Annals of the Russian academy of medical sciencesAnnals of the Russian academy of medical sciencesВестник Российской академии медицинских наук0869-60472414-3545"Paediatrician" Publishers LLC21610.15690/vramn.v68i2.550SHORT MESSAGESКРАТКИЕ СООБЩЕНИЯAUTOCHTHONOUS PROBIOTICS IN PREVENTION OF INFECTIOUS AND INFLAMMATORY DISEASES OF A HUMAN IN THE ALTERED HABITATSАУТОПРОБИОТИКИ КАК СРЕДСТВО ПРОФИЛАКТИКИ ИНФЕКЦИОННО-ВОСПАЛИТЕЛЬНЫХ ЗАБОЛЕВАНИЙ У ЧЕЛОВЕКА В ИСКУССТВЕННОЙ СРЕДЕ ОБИТАНИЯIl'inV. K.ИльинВ. К.
Russian Federation

PhD, Professor, head of the department of sanitary and hygienic human security in artificial environment, Institute of Biomedical Problems RAS Address: 123007, Moscow, Khoroshevskoe Highway, 76a; tel.: (499) 195-65-54

ilyin@imbp.ruSuvorovA. N.СуворовА. Н.

PhD, Professor, Head of the Department of Molecular Microbiology, Research Institute of Experimental Medicine RAMS Address: 197376, St. Petersburg, Acad. Pavlova St., 12; tel.: (812) 234-93-19

alexander_suvorov1@hotmail.ruKiryukhinaN. V.КирюхинаН. В.
Russian Federation

PhD, Research Worker, Laboratory of Microbial Human Ecology, Institute of Biomedical Problems RAS Address: 123007, Moscow, Khoroshevskoe Highway, 76a; tel.: (499) 195-68-36

kiryukhina_nataliya@hotmail.comUsanovaN. A.УсановаН. А.
Russian Federation

Research Worker, Laboratory of Microbial Human Ecology, Institute of Biomedical Problems RAS Address: 123007, Moscow, Khoroshevskoe Highway, 76a; tel.: (499) 195-68-36

usanova@imbp.ruStarkovaL. V.СтарковаЛ. В.
Russian Federation

PhD, Research Worker, Laboratory of Microbial Human Ecology, Institute of Biomedical Problems RAS Address: 123007, Moscow, Khoroshevskoe Highway, 76a; tel.: (499) 195-68-36

starkova@imbp.ruBoyarintsevV. V.БояринцевВ. В.
Russian Federation

PhD,Professor, Head Physician of Clinical Hospital No. 1 (Volynskaya) Department for Presidential Affairs of the Russian Federation Address: 121352, Moscow, Starovolynskaya St., 10; tel.: (495) 441-80-01

wpx@mail.ruKarasevaA. B.КарасеваА. Б.
Russian Federation

Research Worker, Department of Molecular Microbiology, Research Institute of Experimental Medicine RAMS Address: 197376, St. Petersburg, Acad. Pavlova St., 12; tel.: (812) 234-93-19

tarno@list.ruState scientific center of Russian Federation — Institute of Biomedical Problems of RAS, Moscow, Russian FederationГосударственный научный центр Российской Федерации — Институт медико-биологических проблем РАН, Москва, Российская ФедерацияInstitute of Experimental Medicine of the North-West Branch of the Russian Academy of Medical Sciences, St.Petersburg, Russian FederationНаучно-исследовательский институт экспериментальной медицины, Санкт-Петербург, Российская ФедерацияClinical Hospital № 1 (Volynskaya), Moscow, Russian FederationКлиническая больница № 1 (Волынская) управления делами Президента РФ, Москва, Российская Федерация22022013682Vestnik Rossiiskoi akademii medetsinskikh nauk / Annals of the Russian academy of medical sciencesВестник Российской академии медицинских наук566207082015Copyright ©; 1970, "Paediatrician" Publishers LLCCopyright ©; 1970, Издательство "Педиатръ"1970"Paediatrician" Publishers LLCИздательство "Педиатръ"https://vestnikramn.spr-journal.ru/jour/about/submissionshttps://vestnikramn.spr-journal.ru/jour/article/view/216

Dysbiotic shifts in intestinal and pharyngeal microflora were studied in 22 normal volunteers in 9-, 14-, 105- and 520-d chamber experiments simulating some of the spaceflight factors. Two preparations were administered to prevent pharyngeal and intestinal dysbiosis: oral dry probiotic based on indigenous intestinal Enterococci and topical collagen-immobilized Lactobacterin based on indigenous intestinal Lactobacilli. Topical autoprobiotic lactobacterin reduced the growth of opportunistic pathogens in the throat during the experiments. Oral autoprobiotic based on Enterococci reduced the content of intestinal opportunistic pathogens, supporting the high level of protecting microflora. Most of autostrains are free from pathogenicity factors, nevertheless, implementation of genetic testing of indigenous strains are reasonable.

Изучались дисбиотические сдвиги микрофлоры кишечника и глотки у 22 здоровых добровольцев в 9-, 14-, 105- и 520-суточных экспериментах в герметично замкнутом объекте, моделирующих воздействие факторов космического полета. Для предотвращения дисбактериоза кишечника и глотки были использованы 2 препарата: пероральный препарат, созданный на основе аутоштаммов кишечных энтерококков, и местный препарат на основе аутоштаммов кишечных лактобацилл лактобактерин, иммобилизованнный на коллагене. Местный препарат аутоштаммов лактобацилл предотвращал рост условно-патогенной микрофоры глотки в течение эксперимента. Пероральный препарат аутоштаммов энтерококков способствовал снижению содержания условно-патогенной микрофлоры в течение эксперимента, одновременно поддерживая высокий уровень защитной микрофлоры кишечника. Генетические исследования показали, что большинство аутоштаммов свободно от генов, кодирующих факторы патогенности. Тем не менее, наличие небольшого числа штаммов, имеющих эти гены, доказывает целесообразность проведения предварительных генетических тестов.

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