Annals of the Russian academy of medical sciencesAnnals of the Russian academy of medical sciences0869-60472414-3545"Paediatrician" Publishers LLC34710.15690/vramn.v67i2.124OUTBREAK OF ACUTE ENTEROVIRUS INTESTINAL INFECTION IN SAKHALIN REGION IN AUGUST 2010DeminaA. V.deminaanna@mail.ruTernovoiV. A.tern@vector.nsc.ruDarizhapovB. B.sakhnadzor@sakhalin.ruYakubichT. V.sakhfguz@sakhfguz.ruSementsovaS. A.sementsova@vector.nsc.ruDeminaO. K.demina@vector.nsc.ruProtopopovaE. V.eprotopopova@ngs.ruLoktevV. B.loktev@vector.nsc.ruAgafonovA. P.agafonov@vector.nsc.ruNetesovS. V.nauka@nsu.ruFSRE State Research Center of Virology and Biotechnology VECTORDepartment of Federal Service for Supervision of Consumer Rights Protection and Human Welfare in the Sakhalin regionCenter of Hygiene and Epidemiology in the Sakhalin regionFSRE State Research Center of Virology and Biotechnology VECTOR
Novosibirsk State University22022012672646807082015Copyright © 2012, "Paediatrician" Publishers LLC2012<em>The investigation of cases of acute intestinal infections in the Sakhalin region of Russia in August, 2010 is described. Epidemiological and molecular biological studies were conducted. After initial PCR screening and determining the nucleotide sequences of the positive samples the following enteroviruses were found: Coxsackie A2 — 42 samples (45%), Coxsackie A4 — 31 sample (34%), Enterovirus 71 — 6 samples (6,5%), Coxsackievirus B5 — 6 samples (6,5%), Coxsackie B3 — 4 samples (4%) and Coxsackie B1 — 4 samples (4%). The phylogenetic analysis of sequences showed that the closest analogues for the nucleotide sequences of these genotypes were previously identified in Japan, Korea and China in 2000–2010.</em><br /><br />acute intestinal infectionenterovirusСoxsackievirus A2, A4острая кишечная инфекцияэнтеровирусыКоксаки А2, А4[1. Pallansch M.A., Roos R.P. Enteroviruses: polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses. Fields’ Virology. (Ed. D.N. Knipe et al.) 5th Ed. Philadelphia, Lippincott Williams & Wilkins, 2007. pp. 839–893.][2. Available at: http://www.interlabservice.ru/catalog/reagents/index.php?sid=678][3. Available at: http://www.ncbi.nlm.nih.gov/][4. Kumar S., Nei M., Dudley J., Tamura K. MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief. Bioinform. 2008; 9: 299−306.][5. Felsenstein J. Confidence Limits on Phylogenies: An Approach Using the Bootstrap. Evolution. 1985; 39: 783−791.][6. Miyamura K., Nishimura Y., Abo M. et al. Adaptive mutations in the genomes of enterovirus 71 strains following infection of mouse cells expressing human P-selectin glycoprotein ligand-1. J. Gen. Virol. 2011; 92 (2): 287−291.][7. He W., Lu H., Song D. et al. The evidence of Coxsackievirus B3 induced myocarditis as the cause of death in a Sichuan snub-nosed monkey (Rhinopithecus roxellana). J. Med. Primatol. 2009; 38 (3): 192−198.]