Annals of the Russian academy of medical sciencesAnnals of the Russian academy of medical sciencesВестник Российской академии медицинских наук0869-60472414-3545"Paediatrician" Publishers LLC18810.15690/vramn.v68i5.665SHORT MESSAGESКРАТКИЕ СООБЩЕНИЯIMMUNOPHENOTYPIC CHARACTERISTIC OF DENDRITIC CELLS IN BRONCHIAL ASTHMA IN CONDITIONS OF EXTRACT OPISTHORCHIS FELINEUS IN VITROИММУНОФЕНОТИПИЧЕСКАЯ ХАРАКТЕРИСТИКА ДЕНДРИТНЫХ КЛЕТОК ПРИ БРОНХИАЛЬНОЙ АСТМЕ В УСЛОВИЯХ ВОЗДЕЙСТВИЯ ЭКСТРАКТОМ OPISTHORCHIS FELINEUS IN VITROKremerE. E.КремерЕ. Э.
Russian Federation

PhD, Research Worker, Central Research Laboratory, Siberian State Medical University Address: 634050, Tomsk, Moscovsky Trakt, 2/18; tel.: (3822) 52-83-97

elenakremer@yandex.ruOgorodovaL. M.ОгородоваЛ. М.
Russian Federation

RAMS cor. member, Professor, Head of Department of Pediatric Faculty with a Course of Children Diseases of the Medical faculty, Siberian State Medical University Address: 634050, Tomsk, Moscovsky Trakt, 2; tel.: (3822) 53-10-22

lm-ogorodova@mail.ruKirillovaN. A.КирилловаН. А.
Russian Federation

PhD, Assistant, The Department of Hospital Therapy with Course of Physical Rehabilitations and Sports Medicine, Siberian State Medical University Address: 634063, Tomsk, street Chernykh, 96; tel.: (3822) 64-44-81

kirillova.natalya@gmail.comKhvorilovaK. V.ХвориловаК. В.
Russian Federation

Junior Research Worker, Central Research Laboratory, Siberian State Medical University Address: 634050, Tomsk, Moscovsky Trakt, 2/18; tel.: (3822) 52-83-97

kseniahvorilova@gmail.comPerevozchikovaT. V.ПеревозчиковаТ. В.
Russian Federation

PhD, Research Worker, Central Research Laboratory, Siberian State Medical University. Address: 634050, Tomsk, Moscovsky Trakt, 2/18; tel.: (3822) 52-83-97

perevozchikova.tv@gmail.comFaitE. A.ФайтЕ. А.
Russian Federation

Junior Research Worker, Central Research Laboratory, Siberian State Medical University Address: 634050, Tomsk, Moscovsky Trakt, 2/18; tel.: (3822) 52-83-97

fa1len@mail.ruSiberian State Medical University, Tomsk, Russian FederationСибирский государственный медицинский университет, Томск, Российская Федерация14052013685Vestnik Rossiiskoi akademii medetsinskikh nauk / Annals of the Russian academy of medical sciencesВестник Российской академии медицинских наук667007082015Copyright ©; 1970, "Paediatrician" Publishers LLCCopyright ©; 1970, Издательство "Педиатръ"1970"Paediatrician" Publishers LLCИздательство "Педиатръ"https://vestnikramn.spr-journal.ru/jour/article/view/188

This work shows comparative characteristics of immunogenic properties of the extract of Opisthorchis felineus in different severity of asthma using antigen presenting dendritic cells in vitro. New data on the effect of Opisthorchis felineus on the expression of surface markers of dendritic cells (CD209, HLA-DR, CD83, CD86) were received. Pronounced expression of CD209, CD86 and HLA-DR on the surface of dendritic cells in mild and severe asthma compared with healthy individuals was shown. In the stimulation of dendritic cells with extract Opisthorchis felineus in vitro weakening of CD86 expression in mild and in severe asthma was found. CD86 molecule may be a regulatory factor in the co-stimulation of dendritic cells which allows us to consider it as a potential pharmacological target for the treatment of allergic diseases.

Дана сравнительная характеристика иммуногенных свойств экстракта Opisthorchis felineus при бронхиальной астме различной степени тяжести с использованием антигенпрезентирующих дендритных клеток in vitro. Получены новые данные о влиянии Opisthorchis felineus на экспрессию поверхностных маркеров дендритных клеток (CD209, HLA-DR, CD83, CD86). Выявлена более выраженная экспрессия молекул CD209, CD86 и HLA-DR на поверхности дендритных клеток при легкой и тяжелой астме по сравнению с показателями здоровых лиц. В условиях стимуляции дендритных клеток экстрактом Opisthorchis felineus in vitro установлено ослабление экспрессии CD86 как при легкой, так и при тяжелой астме. Молекула CD86 может быть регуляторным фактором в костимуляции дендритных клеток, что позволяет рассматривать ее в качестве возможной фармакологической мишени для терапии аллергических болезней.

dendritic cellsOpisthorchis felineusbronchial asthmaimmune responseдендритные клеткиOpisthorchis felineusбронхиальная астмаиммунный ответ1. Dudziak D., Kamphorst A. O., Heidkamp G. F., Buchholz V.R., Trumpfheller C., Yamazaki S., Cheong C., Liu K., Lee H.W., Park C.G., Steinman R.M., Nussenzweig M.C. Differential antigen processing by dendritic cell subsets in vivo. Science. 2007; 315: 107–111.2. Agrawal D.K., Shao Z. Pathogenesis of allergic airway inflammation. Curr. Allergy Asthma Rep. 2010; 10: 39–48.3. Mathur M., Herrmann K., Qin Y., Gulmen F., Li X., Krimins R., Weinstock J., Elliott D., Bluestone J.A., Padrid P. CD28 interactions with either CD80 or CD86 are sufficient to induce allergic airway inflammation in mice. Am. J. Resp. Cell. Mol. Biol. 1999; 21: 498–509.4. Jeong Y. I., Kim S. H., Ju J. W., Cho S.H., Lee W.J., Park J.W., Park Y.M., Lee S.E. Clonorchis sinensis-derived total protein attenuates airway inflammation in murine asthma model by inducing regulatory T cells and modulating dendritic cell functions. Biochem. Biophys. Res. Commun. 2011; 407: 793–800.5. Eliseeva O.V., Kremer E.E., Ogorodova L.M., Fedorova O.S., Deev I.A. Cellular immune response in children with asthma combined with chronic opisthorchosis. Vopr. diagnostiki v pediatrii = Diagnostics in pediatrics. 2011; 2: 15–19.6. Evdokimova, T.A., Ogorodova L.M. Vliyanie Effect of chronic opisthorchosis on the clinical course and immune response in atopic asthma in children. Pediatriya = Pediatrics. 2005; 6: 12–14.7. Global Initiative for Asthma (GINA): Global strategy for Asthma man-agement and prevention [Electronic resource]. Update 2009. Mode of access: http://www.ginasthma.com/Guidelineitem.asp ??l1=2&l2=1&intId=60.8. Ryzhov S.V., Yur'eva K.S., Goremykin K.V., Korotkaya E.V., Saltykova I.V., Yakovleva Yu.A., Kulikov E.S., Fedorova O.S., Kremer E.E., Fattakhov N.S., Sazonov A.E. Adenosine-dependent regulation of expression of paracrine factors in human venous blood monocytes. Byull. sibirskoi meditsiny = Bulletin of Siberian medicine. 2011; 3: 54–62.9. Relloso M., Puig-Kröger A., Pello O.M., Rodríguez-Fernández J.L., de la Rosa G., Longo N., Navarro J., Muñoz-Fernández M.A., Sánchez-Mateos P., Corbí A.L. DC-SIGN (CD209) expression is IL 4 dependent and is negatively regulated by IFN, TGF beta, and anti-inflammatory agents. J. Immunol. 2002; 168: 2634–2643.10. Chen X. Q., Yang J., Hu S.P., Nie H.X., Mao G.Y., Chen H.B. Increased expression of CD86 and reduced production of IL 12 and IL 10 by monocyte-derived dendritic cells from allergic asthmatics and their effects on Th1- and Th2-type cytokine balance. Respiration. 2006; 73: 34–40.11. Balanescu A., Radu E., Nat R., Regalia T., Bojinca V., Predescu V., Predeteanu D. Co-stimulatory and adhesion molecules of dendritic cells in rheumatoid arthritis. J. Cell. Mol. Med. 2002; 6: 415–425.12. Jacobs B., Wuttke M., Papewalis C. Dendritic cells subtypes and in vitro generation of dendritic cells. Horm. Metabol. Res. 2008; 40: 99–107.13. Oliveira R.R., Gollob K.J., Figueiredo J.P. Schistosoma mansoni infection alters co-stimulatory molecule expression and cell activation in asthma. Microbes Infect. 2009; 11: 223–229.