Annals of the Russian academy of medical sciencesAnnals of the Russian academy of medical sciences0869-60472414-3545"Paediatrician" Publishers LLC19810.15690/vramn.v68i4.613INVESTIGATION OF POSSIBLE APPROACHES TO ANGIOGENESIS REGULATION IN VITRO WITH THE HELP OF RECOMBINANT FRAGMENTS OF ANGIOGENESIS INHIBITORS SUCH AS ENDOSTATIN, TUMSTATIN AND PEDFKuznetsovS. L.RAMS cor. member, Head of the Department of Innovative Development of Science Russian Academy of Medical Sciences Address: 109240, Moscow, Soljanka St., 14; tel.: 8(495) 698-56-31kuznetsov@ramn.ruLikhvantsevaV. G.PhD, Professor, Department of Fundamental Medicine Lomonosov Moscow State University Address: 119192, Moscow Lomonosov Ave, 31/5; tel: (495) 932-88-14info@fbm.msu.ruArutyunyanE. V.Ophthalmologist, City Hospital in Yubileiny Address: 141090, Moscow region, Yubileiny, Lesnaya St., 8/10; tel.: 8(498)-646-96-80ev1012@ya.ruKuz'minK. A.Pathologist, Pathology Department of the Central Clinical Hospital Russian Academy of Sciences Address: 119333, Moscow, Litovskiy Blvd, 1A; tel.: (495) 921-34-74info@fbm.msu.ruSel'kovS. A.PhD, Professor, Head of the Immunology Laboratory, Research Institute of Obstetrics and Gynecology named after D.O. Otta Address: 199034, St. Petersburg, Mendeleev line 3; tel.: (812) 328-14-02iagmail@ott.ruSokolovD. I.PhD, Head of the Group of Angiogenesis Study of Immunology Laboratory, Research Institute of Obstetrics and Gynecology named after D.O. Otta Address: 199034, St. Petersburg, Mendeleev line 3; tel.: (812) 328-14-02iagmail@ott.ruSechenov First Moscow State Medical University, Russian FederationLomonosov Moscow State University, Russian FederationD.O. Ott Research Institute of Obstetrics and Gynecology, St. Petersburg, Russian Federation14042013684636707082015Copyright © 1970, "Paediatrician" Publishers LLC1970<p class="Text05"><em>Neovascular diseases of visual organ such as age-related macular degeneration, retinopathy of prematurity, diabetic retinopathy, thrombosis of central retina vein and its branches, neovascular glaucoma, choroid and retina tumors have the leading positions in the list of ophtalmopatologies that result in blindness and incapacity. The variety of angiostatic medications of applied ophtalmology is scant. The aim of work was to study the possibile approaches to angiogenesis regulation in vitro with the help of recombinant fragments of natural inhibitors of angiogenesis such as endostatin, tumstatin and PEDF (pigment epithelial derived factor), and also their ability to be the base of potentially feasible and pharmacologically active substances. It is determined that endostatin, tumstatin and PEDF, as well as the comparison medication Bevacizumab in vitro have pro- or antiangiogenic influence. The direction of the biological effect depends on the cultivation conditions, peptide concentration in the cultural fluid and stage of angiogenesis.</em></p><p class="Text05"> </p>neovascular diseases of visual organinhibitors and stimulators of angiogenesisendostatintumstatinPEDFнеоваскулярные заболевания органа зрениястимуляторы и ингибиторы ангиогенезаэндостатинтумстатинPEDF[1. Zhukov N.V. The current state of antianioennoy therapy. Target therapy without a target? Prakt. onkol. = Practical oncology. 2007; 8 (3): 164–170.][2. Ziangirova G.G., Likhvantseva V.G. Osobennosti i rol' aniogeneza v opukholyakh. V knige: Opukholi sosudistogo trakta glaza [Features and Angiogenesis Role in Tumors. In: Tumors of Vascular Tract of the Eye]. Moscow, Poslednee slovo, 2003. 456 p.][3. Ellington A.D., Szostak J.W. 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