SELECTIVE EFFECTS OF PULMONARY SURFACTANT ON VARIOUS SUBPOPULATIONS OF ALVEOLAR MACROPHAGES IN THE MODEL OF EXPERIMENTAL TUBERCULOSIS

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Abstract

Pulmonary surfactant is necessary component for maintenance of high level of phagocytic activity of alveolar macrophages. Tuberculosis inflammation reduces the production of surfactant by type II cells and phagocytic activity of alveolar macrophages. The effects of exogenous pulmonary surfactant on the ultrastructural changes of various subpopulations of alveolar macrophages were studied by TEM-method. For investigations the bronchial alveolar lavage fluid from guinea pigs infected of M. tuberculosis and treated by isoniazid or isoniazid + exogenous pulmonary surfactant were used. It was shown that isoniazid + exogenous pulmonary surfactant normalizes the heterogeneous population of alveolar macrophages providing stimulating effects on their maturation and phagocytic activity more effectively than isoniazid therapy.

 

About the authors

V. V. Erokhin

Central Tuberculosis Research Institute of Russian Academy of Medical Sciences, Moscow, Russian Federation

Author for correspondence.
Email: cniit@ramn.ru
профессор, член-корреспондент РАМН, директор ФГБУ «ЦНИИТ» РАМН Адрес: 107564, Москва, Яузская аллея, д. 2 Тел.: (499) 785-91-19 Russian Federation

L. N. Lepekha

Central Tuberculosis Research Institute of Russian Academy of Medical Sciences, Moscow, Russian Federation

Email: lep3@yandex.ru
доктор биологических наук, профессор, заведующая лабораторией патоморфологии ФГБУ «ЦНИИТ» РАМН Адрес: 107564, Москва, Яузская аллея, д. 2 Тел.: (499) 785-91-79 Russian Federation

M. V. Erokhina

Central Tuberculosis Research Institute of Russian Academy of Medical Sciences, Moscow, Russian Federation
Lomonosov Moscow State University, Russian Federation

Email: erokhinam@bk.ru
кандидат биологических наук, старший научный сотрудник кафедры клеточной био- логии и гистологии биологического факультета МГУ им. М.В. Ломоносова, старший научный сотрудник лабора- тории патоморфологии ФГБУ «ЦНИИТ» РАМН Адрес: 119991, Москва, ГСП-1, Ленинские горы, д. 1 Тел.: (495) 939-45-67, (499) 785-91-79 Russian Federation

I. V. Bocharova

Central Tuberculosis Research Institute of Russian Academy of Medical Sciences, Moscow, Russian Federation

Email: 3595598@mail.ru
кандидат биологических наук, заведующая виварием ФГБУ «ЦНИИТ» РАМН Адрес: 107564, Москва, Яузская аллея, д. 2 Тел.: 499-780-47-14 Russian Federation

A. V. Kurynina

Lomonosov Moscow State University, Russian Federation

Email: frassy@mail.ru
ассистент кафедры клеточной биологии и гистологии биологического факультета МГУ им. М.В. Ломоносова Адрес: 119991, Москва, ГСП-1, Ленинские горы, д. 1 Тел.: (495) 939-45-67 Russian Federation

G. E. Onishchenko

Lomonosov Moscow State University, Russian Federation

Email: galina22@mail.ru
доктор биологических наук, профессор, заведующая кафедрой клеточной биологии и гистологии биологического факультета МГУ им. М.В. Ломоносова Адрес: 119991, Москва, ГСП-1, Ленинские горы, д. 1 Тел.: (495) 939-17-94 Russian Federation

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