Annals of the Russian academy of medical sciencesAnnals of the Russian academy of medical sciencesВестник Российской академии медицинских наук0869-60472414-3545"Paediatrician" Publishers LLC19510.15690/vramn.v68i4.610TRANSPLANTOLOGY: CURRENT ISSUESАКТУАЛЬНЫЕ ВОПРОСЫ ТРАНСПЛАНТОЛОГИИCORRECTION OF CRONIC LIVER FAILURE BY TRANSPLANTATION OF LIVER CELLS SUSPENSION AND CELL-ENGINEERING DESIGNS (EXPERIMENTAL INVESTIGATION)КОРРЕКЦИЯ ХРОНИЧЕСКОЙ ПЕЧЕНОЧНОЙ НЕДОСТАТОЧНОСТИ ПРИ ТРАНСПЛАНТАЦИИ КЛЕТОК ПЕЧЕНИ В ВИДЕ СУСПЕНЗИИ И КЛЕТОЧНО-ИНЖЕНЕРНЫХ КОНСТРУКЦИЙ (ЭКСПЕРИМЕНТАЛЬНОЕ ИССЛЕДОВАНИЕ)Got'eS. V.ГотьеС. В.
Russian Federation

RAMS academician, PhD, Professor, Director of Federal State Budgetary Institution of Transplantology and Artificial Organs named after acad. V.I. Shumakov, Head of the Department of Transplantology and Artificial Organs Federal State Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 196-18-03

viksev@yandex.ruShagidulinM. Yu.ШагидулинМ. Ю.
Russian Federation

PhD, Head of the Department of Experimental Transplantology and Artificial Organs, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov, Associate Professor of Transplantology and Artificial Organs Federal State Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 196-87-90

dr.shagidulin@mail.ruOnishchenkoN. A.ОнищенкоН. А.
Russian Federation

PhD, Professor, Head of Stem Cells Laboratory, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov, Department of Transplantology and Artificial Organs Federal State Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-45-31

viksev@yandex.ruKrashennikovM. E.КрашенинниковМ. Е.
Russian Federation

PhD, Senior Research Worker, Laboratory of Stem Cells, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-45-31

krashen@rambler.ruIl'inskiiI. M.ИльинскийИ. М.
Russian Federation

PhD, Professor, Head of the Department of Clinical Pathology, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov, Department of Transplantology and Artificial Organs Federal State Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-64-28

viksev@yandex.ruMozheikoN. P.МожейкоН. П.
Russian Federation

PhD, Physician, Pathology Department, Federal state budgetary institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-64-28

npm_72@mail.ruLyundupA. V.ЛюндупА. В.
Russian Federation

PhD, Head of the Department of Biomedical Research, Research Institute of Molecular Medicine, Federal State Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University. Address: 117997, Moscow, Trubetskaya St. 8; tel.: (495) 609-14-00 ext 3051

gma.tulkas@gmail.comVolkovaE. A.ВолковаЕ. А.
Russian Federation

Head of the Laboratory of Preparation and Carrying out of Experimental Studies, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov. Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-77-05

volckowa.g2011@yandex.ruPetrakovK. I.ПетраковК. И.
Russian Federation

Junior Research Worker, Cardiac Surgery Department №3, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov. Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-64-28

viksev@yandex.ruAvramovP. V.АврамовП. В.
Russian Federation

Head of the Experimental Laboratory with Vivarium, Federal State Budgetary Institution Federal Research Center of Transplantology and Artificial Organs named after Acad. V.I. Shumakov. Address: 123182, Moscow, Shukinskaya St. 1; tel.: (499) 190-64-28

viksev@yandex.ruPerovaN. V.ПероваН. В.
Russian Federation
viksev@yandex.ruSevast'yanovV. I.СевастьяновВ. И.
Russian Federation
viksev@yandex.ruFederal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian Federation I.M. Sechenov Moscow State Medical University, Moscow, Russian FederationФНЦ трансплантологии и искусственных органов им. акад. В.И. Шумакова, Москва, Российская Федерация Первый Московский государственный медицинский университет им. И.М. Сеченова, Российская ФедерацияFederal V. Shumakov Research Center of Transplantology and Artificial Organs. Moscow, Russian FederationФНЦ трансплантологии и искусственных органов им. акад. В.И. Шумакова, Москва, Российская ФедерацияI.M. Sechenov Moscow State Medical University, Moscow, Russian FederationПервый Московский государственный медицинский университет им. И.М. Сеченова, Российская Федерация14042013684Vestnik Rossiiskoi akademii medetsinskikh nauk / Annals of the Russian academy of medical sciencesВестник Российской академии медицинских наук445107082015Copyright ©; 1970, "Paediatrician" Publishers LLCCopyright ©; 1970, Издательство "Педиатръ"1970"Paediatrician" Publishers LLCИздательство "Педиатръ"https://vestnikramn.spr-journal.ru/jour/article/view/195

On an experimental model of chronic fibrotic liver damage (male rats Wistar (n=60), damage of CCl4, the duration of the experiment 90 days) it was studied the effectiveness of cell therapy for the correction of chronic liver failure. These rats were divided into 3 experimental groups: in the Ist group (control, n=10) isotonic saline (650 mkl.) was injected; in the IInd group (n=20) suspension of liver cells was applicated in a dose 8-10×106 cells; in the IIIrd group (n=30) suspension of liver cells and bone marrow cells (mesenchymal stromal cells) in ratio 5:1 were used as cell associates on microparticles injectable heterogeneous biopolymer hydrogel «SpheroGEL» (cell-engineering design) in common dose 8-10×106 cells . It was ascertained that in the 2nd and in the 3rd groups the accelerated normalization of disturbed liver functional indices (ALT, AST, ALP) took place – to 30 days, but in the control group only to 90 days. The reliable differences in rats of normalization of functional indices were absent between the IInd and the IIIrd groups. But in 90 days by using special histological dyeing it was found out that defibrotic processes in liver tissue were more expressed in the IIIrd group in comparison with the IIInd group. Received results were consequence of prolonged vital activity of cells (liver cells and mesenchymal stromal bone marrow cells) into cell-engineering designs, which were transplanted in the IIIrd group. The obtained effect can be explained by that the developed cell-engineering designs provide adequate conditions for prolonged vital activity of the transplanted cells.

(Авторы: С.В. Готье,М.Ю. Шагидулин, Н.А. Онищенко, М.Е. Крашенинников, И.М. Ильинский, Н.П. Можейко, А.В. Люндуп, Е.А. Волкова, К.И. Петраков, П.В. Аврамов, Н.В. Перова, В.И. Севастьянов)

На экспериментальной модели хронического фиброзирующего повреждения печени (крысы-самцы породы Вистар (n=60), затравка CCl4, длительность эксперимента 90 суток) изучена эффективность клеточной терапии при коррекции хронической печеночной недостаточности. Выполнено 3 группы опытов: I группа (n=10) – контрольная (введение физиологического раствора); II группа (n=20) – введение в печень суспензии клеток донорской печени в дозе 8-10×106 клеток; III группа (n=30) – введение в печень ассоциатов клеток донорской печени и донорских мультипотентных мезенхимальных стромальных клеток костного мозга в соотношении 5:1 и в суммарной дозе 8-10×106 клеток на микрочастицах инъекционного гетерогенного биополимерного гидрогеля СфероГЕЛЬ (клеточно-инженерные конструкции). Установлено, что клеточная терапия во II и III группах опытов способствовала достоверно ускоренной нормализации нарушенных функций печени: к 30 суткам вместо 90 суток в контроле (I группа). При этом достоверные различия в темпе нормализации функциональных показателей печени во II и III группах отсутствовали. Однако гистологический анализ показал, что через 90 суток темп дефиброзирования ткани печени в III группе был существенно более выражен, чем во II группе. Полученный эффект можно объяснить тем, что разработанные клеточно-инженерные конструкции обеспечивают адекватные условия для пролонгированной жизнедеятельности трансплантированных клеток.

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