependent reaction of malate dehydrogenase, NADH-dependent glutamate dehydrogenase and NADP-dependent isocitrate dehydrogenase activity increased. Conclusion: With the reduction of the intensity of plastic processes and imbalances enzymatic activity in nitrogen metabolism in patients with WPP at unfavorable outcome of the disease increases the activity of enzymes that characterize the level of anaerobic and aerobic respiration. In the absence of marked changes in the activity of enzymes that characterize the level of energy processes in cells of patients with favorable outcome of the WPP, increases the intensity of spontaneous lucigenin-dependent chemiluminescence and reduced neutrophil activation index.

About the authors

A. A. Savchenko

Institute of Medical Problems of the North, Krasnoyarsk
Prof. V.F. Voyno-Yasenetsky Krasnoyarsk State Medical University

Author for correspondence.
Email: aasavchenko@yandex.ru
PhD,  professor, Head of the Laboratory of Molecular cell physiology and pathology of FSBI “Scientific research institute of medical problems of the North”, Head of A.T. Pshonik Department of  Physiology of V.F.Voino-Yasenetskii Krasnoyarsk State Medical University Russian Federation

D. E. Zdzitovetskii

V.F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Russian Federation

Email: zdz64@mail.ru
MD, assistant professor, Head of Yu.M. Lubenskii Department of Surgery Diseases of V.F.Voino-Yasenetskii Krasnoyarsk State Medical University. Address: 3G, Partizana Zheleznyaka Street, Krasnoyarsk, RF, 660022; tel.: +7 (391) 246-94-06 Russian Federation

A. G. Borisov

Institute of Medical Problems of the North, Krasnoyarsk, Russian Federation

Email: 2885263@mail.ru
MD, leading research scientist of the Laboratory of Molecular cell physiology and pathology of FSBI “Scientific research institute of medical problems of the North”. Address: 3G, Partizana Zheleznyaka Street, Krasnoyarsk, RF, 660022; tel.: +7 (391) 228-06-83 Russian Federation

N. A. Luzan

Institute of Medical Problems of the North, Krasnoyarsk, Russian Federation

Email: laskimo@mail.ru
junior research scientist of the Laboratory of Molecular cell physiology and pathology of FSBI “Scientific research institute of medical problems of the North”. Address: 3G, Partizana Zheleznyaka Street, Krasnoyarsk, RF, 660022; tel.: +7 (391) 228-06-83 Russian Federation

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